Article (Scientific journals)
Effects of Naturally Occurring Mutations in Bovine Leukemia Virus 50 -LTR and Tax Gene on Viral Transcriptional Activity
Pluta, Aneta; Willems, Luc; Douville, Renée N. et al.
2020In Pathogens, 9 (10), p. 836
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Keywords :
retrovirus; bovine leukemia virus (BLV); long terminal repeat (LTR); Tax protein; transactivation; transcription factor; proviral load; sequence variants; primary isolates
Abstract :
[en] Bovine leukemia virus (BLV) is a deltaretrovirus infecting bovine B cells and causing enzootic bovine leucosis (EBL). The long terminal repeat (LTR) plays an indispensable role in viral gene expression. The BLV Tax protein acts as the main transactivator of LTR-driven transcription of BLV viral genes. The aim of this study was to analyze mutations in the BLV LTR region and tax gene to determine their association with transcriptional activity. LTRs were obtained from one hundred and six BLV isolates and analyzed for their genetic variability. Fifteen variants were selected and characterized based on mutations in LTR regulatory elements, and further used for in vitro transcription assays. Reporter vectors containing the luciferase gene under the control of each variant BLV promoter sequence, in addition to variant Tax expression vectors, were constructed. Both types of plasmids were used for cotransfection of HeLa cells and the level of luciferase activity was measured as a proxy of transcriptional activity. Marked differences in LTR promoter activity and Tax transactivation activity were observed amongst BLV variants. These results demonstrate that mutations in both the BLV LTR and tax gene can affect the promoter activity, which may have important consequences on proviral load, viral fitness, and transmissibility in BLV-infected cattle.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Pluta, Aneta;  National Veterinary Research Institute, Puławy
Willems, Luc  ;  Université de Liège - ULiège > Cancer-Cellular and Molecular Epigenetics
Douville, Renée N.
Kuzmak, Jacek;  National Veterinary Research Institute, Puławy
Language :
English
Title :
Effects of Naturally Occurring Mutations in Bovine Leukemia Virus 50 -LTR and Tax Gene on Viral Transcriptional Activity
Publication date :
13 October 2020
Journal title :
Pathogens
eISSN :
2076-0817
Publisher :
Molecular Diversity Preservation International (MDPI), Basel, Switzerland
Volume :
9
Issue :
10
Pages :
836
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NCN - Narodowe Centrum Nauki [PL]
Fund for Scientific Research at the National Veterinary Research Institute, Puławy, Poland
Available on ORBi :
since 23 November 2020

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