Screening for Viral Pathogens in African Simian Bushmeat Seized at A French Airport

Africa; France; Haplorhini; Airports; Animals; Bacteriophages; Commerce; DNA, Viral; Food Microbiology; Genome, Viral; Mass Screening; Meat; Metagenome; Microscopy, Fluorescence; RNA, Viral

Abstract :

[en] Illegal bushmeat traffic is an important threat to biodiversity conservation of several endangered species and may contribute to the emergence and spread of infectious diseases in humans. The hunting, manipulation and consumption of wildlife-based products, especially those of primate origin, may be a threat to human health; however, few studies have investigated the role of bushmeat trade and consumption as a potential source of human infections to date. In this study, we report the screening of viral pathogens in African simian game seized by French customs at Toulouse Blagnac Airport. Epifluorescence microscopy revealed the presence of virus-like particles in the samples, and further metagenomic sequencing of the DNA and RNA viromes confirmed the presence of sequences related to the Siphoviridae, Myoviridae and Podoviridae bacteriophage families; some of them infecting bacterial hosts that could be potentially pathogenic for humans. To increase the sensitivity of detection, twelve pan-generic PCRs targeting several viral zoonoses were performed, but no positive signal was detected. A large-scale inventory of bacteria, viruses and parasites is urgently needed to globally assess the risk for human health of the trade, manipulation and consumption of wildlife-related bushmeat. © 2016 Blackwell Verlag GmbH

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Temmam, S.; URMITE UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine de la Timone, Aix-Marseille Université, Marseille, France

Davoust, B.; URMITE UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine de la Timone, Aix-Marseille Université, Marseille, France

Chaber, Anne-Lise ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.

Lignereux, Y.; National Veterinary School, INP, Toulouse Cedex 03, France, Natural History Museum, Toulouse, France

Michelle, C.; URMITE UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine de la Timone, Aix-Marseille Université, Marseille, France

Monteil-Bouchard, S.; URMITE UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine de la Timone, Aix-Marseille Université, Marseille, France

Raoult, D.; URMITE UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine de la Timone, Aix-Marseille Université, Marseille, France

Desnues, C.; URMITE UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine de la Timone, Aix-Marseille Université, Marseille, France

Language :

English

Title :

Screening for Viral Pathogens in African Simian Bushmeat Seized at A French Airport

Publication date :

2017

Journal title :

Transboundary and Emerging Diseases

ISSN :

1865-1674

eISSN :

1865-1682

Publisher :

Blackwell Publishing Ltd

Volume :

Issue :

Pages :

1159-1167

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 November 2020

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