Fatty Acid Profiles, Antioxidant and Phenolic Contents of Oils Extracted from Acacia polycantha and Azadirachta indica (Neem) Seeds using Green Solvents
Saha Tchinda, Jean-Bosco; Fetngna Tchebe, Tatiana Mbitnkeu; Abdou, Tchoukouaet al.
2021 • In Journal of Food Processing and Preservation, 45 (2), p. 15115
[en] The purpose of the present study was to evaluate the composition of fatty acids, antioxidant and phenolic compounds in Acacia polycantha and Azadirachta indica seed extracts for their potential uses in nutrition. Extractions of oil using several techniques (Sohxlet, ultrasound and microwave) in several solvents were carried out and the oils were characterised. Total phenolic content ranged between 4.22 and 31.48 mg GAE/g. Antioxidant activity (CE50) ranged between 1.95 and 22.91 mg/mL. Oleic and linoleic acids were the major unsaturated fatty acids while palmitic and stearic acids were the major saturated fatty acids in both oils. 3‐hydrobenzoic acid, resveratrol rutin, and flavan were identified by HPLC‐DAD at high contents while chlorogenic acid, vanillic acid, syringic acid, epicatechin, p‐coumaric acid, transferulic acid, ellagic acid, rutin, cinnamic acid, chlorogenic acid, caffeic acid were lower in Neem oil. In case of A. polycantha oil, the percentages of ellagic acid and rutin were high.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Saha Tchinda, Jean-Bosco
Fetngna Tchebe, Tatiana Mbitnkeu
Abdou, Tchoukoua
Cheumani Yona, Arnaud Maxime
Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Ndikontar, Maurice Kor
Richel, Aurore ; Université de Liège - ULiège > Département GxABT > SMARTECH
Language :
English
Title :
Fatty Acid Profiles, Antioxidant and Phenolic Contents of Oils Extracted from Acacia polycantha and Azadirachta indica (Neem) Seeds using Green Solvents
Publication date :
2021
Journal title :
Journal of Food Processing and Preservation
ISSN :
0145-8892
eISSN :
1745-4549
Publisher :
Wiley-Blackwell, Hoboken, United States - New Jersey
Acharya, P., Ahmad, M. S., & Nayak, B. (2017). Competence of biopesticide and neem in agriculture. International Journal of Environment, Agriculture and Biotechnology, 2(6), 2958–2964. https://doi.org/10.22161/ijeab/2.6.23
Adewoye, T. L., & Ogunleye, O. O. (2012). Optimization of neem seed oil extraction process using response surface methodology. Journal of Natural Sciences Research, 2(6), 66–75.
Akhila, A., & Rani, K. (1999). Chemistry of the neem tree (Azadirachta indica A. Juss.). In W. Herz, H. Falk, G. W. Kirby, R. E. Moore, & C. Tamm (Eds.), Fortschritte der Chemie Organischer Naturstoffe/Progress in the chemistry of organic natural products (Vol. 78). Springer.
Alara, O. R., Abdurahman, N. H., & Olalere, O. A. (2020). Ethanolic extraction of flavonoids, phenolics and antioxidants from Vernonia amygdalina leaf using two-level factorial design. Journal of King Saud University – Science. https://doi.org/10.1016/j.jksus.2017.08.00132(1), 7–16.
Ambrosino, P., Fresa, R., Fogliano, V., Monti, M. S., & Ritieni, A. (1999). Extraction of azadirachtin A from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS. Journal of Agricultural and Food Chemistry, 47, 5252–5256. https://doi.org/10.1021/jf9905368
Banerji, R., Chowdhury, A. R., Misra, G., & Nigam, S. K. (1988). Chemical composition of Acacia seeds. Journal of the American Oil Chemists’ Society, 65, 1959–1960.
