A comparative study on the leishmanicidal activity of the L-amino acid oxidases BjussuLAAO-II and BmooLAAO-II isolated from Brazilian Bothrops snake venoms
[en] This study aims to examine whether two L-amino acid oxidases isolated from Bothrops snake venom (SV-LAAOs) were cytotoxic to Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis, two causative agents of leishmaniasis, which is an endemic disease in tropical and subtropical countries. The SV-LAAOs BjussuLAAO-II and BmooLAAO-II were isolated from Bothrops jararacussu and Bothrops moojeni venom, respectively, through a three-step chromatography process that used molecular exclusion, hydrophobic interaction, and affinity columns. BmooLAAO-II is a new SV-LAAO isoform that we isolated in this study. The purified BjussuLAAO-II and BmooLAAO-II had high L-amino acid oxidase-specific activity: 3481.17 and 4924.77 U/mg/min, respectively. Both SV-LAAOs were strongly cytotoxic to the two Leishmania species, even at low concentrations. At the same concentration, BjussuLAAO-II and BmooLAAO-II exerted different cytotoxic effects on the parasites. We reported for the first time that the SV-LAAOs suppressed cell proliferation and altered the mitochondrial membrane potential of the two Leishmania species. Surprisingly, BjussuLAAO-II increased the intracellular reactive oxygen species production only in L. (L.) amazonensis, while BmooLAAO-II increased the intracellular reactive oxygen species production only in L. (V.) braziliensis, indicating that these SV-LAAOs had a certain specificity of action.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Barbosa, Luana Gonçalves; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Costa, Tassia Rafaela; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Borges, Isabela Pacheco; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Costa, Mônica Soares; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Carneiro, Anna Cecília; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Borges, Bruna Cristina; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Osteoimmunology and Tumor Immunology, Institute of Biomedical Sciences
Silva, Marcelo José Barbosa; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Osteoimmunology and Tumor Immunology, Institute of Biomedical Sciences
Gobbi Amorim, Fernanda ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique
Yoneyama, Kelly Aparecida Geraldo; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Rodrigues, Veridiana de Melo; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
Sampaio, Suely Vilela; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo – USP, Ribeirão Preto, SP, Brazil. > Department of Clinical Analyses, Toxicology and Food Sciences
Rodrigues, Renata Santos; Federal University of Uberlândia – UFU, Uberlândia, MG, Brazil > Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology
A comparative study on the leishmanicidal activity of the L-amino acid oxidases BjussuLAAO-II and BmooLAAO-II isolated from Brazilian Bothrops snake venoms
Publication date :
2021
Journal title :
International Journal of Biological Macromolecules
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