Unpublished conference/Abstract (Scientific congresses and symposiums)
Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows affected by Parietal Fibrinous Peritonitis after Caesarean Section
Djebala, Salem ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Médecine interne des ruminants et des porcs
Evrard, Julien; Regional Association of Health and Animal Identification > Gestion et Prévention de Santé > Gestion et Prévention de Santé > DMV
Gregoire, Fabien; Regional Association of Health and Animal Identification > Gestion et Prévention de Santé > Gestion et Prévention de Santé > DMV
Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Bayrou, Calixte ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP)
Moula, Nassim ; Université de Liège - ULiège > Dpt. de gestion vétérinaire des Ressources Animales (DRA) > GIGA Research (AFT)
Sartelet, Arnaud ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP)
Bossaert, Philippe ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Thériogenologie des animaux de production
Language :
English
Title :
Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows affected by Parietal Fibrinous Peritonitis after Caesarean Section
Publication date :
20 November 2020
Event name :
7the FARAH-Day
Event organizer :
ULiège - Université de Liège
Event place :
Liege, Belgium
Event date :
november 27th 2020
Commentary :
The aim of the current study was to identify the pathogens that are potentially involved in parietal fibrinous peritonitis (PFP). PFP is a relatively common complication of cattle celiotomy, characterized by an accumulation of exudate in a fibrinous capsule within the abdominal or pelvic cavity; its aetiology is poorly understood.
We have studied 72 cases of PFP, confirmed by a standard diagnostic protocol. Blood were collected to evaluate the presence of antibodies for Mycoplasma bovis (M. bovis), Coxiella burnetii (C. burnetii) and Bovine Herpesvirus 4 (BoHV4) by enzyme-linked immunosorbent assays. Peritoneal exudate samples were obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of M. bovis, C. burnetii and BoHV4 using real time polymerase chain reaction (qPCR).
Bacteriological culture was positive in most of peritoneal samples (59/72); Trueperella pyogenes (T. pyogenes) (51/72) and Escherichia coli (E. coli) (20/72) were the most frequently identified. For BoHV4, the majority of cows showed a positive serology and qPCR result (56/72 and 49/72, respectively), in contrast to M. bovis (17/72 and 6/72, respectively) and C. burnetii (15/72 and 6/72, respectively), who were less frequently detected (p<0.0001).
Our study proves that PFP can no longer be qualified as a sterile inflammation, since most PFP samples yielded a positive bacteriology and qPCR. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows affected by PFP. The exact role of these germs in the pathogenesis of PFP is not yet elucidated and requires further studies
