Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.

Bricmont, Noémie; Benchimol, Lionel; POIRRIER, Anne-Lise; Grignet, Christine; Seaton, Claire; Chilvers, Mark A.; Seghaye, Marie-Christine; Louis, Renaud; Lefèbvre, Philippe; KEMPENEERS, Céline

doi:10.3791/61949

Article (Scientific journals)

Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.

Bricmont, Noémie; Benchimol, Lionel; POIRRIER, Anne-Lise et al.

2020 • In Journal of Visualized Experiments, (165)

Peer reviewed

Permalink
https://hdl.handle.net/2268/253092

DOI
10.3791/61949

PubMed
33226018

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

Bricmont JOVE (2020).pdf

Publisher postprint (3.42 MB)

Request a copy

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Primary Ciliary Dyskinesia; Coronavirus; Videomicroscopy; Sample processing; Ciliopathy; Diagnosis

Abstract :

[en] Primary Ciliary Dyskinesia (PCD) is a genetic motile ciliopathy, leading to significant otosinopulmonary disease. PCD diagnosis is often missed or delayed due to challenges with different diagnostic modalities. Ciliary videomicroscopy, using Digital High-Speed Videomicroscopy (DHSV), one of the diagnostic tools for PCD, is considered the optimal method to perform ciliary functional analysis (CFA), comprising of ciliary beat frequency (CBF) and beat pattern (CBP) analysis. However, DHSV lacks standardized, published operating procedure for processing and analyzing samples. It also uses living respiratory epithelium, a significant infection control issue during the COVID-19 pandemic. To continue providing a diagnostic service during this health crisis, the ciliary videomicroscopy protocol has been adapted to include adequate infection control measures. Here, we describe a revised protocol for sampling and laboratory processing of ciliated respiratory samples, highlighting adaptations made to comply with COVID-19 infection control measures. Representative results of CFA from nasal brushing samples obtained from 16 healthy subjects, processed and analyzed according to this protocol, are described. We also illustrate the importance of obtaining and processing optimal quality epithelial ciliated strips, as samples not meeting quality selection criteria do now allow for CFA, potentially decreasing the diagnostic reliability and the efficiency of this technique.

Disciplines :

Pediatrics

Author, co-author :

Bricmont, Noémie ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie cardiologique et pneumologique

Benchimol, Lionel ; Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale

POIRRIER, Anne-Lise ; Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale

Grignet, Christine ; Université de Liège - ULiège > Protection et hygiène du travail (SUPHT)

Seaton, Claire

Chilvers, Mark A.

Seghaye, Marie-Christine ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie cardiologique et pneumologique

Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie

Lefèbvre, Philippe ; Université de Liège - ULiège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie

KEMPENEERS, Céline ; Centre Hospitalier Universitaire de Liège - CHU > Département de Pédiatrie > Service de pédiatrie

Language :

English

Title :

Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.

Publication date :

2020

Journal title :

Journal of Visualized Experiments

eISSN :

1940-087X

Issue :

165

Peer reviewed :

Peer reviewed

Available on ORBi :

since 24 November 2020

Statistics

Number of views

103 (23 by ULiège)