Bettaieb, R. I., Aidi, W. W., Ben, K. S., Bourgou, S., Saidani, T. M., Ksouri, R., & Fauconnier, M. L. (2019). Bioactive compounds and antioxidant activity of Pimpinellaanisum L. accessions at different ripening stages. Scientia Horticulturae, 246, 453–461. https://doi.org/10.1016/j.scienta.2018.11.016
Biswas, K., Chattopadhyay, I., Banerjee, K. R., & Bandyopadhyay, U. (2002). Biological activities and medicinal properties of Neem (Azadirachta indica). Current Science, 82(11), 1336–1345.
Boskou, D., Tsimidou, M., & Blekas, G. (2006). Part 2: Chemical properties, healt effects. 5: Polar Phenolic compounds. In D. Boskou (Ed.), Olive oil chemistry and technology (2nd ed.). AOCS.
Burkill, H. M. (1985). The useful plants of West Tropical Africa, Vol. 3. https://plants.jstor.org/stable/10.5555/al.ap.upwta.3_282
Castejón, N., Luna, P., & Señoráns, J. F. (2018). Alternative oil extraction methods from Echiumplanta gineum L. seeds using advanced techniques and green solvents. Food Chemistry, 244, 75–82.
Diedhiou, D. (2017). Fractionnement analytique de la graine de Neem (Azadirachta indica A. Juss.) et de la graine de dattier du désert (Balanites aegyptiaca L.) - Valorisation des constituants de la graine de Neem par bioraffinage (p. 291). Thèse de Doctorat, INP.
Djenontin, T. S., Wotto, V. D., Avlessi, F., Lozano, P., Sohounhloué, D. K. C., & Pioch, D. (2012). Composition of Azadirachta indica and Carapa procera (Meliaceae) seed oils and cakes obtained after oil extraction. Industrial Crops and Products, 38, 39–45. https://doi.org/10.1016/j.indcrop.2012.01.005
Fanali, C., Della Posta, S., Vilmercati, A., Dugo, L., Russo, M., Petitti, T., Mondello, L., & de Gara, L. (2018). Extraction, analysis, and antioxidant activity evaluation of phenolic compounds in different Italian extra-virgin olive oils. Molecules, 23, 3249. https://doi.org/10.3390/molecules23123249
Fathi-Achachlouei, B., Azadmard-Damirchi, S., Zahedi, Y., & Shaddel, R. (2019). Microwave pretreatment as a promising strategy for increment of nutraceutical content and extraction yield of oil from milk thistle seed. Industrial Crops & Products, 128, 527–533. https://doi.org/10.1016/j.indcrop.2018.11.034
Faye, M. (2010). Nouveau procédé de fractionnement de la graine de Neem (Azadirachta indica A. Juss) sénégalais: Production d’un biopesticide d’huile et de tourteau (p. 266). Thèse de Doctorat, INP.
Fernandes, R. S., Barreiros, L., Oliveira, F. R., Cruz, A., Prudêncio, C., Oliveira, I. A., Pinho, C., Santos, N., & Morgado, J. (2019). Chemistry, bioactivities, extraction and analysis of azadirachtin: State-of-the-art. Fitoterapia, 134, 141–150. https://doi.org/10.1016/j.fitote.2019.02.006
Fotso, W. G., Na-Iya, J., Mbaveng, T. A., Ango, Y. P., Demirtas, I., Kuete, V., Yeboah, S., Ngameni, B., Efferth, T., & Ngadjui, T. B. (2018). Polyacanthoside A, a new oleanane-type triterpenoidsaponin with cytotoxic effects from the leaves of Acacia polyacantha (Fabaceae). Natural Product Research (Formerly Natural Product Letters), 33(24), 3521–3526.
Goldsmith, D. C., Vuong, V. Q., Stathopoulos, E. C., Roach, D. P., & Scarlett, J. C. (2018). Ultrasound increases the aqueous extraction of phenolic compounds with high antioxidant activity from olive pomace. LWT - Food Science and Technology, 89, 284–290. https://doi.org/10.1016/j.lwt.2017.10.065
Gossé, B., Amissa, A. A., Adjé, A. F., Niamké, B. F., Ollivier, D., & Ito, Y. (2005). Analysis of components of Neem (Azadirachta indica) oil by diverse chromatographic techniques. Journal of Liquid Chromatography and Related Technologies, 28, 2225–2233.
