Bronchial thermoplasty decreases airway remodelling by blocking epithelium-derived heat shock protein-60 secretion and protein arginine methyltransferase-1 in fibroblasts.
[en] Bronchial thermoplasty (BT) is to date the only therapy that provides a lasting reduction in airway wall remodelling. However, the mechanism of action of BT is not well understood. This study aimed to characterise the changes of remodelling regulating signalling pathways by BT in asthma.Bronchoalveolar lavage fluid (BALF) was obtained from eight patients with severe asthma before and after BT. Primary bronchial epithelial cells were isolated from 23 patients before (n=66) and after (n=62) BT. Epithelial cell culture supernatant (Epi.S) was collected and applied to primary fibroblasts.Epithelial cells obtained from asthma patients after BT proliferated significantly faster compared with epithelial cells obtained before BT. In airway fibroblasts, BALF or Epi.S obtained before BT increased CCAAT enhancer-binding protein-β (C/EBPβ) expression, thereby downregulating microRNA-19a. This upregulated extracellular signal-regulated kinase-1/2 (ERK1/2) expression, protein arginine methyltransferase-1 (PRMT1) expression, cell proliferation and mitochondrial mass. BALF or Epi.S obtained after BT reduced the expression of C/EBPβ, ERK1/2, peroxisome proliferator-activated receptor-γ coactivator-1α (PGC1α), PRMT1 and mitochondrial mass in airway fibroblasts. Proteome and transcriptome analyses indicated that epithelial cell-derived heat shock protein-60 (HSP60) is the main mediator of BT effects on fibroblasts. Further analysis suggested that HSP60 regulated PRMT1 expression, which was responsible for the increased mitochondrial mass and α-smooth muscle actin expression by asthmatic fibroblasts. These effects were ablated after BT. These results imply that BT reduces fibroblast remodelling through modifying the function of epithelial cells, especially by reducing HSP60 secretion and subsequent signalling pathways that regulate PRMT1 expression.We therefore hypothesise that BT decreases airway remodelling by blocking epithelium-derived HSP60 secretion and PRMT1 in fibroblasts.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Sun, Qingzhu
Fang, Lei
Roth, Michael
Tang, Xuemei
Papakonstantinou, Eleni
Zhai, Weiqi
Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Heinen, Vincent
Schleich, Florence ; Université de Liège - ULiège > Département des sciences de la motricité > Département des sciences de la motricité
Bronchial thermoplasty decreases airway remodelling by blocking epithelium-derived heat shock protein-60 secretion and protein arginine methyltransferase-1 in fibroblasts.
Pare PD, Roberts CR, Bai TR, et al. The functional consequences of airway remodelling in asthma. Monaldi Arch Chest Dis 1997; 52: 589-596.
Elias JA. Airway remodelling in asthma. Unanswered questions. Am J Respir Crit Care Med 2000; 161: S168-S171.
Berair R, Saunders R, Brightling CE. Origins of increased airway smooth muscle mass in asthma. BMC Med 2013; 11: 145.
Weitoft M, Andersson C, Andersson-Sjoland A, et al. Controlled and uncontrolled asthma display distinct alveolar tissue matrix compositions. Respir Res 2014; 15: 67.
Salem IH, Boulet LP, Biardel S, et al. Long-term effects of bronchial thermoplasty on airway smooth muscle and reticular basement membrane thickness in severe asthma. Ann Am Thorac Soc 2016; 13: 1426-1428.
Chakir J, Haj-Salem I, Gras D, et al. Effects of bronchial thermoplasty on airway smooth muscle and collagen deposition in asthma. Ann Am Thorac Soc 2015; 12: 1612-1618.
Janssen LJ. Airway smooth muscle as a target in asthma and the beneficial effects of bronchial thermoplasty. J Allergy 2012; 2012: 593784.
Loxham M, Davies DE, Blume C. Epithelial function and dysfunction in asthma. Clin Exp Allergy 2014; 44: 1299-1313.
Lambrecht BN, Hammad H. The airway epithelium in asthma. Nat Med 2012; 18: 684-692.
Prakash YS, Halayko AJ, Gosens R, et al. An Official American Thoracic Society Research Statement: Current Challenges Facing Research and Therapeutic Advances in Airway Remodelling. Am J Respir Crit Care Med 2017; 195: e4-e19.
Boulet LP. Airway remodelling in asthma: update on mechanisms and therapeutic approaches. Curr Opin Pulm Med 2018; 24: 56-62.
