Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.

Bricmont, Noémie; Benchimol, Lionel; POIRRIER, Anne-Lise; Grignet, Christine; Seaton, Claire; Chilvers, Mark A.; Seghaye, Marie-Christine; Louis, Renaud; Lefèbvre, Philippe; KEMPENEERS, Céline

doi:10.3791/61949

Article (Scientific journals)

Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.

Bricmont, Noémie; Benchimol, Lionel; POIRRIER, Anne-Lise et al.

2020 • In Journal of Visualized Experiments, (165)

Peer reviewed

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https://hdl.handle.net/2268/253092

DOI
10.3791/61949

PubMed
33226018

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Keywords :

Primary Ciliary Dyskinesia; Coronavirus; Videomicroscopy; Sample processing; Ciliopathy; Diagnosis

Abstract :

[en] Primary Ciliary Dyskinesia (PCD) is a genetic motile ciliopathy, leading to significant otosinopulmonary disease. PCD diagnosis is often missed or delayed due to challenges with different diagnostic modalities. Ciliary videomicroscopy, using Digital High-Speed Videomicroscopy (DHSV), one of the diagnostic tools for PCD, is considered the optimal method to perform ciliary functional analysis (CFA), comprising of ciliary beat frequency (CBF) and beat pattern (CBP) analysis. However, DHSV lacks standardized, published operating procedure for processing and analyzing samples. It also uses living respiratory epithelium, a significant infection control issue during the COVID-19 pandemic. To continue providing a diagnostic service during this health crisis, the ciliary videomicroscopy protocol has been adapted to include adequate infection control measures. Here, we describe a revised protocol for sampling and laboratory processing of ciliated respiratory samples, highlighting adaptations made to comply with COVID-19 infection control measures. Representative results of CFA from nasal brushing samples obtained from 16 healthy subjects, processed and analyzed according to this protocol, are described. We also illustrate the importance of obtaining and processing optimal quality epithelial ciliated strips, as samples not meeting quality selection criteria do now allow for CFA, potentially decreasing the diagnostic reliability and the efficiency of this technique.

Disciplines :

Pediatrics

Author, co-author :

Bricmont, Noémie ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie cardiologique et pneumologique

Benchimol, Lionel ; Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale

POIRRIER, Anne-Lise ; Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale

Grignet, Christine ; Université de Liège - ULiège > Protection et hygiène du travail (SUPHT)

Seaton, Claire

Chilvers, Mark A.

Seghaye, Marie-Christine ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie cardiologique et pneumologique

Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie

Lefèbvre, Philippe ; Université de Liège - ULiège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie

KEMPENEERS, Céline ; Centre Hospitalier Universitaire de Liège - CHU > Département de Pédiatrie > Service de pédiatrie

Language :

English

Title :

Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.

Publication date :

2020

Journal title :

Journal of Visualized Experiments

eISSN :

1940-087X

Issue :

165

Peer reviewed :

Peer reviewed

Available on ORBi :

since 24 November 2020

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