Effects of oral contraception with ethinylestradiol and drospirenone on oxidative stress in women 18-35 years old.

[en] Background Oral contraceptives (OCs) with estrogens and progestins may affect oxidative stress (OS) status. Study design A group of 32 women using oral contraceptives (OCU) containing 0.03 mg ethinylestradiol and 3 mg drospirenone have been compared to a matched control group of 30 noncontraception users (NCU). Blood levels of antioxidants, trace elements and markers of lipid peroxidation were assessed by biochemical methods. A microarray analysis of whole blood mRNA levels of 200 genes involved in OS-dependant pathway was also performed. Results Levels of zinc, vitamin E and antibodies to oxidized low-density lipoproteins (LDLs) were not significantly different between the two groups. On the other hand, significant increases in the mean levels of lipid peroxides (+176%, p<.001), oxidized LDLs (+145%, p<.002), copper (+103%, p<.001), Cu/Zn ratio (+100%, p<.001) and a significant decrease in the mean level of β-carotene (−41%, p<.01) were observed in the OCU compared to NCU. There was a highly significant positive correlation between the lipid peroxide levels and the copper-to-zinc ratio. From the 200 genes tested by microarray, one coding for HSP70 was significantly up-regulated (log2 fold change=+ 0.45, p<.02) and one coding for inducible nitric oxide synthase significantly down-regulated (log2 fold change=−0.24, p<.05) in the OCU compared to the NCU. Conclusions The recently introduced combination of ethinylestradiol and drospirenone induced the heightening of lipid peroxidation correlated with high levels of copper, a situation that could be associated with increased cardiovascular risk.

Disciplines :

Reproductive medicine (gynecology, andrology, obstetrics)

Author, co-author :

De Groote, D.

PERRIER d'HAUTERIVE, Sophie ; Université de Liège - ULiège > Gynécologie-Obstétrique CHR

Pintiaux, Axelle ; Université de Liège - ULiège > Gynécologie-Obstétrique CHR

Balteau, Bénédicte ; Université de Liège - ULiège > Gynécologie-Obstétrique CHR

Gerday, Colette ; Université de Liège - ULiège > Gynécologie-Obstétrique CHR

Claesen, J.

Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement

Language :

English

Title :

Effects of oral contraception with ethinylestradiol and drospirenone on oxidative stress in women 18-35 years old.

Publication date :

2009

Journal title :

Contraception

ISSN :

0010-7824

Publisher :

Elsevier, New York, United States - New York

Volume :

Issue :

Pages :

187-93

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 October 2009

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