Isman, B. M., Koul, O., Luczynshi, A., & Kaminski, J. (1990). Insecticidal and antifeedant bioactivities of neem oils and their relationship to azadirachtin content. Journal of Agricultural and Food Chemistry, 38(6), 1406–1411. https://doi.org/10.1021/jf00096a024
Isopencu, G., Stroescu, M., Brosteanu, A., Chira, N., Pârvulescu, C. O., Busuioc, C., & Stoica-Guzun, A. (2019). Optimization of ultrasound and microwave assisted oil extraction from sea buckthorn seeds by response surface methodology. Journal of Food Process Engineering, 42, e12947. https://doi.org/10.1111/jfpe.12947
Kalogeropoulos, N., & Tsimidou, Z. M. (2014). Antioxidants in Greek Virgin Olive Oils, Review. Antioxidants, 3, 387–413. https://doi.org/10.3390/antiox3020387
Kaushik, N. (2002). Determination of azadirachtin and fatty acid methyl esters of Azadirachta indica seeds by HPLC and GLC. Analytical and Bioanalytical Chemistry, 374, 1199–1204. https://doi.org/10.1007/s00216-002-1638-7
Kaushik, N., & Vir, S. (2000). Variations in fatty acid composition of Neem seeds collected from the Rajasthan state of India. Biochemical Society Transactions, 28, 880–882. https://doi.org/10.1042/bst0280880
Khan, R., Srivastava, R., Khan, A. M., Alam, P., & Abdin, M. Z. (2012). Variation in oil content and fatty acid composition of the seed oil of Acacia species collected from the northwest zone of India. Journal of the Science of Food and Agriculture, 92, 2310. https://doi.org/10.1002/jsfa.5627
Koudoro, Y. A., Agbangnan, D. C. P., Yèhouénou, B., Tchobo, F. P., Alitonou, G. A., Avlessi, F., Akoègninou, A., & Sohounhloué, K. C. D. (2015). Chemical characterization and biological activities of extracts from two plants (Cissus quadrangularis and Acacia polyacantha) used in veterinary medicine in Benin. Journal of Pharmacognosy and Phytochemistry, 3(6), 91–96.
Kozłowska, M., & Gruczyńska, E. (2018). Comparison of the oxidative stability of soybean and sunflower oils enriched with herbal plant extracts. Chemical Papers, 72, 2607–2615. https://doi.org/10.1007/s11696-018-0516-5
Lanza, B., & Ninfali, P. (2020). Antioxidants in extra virgin olive oil and table olives: Connections between agriculture and processing for health choices. Antioxidants, 9, 41. https://doi.org/10.3390/antiox9010041
Lee, S. M., Klocke, A. J., Barnby, A. M., Yamasaki, R. B., & Baladrin, F. M. (1991). Insecticidal Constituents of Azadirachta indica and Melia azadirach (Meliaceae). In P. A. Hedin (Ed.), Naturally occurring pest bioregulators. ACS Symposium Series No. 449 (p. 293). American Chemical Society.
Liauw, M. Y., Natan, F. A., Widiyanti, P., Ikasari, D., Indraswati, N., & Soetaredjo, F. E. (2008). Extraction of Neem oil (Azadirachta indica A. Juss) using n-hexane and ethanol: Studies of oil quality, kinetic and thermodynamic. ARPN Journal of Engineering and Applied Sciences, 3(3), 49–54.