Number of downloads

9 (9 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Chilvers, M.A., Rutman, A., O’Callaghan, C. Ciliary beat pattern is associated with specific ultrastructural defects in primary ciliary dyskinesia. Journal of Allergy Clinical Immunology. 112 (3), 518-524 (2003).
Werner, C., Onnebrink, J.G., Omran, H. Diagnosis and management of primary ciliary dyskinesia. Cilia. (SUPPLEMENT 1), 1-9 (2015).
Kempeneers, C., Chilvers, M.A. To beat, or not to beat, that is question! The spectrum of ciliopathies. Pediatric Pulmonology. 53 (8), 1122-112 (2018).
Lucas, J.S. et al. European Respiratory Society guidelines for the diagnosis of primary ciliary dyskinesia. The European Respiratory Journal. 49 (1), ERJ-01090-2016 (2017).
Knowles, M.R., Zariwala, M., Leigh, M. Primary Ciliary Dyskinesia. Clinics in chest medicine. 37 (3), 449-461 (2016).
Shapiro, A.J. et al. Diagnosis, monitoring, and treatment of primary ciliary dyskinesia: PCD foundation consensus recommendations based on state of the art review. Pediatric Pulmonology. (2016).
Fitzgerald, D.A., Shapiro, A.J. When to suspect primary ciliary dyskinesia in children. Paediatric Respiratory Reviews. (2016).
Shoemark, A., Dell, S., Shapiro, A., Lucas, J.S. ERS and ATS diagnostic guidelines for primary ciliary dyskinesia: similarities and differences in approach to diagnosis. European Respiratory Journal. 54 (3) (2019).
Mirra, V., Werner, C., Santamaria, F. Primary ciliary dyskinesia: An update on clinical aspects, genetics, diagnosis, and future treatment strategies. Frontiers in Pediatrics. 5 (June), 1-13 (2017).
Ardura-Garcia, C. et al. Registries and collaborative studies for primary ciliary dyskinesia in Europe. European Respiratory Journal Open Research. 6 (2) (2020).
Leigh, M.W. et al. Clinical features and associated likelihood of primary ciliary dyskinesia in children and adolescents. Annals of the American Thoracic Society. (2016).
Chilvers, M.A., O’Callaghan, C. Analysis of ciliary beat pattern and beat frequency using digital high speed imaging: comparison with the photomultiplier and photodiode methods. Thorax. 55 (4), 314-317 (2000).
Kempeneers, C., Seaton, C., Garcia Espinosa, B., Chilvers, M.A. Ciliary functional analysis: Beating a path towards standardization. Pediatric Pulmonology. 54 (10), 1627-1638 (2019).
Barbato, A. et al. Primary ciliary dyskinesia: a consensus statement on diagnostic and treatment approaches in children. The European respiratory journal. 34 (6), 1264-1276 (2009).
Raidt, J. et al. Ciliary beat pattern and frequency in genetic variants of primary ciliary dyskinesia. European Respiratory Journal. 44 (6), 1579-1588 (2014).
Kempeneers, C., Seaton, C., Chilvers, M.A. Variation of Ciliary Beat Pattern in Three Different Beating Planes in Healthy Subjects. Chest. 151 (5), 993-1001 (2017).
Götzinger, F. et al. COVID-19 in children and adolescents in Europe: a multinational, multicentre cohort study. The Lancet Child & Adolescent Health. (2020).
Yang, J. et al. Prevalence of comorbidities and its effects in coronavirus disease 2019 patients: A systematic review and meta-analysis. International Journal of Infectious Diseases. 94, 91-95 (2020).
Brough, H.A. et al. Managing childhood allergies and immunodeficiencies during respiratory virus epidemics – The 2020 COVID-19 pandemic: A statement from the EAACI-section on pediatrics. Pediatric Allergy and Immunology. 31 (5), 442-448 (2020).
Zou, L. et al. SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients. The New England journal of medicine. 382 (12), 1177-1179 (2020).
van Doremalen, N. et al. Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1. The New England journal of medicine. 382 (16), 1564-1567 (2020).
Tran, K., Cimon, K., Severn, M., Pessoa-Silva, C.L., Conly, J. Aerosol generating procedures and risk of transmission of acute respiratory infections to healthcare workers: a systematic review. PloS one. 7 (4), e35797 (2012).
Van Gerven, L. et al. Personal protection and delivery of rhinologic and endoscopic skull base procedures during the COVID-19 outbreak. Rhinology. 58 (3), 289-294 (2020).
Marty, F.M., Chen, K., Verrill, K.A. How to Obtain a Nasopharyngeal Swab Specimen. New England Journal of Medicine. 382 (22), e76 (2020).
Petruzzi, G. et al. COVID-19: Nasal and oropharyngeal swab. Head & Neck. 42 (2020).
George, A., Prince, M., Coulson, C. Safe nasendoscopy assisted procedure in the post-COVID-19 pandemic era. Clinical Otolaryngology. (2020).