Pretolani M, Bergqvist A, Thabut G, et al. Effectiveness of bronchial thermoplasty in patients with severe refractory asthma: clinical and histopathologic correlations. J Allergy Clin Immunol 2017; 139: 1176-1185.
Denner DR, Doeing DC, Hogarth DK, et al. Airway inflammation after bronchial thermoplasty for severe asthma. Ann Am Thorac Soc 2015; 12: 1302-1309.
O'Reilly A, Browne I, Watchorn D, et al. The efficacy and safety of bronchial thermoplasty in severe persistent asthma on extended follow-up. QJM 2018; 111: 155-159.
DeVries A, Vercelli D. Epigenetic mechanisms in asthma. Ann Am Thorac Soc 2016; 13: Suppl. 1, S48-S50.
Kidd CD, Thompson PJ, Barrett L, et al. Histone modifications and asthma. The interface of the epigenetic and genetic landscapes. Am J Respir Cell Mol Biol 2016; 54: 3-12.
Sun Q, Liu L, Wang H, et al. Constitutive high expression of protein arginine methyltransferase 1 in asthmatic airway smooth muscle cells is caused by reduced microRNA-19a expression and leads to enhanced remodelling. J Allergy Clin Immunol 2017; 140: 510-524.
Wei H, Mundade R, Lange KC, et al. Protein arginine methylation of non-histone proteins and its role in diseases. Cell Cycle 2014; 13: 32-41.
Sun Q, Liu L, Roth M, et al. PRMT1 upregulated by epithelial proinflammatory cytokines participates in COX2 expression in fibroblasts and chronic antigen-induced pulmonary inflammation. J Immunol 2015; 195: 298-306.
Sun QZ, Yang XD, Zhong B, et al. Upregulated protein arginine methyltransferase 1 by IL-4 increases eotaxin-1 expression in airway epithelial cells and participates in antigen-induced pulmonary inflammation in rats. J Immunol 2012; 188: 3506-3512.
Sun Q, Liu L, Mandal J, et al. PDGF-BB induces PRMT1 expression through ERK1/2 dependent STAT1 activation and regulates remodelling in primary human lung fibroblasts. Cell Signal 2016; 28: 307-315.
Liu L, Sun Q, Bao R, et al. Specific regulation of PRMT1 expression by PIAS1 and RKIP in BEAS-2B epithelia cells and HFL-1 fibroblasts in lung inflammation. Sci Rep 2016; 6: 21810.
Sun Q, Fang L, Tang X, et al. TGF-β upregulated mitochondria mass through the SMAD2/3→C/EBPβ→PRMT1 signal pathway in primary human lung fibroblasts. J Immunol 2019; 202: 37-47.
Crosby LM, Waters CM. Epithelial repair mechanisms in the lung. Am J Physiol Lung Cell Mol Physiol 2010; 298: L715-L731.
Yan Y, Hanse EA, Stedman K, et al. Transcription factor C/EBP-beta induces tumor-suppressor phosphatase PHLPP2 through repression of the miR-17-92 cluster in differentiating AML cells. Cell Death Differ 2016; 23: 1232-1242.
Girodet PO, Allard B, Thumerel M, et al. Bronchial smooth muscle remodelling in nonsevere asthma. Am J Respir Crit Care Med 2016; 193: 627-633.
Girodet PO, Ozier A, Bara I, et al. Airway remodelling in asthma: new mechanisms and potential for pharmacological intervention. Pharmacol Ther 2011; 130: 325-337.
Aravamudan B, Kiel A, Freeman M, et al. Cigarette smoke-induced mitochondrial fragmentation and dysfunction in human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 2014; 306: L840-L854.
Kol A, Lichtman AH, Finberg RW, et al. Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells. J Immunol 2000; 164: 13-17.
Sell H, Poitou C, Habich C, et al. Heat shock protein 60 in obesity: effect of bariatric surgery and its relation to inflammation and cardiovascular risk. Obesity 2017; 25: 2108-2114.
Marker T, Sell H, Zillessen P, et al. Heat shock protein 60 as a mediator of adipose tissue inflammation and insulin resistance. Diabetes 2012; 61: 615-625.
Park MJ, Kim DI, Lim SK, et al. Thioredoxin-interacting protein mediates hepatic lipogenesis and inflammation via PRMT1 and PGC-1alpha regulation in vitro and in vivo. J Hepatol 2014; 61: 1151-1157.
Shen NY, Ng SY, Toepp SL, et al. Protein arginine methyltransferase expression and activity during myogenesis. Biosci Rep 2018; 38: BSR20171533.