Article (Scientific journals)
Characterization of two monoclonal antibodies that recognize linker region and carboxyl terminal domain of coronavirus nucleocapsid protein
Zhang, X.; Zhao, X.; Dong, Hui et al.
2016In PLoS ONE, 11 (9)
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Keywords :
B lymphocyte antigen; Article; Transmissible gastroenteritis virus; Western blotting
Abstract :
[en] The transmissible gastroenteritis virus (TGEV) nucleocapsid (N) protein plays important roles in the replication and translation of viral RNA. The present study provides the first description of two monoclonal antibodies (mAbs) (5E8 and 3D7) directed against the TGEV N protein linker region (LKR) and carboxyl terminal domain (CTD). The mAbs 5E8 and 3D7 reacted with native N protein in western blotting and immunofluorescence assay (IFA). Two linear epitopes,189 SVEQAVLAALKKLG202 and246 VTRFYGARSSSA257 , located in the LKR and CTD of TGEV N protein, respectively, were identified after truncating the protein and applying a peptide scanning technique. Using mAb 5E8, we observed that the N protein was expressed in the cytoplasm during TGEV replication and that the protein could be immunoprecipitated from TGEV-infected PK-15 cells. The mAb 5E8 can be applied for different approaches to diagnosis of TGEV infection. In addition, the antibodies represent useful tools for investigating the antigenic properties of the N protein. © 2016 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Zhang, X.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Zhao, X.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Dong, Hui ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)
Zhu, Y.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Shi, H.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Chen, J.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Shi, D.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Feng, L.;  State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
Language :
English
Title :
Characterization of two monoclonal antibodies that recognize linker region and carboxyl terminal domain of coronavirus nucleocapsid protein
Publication date :
2016
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science
Volume :
11
Issue :
9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 December 2020

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