Hirst, R.A. et al. Culture of primary ciliary dyskinesia epithelial cells at air-liquid interface can alter ciliary phenotype but remains a robust and informative diagnostic aid. PLoS ONE. 9 (2) (2014).
Jorissen, M., Willems, T., Van der Schueren, B. Ciliary function analysis for the diagnosis of primary ciliary dyskinesia: advantages of ciliogenesis in culture. Acta oto-laryngologica. 120 (2), 291-295 (2000).
Thomas, B., Rutman, A., O’Callaghan, C. Disrupted ciliated epithelium shows slower ciliary beat frequency and increased dyskinesia. European Respiratory Journal. 34 (2), 401-404 (2009).
Chilvers, M.A., Rutman, A., O’Callaghan, C. Functional analysis of cilia and ciliated epithelial ultrastructure in healthy children and young adults. Thorax. 58 (4), 333-338 (2003).
Stannard, W.A., Chilvers, M.A., Rutman, A.R., Williams, C.D., O’Callaghan, C. Diagnostic testing of patients suspected of primary ciliary dyskinesia. American Journal of Respiratory and Critical Care Medicine. 181 (4), 307-314 (2010).
Boon, M. et al. Primary ciliary dyskinesia: critical evaluation of clinical symptoms and diagnosis in patients with normal and abnormal ultrastructure. Orphanet Journal of Rare Diseases. 9 (1), 11 (2014).
Armengot, M., Milara, J., Mata, M., Carda, C., Cortijo, J. Cilia motility and structure in primary and secondary ciliary dyskinesia. American Journal of Rhinology & Allergy. 24 (3), 175-180 (2010).
Papon, J.F. et al. Quantitative analysis of ciliary beating in primary ciliary dyskinesia: a pilot study. Orphanet Journal of Rare Diseases. 7 (1), 78 (2012).
Wallmeier, J. et al. Mutations in CCNO and MCIDAS lead to a mucociliary clearance disorder due to reduced generation of multiple motile cilia. Molecular and Cellular Pediatrics. 2 (Suppl 1), A15 (2015).
Boon, M. et al. MCIDAS mutations result in a mucociliary clearance disorder with reduced generation of multiple motile cilia. Nature Communications. 5 (6), 4418 (2014).
Shapiro, A.J. et al. Diagnosis of Primary Ciliary Dyskinesia. An Official American Thoracic Society Clinical Practice Guideline. American Journal of Respiratory and Critical Care Medicine. 197 (12), e24-e39 (2018).
Rubbo, B. et al. Accuracy of high-speed video analysis to diagnose primary ciliary dyskinesia. Chest. Feb 28 (Epub ahead of print), S0012-3692(19)30205-3 (2019).
Horani, A., Ferkol, T.W. Advances in the Genetics of Primary Ciliary Dyskinesia. Chest. 154 (3), 645-652 (2018).
MacCormick, J., Robb, I., Kovesi, T., Carpenter, B. Optimal biopsy techniques in the diagnosis of primary ciliary dyskinesia. The Journal of Otolaryngology. 31 (1), 13-17 (2002).
Jackson, C.L. et al. Accuracy of diagnostic testing in primary ciliary dyskinesia. European Respiratory Journal. 47 (3), 837-848 (2016).
Jackson, C.L., Goggin, P.M., Lucas, J.S. Ciliary Beat Pattern Analysis Below 37°C May Increase Risk of Primary Ciliary Dyskinesia Misdiagnosis. Chest. 142 (2), 543-544 (2012).
Green, A., Smallman, L.A., Logan, A.C., Drake-Lee, A.B. The effect of temperature on nasal ciliary beat frequency. Clinical otolaryngology and allied sciences. 20 (2), 178-180 (1995).
Clary-Meinesz, C.F., Cosson, J., Huitorel, P., Blaive, B. Temperature effect on the ciliary beat frequency of human nasal and tracheal ciliated cells. Biology of the Cell. 76 (3), 335-338 (1992).
Smith, C.M. et al. ciliaFA: a research tool for automated, high-throughput measurement of ciliary beat frequency using freely available software. Cilia. 1 (1), 14 (2012).
Sisson, J.H., Stoner, J. a., Ammons, B. a., Wyatt, T. a. All-digital image capture and whole-field analysis of ciliary beat frequency. Journal of Microscopy. 211 (Pt 2), 103-111 (2003).
Blanchon, S. et al. Deep phenotyping, including quantitative ciliary beating parameters, and extensive genotyping in primary ciliary dyskinesia. Journal of Medical Genetics. jmedgenet-2019-106424 (2019).
Feriani, L. et al. Assessing the Collective Dynamics of Motile Cilia in Cultures of Human Airway Cells by Multiscale DDM. Biophysical Journal. 113 (1), 109-119 (2017).
Sears, P.R., Thompson, K., Knowles, M.R., Davis, C.W. Human airway ciliary dynamics. American Journal of Physiology-Lung Cellular and Molecular Physiology. 304 (3), 170-183 (2013).
Quinn, S.P. et al. Automated identification of abnormal respiratory ciliary motion in nasal biopsies. Science translational medicine. 7 (299), 299ra124 (2015).