Reference : Effects of oral contraception with ethinylestradiol and drospirenone on oxidative str...
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
Effects of oral contraception with ethinylestradiol and drospirenone on oxidative stress in women 18-35 years old.
De Groote, D. [ > > ]
PERRIER d'HAUTERIVE, Sophie [Université de Liège - ULiège > > Gynécologie-Obstétrique CHR >]
Pintiaux, Axelle [Université de Liège - ULiège > > Gynécologie-Obstétrique CHR >]
Balteau, Bénédicte [Université de Liège - ULiège > > Gynécologie-Obstétrique CHR >]
Gerday, Colette [Université de Liège - ULiège > > Gynécologie-Obstétrique CHR >]
Claesen, J. [ > > ]
Foidart, Jean-Michel mailto [Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Yes (verified by ORBi)
New York
[en] Contraceptive pill ; Estrogens ; Progestins ; Reactive oxygen species ; Antioxidants
[en] Background
Oral contraceptives (OCs) with estrogens and progestins may affect oxidative stress (OS) status.

Study design
A group of 32 women using oral contraceptives (OCU) containing 0.03 mg ethinylestradiol and 3 mg drospirenone have been compared to a matched control group of 30 noncontraception users (NCU). Blood levels of antioxidants, trace elements and markers of lipid peroxidation were assessed by biochemical methods. A microarray analysis of whole blood mRNA levels of 200 genes involved in OS-dependant pathway was also performed.

Levels of zinc, vitamin E and antibodies to oxidized low-density lipoproteins (LDLs) were not significantly different between the two groups. On the other hand, significant increases in the mean levels of lipid peroxides (+176%, p<.001), oxidized LDLs (+145%, p<.002), copper (+103%, p<.001), Cu/Zn ratio (+100%, p<.001) and a significant decrease in the mean level of β-carotene (−41%, p<.01) were observed in the OCU compared to NCU. There was a highly significant positive correlation between the lipid peroxide levels and the copper-to-zinc ratio. From the 200 genes tested by microarray, one coding for HSP70 was significantly up-regulated (log2 fold change=+ 0.45, p<.02) and one coding for inducible nitric oxide synthase significantly down-regulated (log2 fold change=−0.24, p<.05) in the OCU compared to the NCU.

The recently introduced combination of ethinylestradiol and drospirenone induced the heightening of lipid peroxidation correlated with high levels of copper, a situation that could be associated with increased cardiovascular risk.

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