Liu, Y., Zhang, H., & Wei, S. (2015). Ultrasonic-assisted extraction of pigments from Hylocereus undatus flowers: Optimization, antioxidant activity, and HPLC analysis. RSC Advances, 5, 46598–46607. https://doi.org/10.1039/C5RA04089B
Llompart, M., Garcia-Jares, C., & Celeiro, M. (2019). Extraction/microwave-assisted extraction. Worsfold Paul Poole Colin Townshend Alan & Miró Manuel In Encyclopedia of analytical science (3rd ed., pp. 67–77). https://doi.org/10.1016/B0-12-369397-7/00693-2Academic Press.
Mambe, T. F., Na-Iya, J., Fotso, W. G., Ashu, F., Ngameni, B., Ngadjui, T. B., Beng, P. V., & Kuete, V. (2019). Antibacterial and antibiotic modifying potential of crude extracts, fractions, and compounds from Acacia polyacantha Willd against MDR gram-negative bacteria. Evidence-Based Complementary and Alternative Medicine, 1–13. https://doi.org/10.1155/2019/7507549
Momchilova, S., Antonova, D., Marekov, I., Kuleva, L., & Nikolova-Damyanova, B. (2007). Fatty Acids, triacylglycerols, and sterols in neem oil (Azadirachta indica A. Juss) as determined by a combination of chromatographic and spectral techniques. Journal of Liquid Chromatography & Related Technologies, 30, 11–25.
Muñoz-Valenzuela, S., Ibarra-López, A. A., Rubio-Silva, M. L., Valdez-Dávila, H., & Borboa-Flores, J. (2007). Neem tree morphology and oil content. In J. Janick, & A. Whipkey (Eds.), New crops and New uses (pp. 126–128). ASHS Press.
Nde, B. D., Boldor, D., & Astete, C. (2015). Optimization of microwave-assisted extraction parameters of Neem (Azadirachta indica A. Juss) oil using the Doehlert’s experimental design. Industrial Crops and Products, 65, 233–240. https://doi.org/10.1016/j.indcrop.2014.12.015
Iwu, M.M., Obidoa, O., & Anazodo, M. (1986). Biochemical mechanism of the antimalarial activity of Azadirachta indica leaf extract. Pharmacological Research Communications, 18(1), 81–91.
Orhevba, B. A., Chukwu, O., Osunde, Z. D., & Ogwuagwu, V. (2013). Influence of moisture content on the yield of mechanically expressed Neem seed kernel oil. Academic Research International, 4(5), 252–257.
Perrier, A., Delsart, C., Boussetta, N., Grimi, N., Citeau, M., & Vorobiev, E. (2017). Effect of ultrasound and green solvents addition on the oil extraction efficiency from rapeseed flakes. Ultrasonics-Sonochemistry, 39, 58–65. https://doi.org/10.1016/j.ultsonch.2017.04.003
Prat, D., Wells, A., Hayler, J., Sneddon, H., McElroy, C. R., Abou-Shehada, S., & Dunn, J. P. (2016). CHEM21 selection guide of classical and less classical solvents. Green Chemistry, 18(1), 288–296. https://doi.org/10.1039/C5GC01008J
Rahmani, H. A., Almatroudi, A., Alrumaihi, F., & Khan, A. A. (2018). Pharmacological and therapeutic potential of neem (Azadirachta indica). Pharmacognosy Reviews, 12(24), 250–255. https://doi.org/10.4103/phrev.phrev_8_18
Rao, P. S., Subramanayam, G., & Sridhar, P. R. (2019). Quercetin 3-galactoside from Azadirachta indica. Journal of Advances in Molecular Biology, 3(1), 1–8. https://doi.org/10.22606/jamb.2019.31001
Riar, S. S., Devakumar, C., Ilavazhagan, G., Bardhan, J., Kain, A. K., Thomas, P., Singh, R., & Singh, B. (1990). Volatile fraction of neem oil as a spermicide. Contraception, 42(4), 479–487. https://doi.org/10.1016/0010-7824(90)90054-Y
Saha Tchinda, J.-B., Abia, D., Dumarçay, S., Ndikontar Kor, M., Gérardin, P., Noah Ngamveng, J., & Perrin, D. (2013). Antioxidant activities, total phenolic contents and chemical compositions of extracts from four Cameroonian woods: padouk (Pterocarpus soyauxii Taubb), tali (Erythrophleum suaveolens), moabi (Baillonella toxisperma), and movingui (Distemonanthus benthamianus). Industrial Crops and Products, 41, 71–77.
Saha Tchinda, J.-B., Ndikontar, K. M., Fouda Belinga, A. D., Mounguengui, S., Njankouo, J. M., Durmaçay, S., & Gerardin, P. (2018). Inhibition of fungi with wood extractives and natural durability of five Cameroonian wood species. Industrial Crops & Products, 123, 183–191. https://doi.org/10.1016/j.indcrop.2018.06.078
Shewale, S., & Rathod, K. V. (2018). Extraction of total phenolic content from Azadirachta indica or (Neem) leaves: Kinetics study. Preparative Biochemistry and Biotechnology, 48(4), 312–320. https://doi.org/10.1080/10826068.2018.1431784
Szterk, A., Roszko, M., Sosińska, D., Derewiaka, D., & Lewicki, P. P. (2010). Chemical composition and oxidative stability of selected plant oils. Journal of the American Oil Chemists’ Society, 87, 637–645. https://doi.org/10.1007/s11746-009-1539-4
Tsimidou, M., Blekas, G., & Boskou, D. (2003). Olive oil. In B. Caballero, L. Trugo, & P. Finglas (Eds.), Encyclopedia of food science, food technology and nutrition (pp. 4252–4260). Academic Press.
Ulbricht, T. L. K., & Southgate, D. A. T. (1991). Coronary heart disease: Seven dietary factors. The Lancet, 38, 985–992. https://doi.org/10.1016/0140-6736(91)91846-M
Waterman, C., Smith, A. R., Pontiggia, L., & Der Marderosian, A. (2010). Anthelmintic screening of Sub-Saharan African plants used in traditional medicine. Journal of Ethnopharmacology, 127, 755–759. https://doi.org/10.1016/j.jep.2009.11.025
Yan, J., Shen, Y., Wang, Y., Luan, X., Guo, M., & Li, C. (2016). The effects of different extraction methods on the physicochemical properties and antioxidant activity of Amygdalus pedunculatus seed oil. Journal of Applied Botany and Food Quality, 89, 135–141. https://doi.org/10.5073/JABFQ.2016.089.016
Youzbachi, N., Elfalleh, W., Tlili, N., Gregoire, S., Berdeaux, O., Salles, C., Triki, S., Mohamed-Larbi, K., Abdelhamid, K., & Nasri, N. (2012). Unexploited Acacia cyanophylla seeds: Potential food sources of ω6 fatty acids and antioxidants? Journal of the Science of Food and Agriculture, 92, 1526. https://doi.org/10.1002/jsfa.4737
Youzbachi, N., Trabelsi, H., Elfalleh, W., Khaldi, A., Nasri, N., & Tlili, N. (2019). Fatty acids and triacylglycerols composition from Tunisian Acacia species seed oil. Arabian Journal of Chemistry. https://doi.org/10.1016/j.arabjc.2015.08.02012(8), 33202–3308.
Zhan-Jun, L., Feng-jian, Y., Lei, Y., & Yuan-Gang, Z. (2016). Ultrasonic extraction of oil from Caesalpinia spinosa (Tara) seeds. Journal of Chemistry, 2016, 6. Article ID 1794123. https://doi.org/10.1155/2016/1794123
Zhong, J., Wang, Y., Yang, R., Liu, X., Yang, Q., & Qin, X. (2018). The application of ultrasound and microwave to increase oil extraction from Moringa oleifera seeds. Industrial Crops & Products, 120, 1–10. https://doi.org/10.1016/j.indcrop.2018.04.028
