[en] Optimal activation of T lymphocytes depends on TCR interaction with peptide/MHC complexes in conjunction with costimulatory signals, which are delivered by specialized cells called antigen-presenting-cells (APC). The population of APC is heterogeneous and includes dendritic cells, B cells and macrophages. The family of dendritic cells (DC) is widely distributed in tissues and plays a major role in the induction of primary T-dependent immune responses. The aim of this paper was to isolate and characterize dendritic cells from cattle. Two methods are described that have been used to isolate dendritic cells from bovine peripheral blood. One method involves sequential depletion of other cells, adherence and isolation of low buoyant density cells on Metrizamide column. The second involves enrichment of cells displaying receptors for plasma fibronectin, followed by adherence and separation on Metrizamide. Both preparations were characterized morphologically by flow cytometry and functionally. Both procedures produced enriched populations that did not express molecules typical of T cells (CD3, CD4, CD8, WC1), B cells (sIg, CD21) and monocytes (CD14, Fc gamma 2R). Procedure 2 yielded cells with a typical veiled DC morphology that were highly effective at stimulating allogeneic T cells. Procedure 1 yielded cells that did not have the veiled morphology and were less effective in the MLR which may represent a more immature stage.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Renjifo, X.; Université Libre de Bruxelles - ULB > Laboratoire de Physiologie Animale
Howard, C.; Compton Laboratory > Institute for Animal Health
Kerkhofs, P.; National Veterinary Research Institute
Denis, M.; SmithKline Beecham Biologicals
Urbain, J.; Université Libre de Bruxelles - ULB > Laboratoire de Physiologie Animale
Moser, M.; Université Libre de Bruxelles - ULB > Laboratoire de Physiologie Animale
Pastoret, Paul-Pierre ; Université de Liège - ULiège > Médecine vétérinaire et santé animale > Virology-Immunology
Language :
English
Title :
Purification and characterization of bovine dendritic cells from peripheral blood
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Ardavin, C., Wu, L., Li, C.L., Shortman, K., 1993. Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population. Nature 362, 761-763.
Austyn, A.M., Hankins, D.F., Larsen, C.P., Morris, P.J., Roake, J.A., 1994. Isolation and characterization of dendritic cells from mouse heart and kidney. J. Immunol. 152, 2401-2410.
Bender, A., Sapp., M., Schuler, G., Steinman, R.M., Bhardwaj, N., 1996. Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Meth. 196, 121-135.
Bevilacqua, M.P., Amrani, O., Mosesson, M.W., Bianco, C., 1981. Receptors for cold-insoluble globulin (plasma fibronectin) on human monocytes. J. Exp. Med. 153, 42-60.
Bujdoso, R.J., Hopkins, B.M., Dutia, P., Young, P., McConnell, I., 1989. Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage. J. Exp. Med. 170, 1285-1301.
Cumberbatch, M., Kimber, I., 1995. Tumor necrosis factor-α is required for accumulation of dendritic cells in draining lymph nodes and for optimal contact sensitization. Immunology 84, 31-35.
De Smedt, T., Pajak, B., Muraille, E., Lespagnard, L., Heinen, E., De Baetselier, P., Urbain, J., Leo, O., Moser, M., 1996. Regulation of dendritic cells numbers and maturation by lipolysaccharide in vivo. J. Exp. Med. 184, 1413-1424.
Freudenthal, P.S., Steinman, R.M., 1990. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc. Natl. Acad. Sci. USA. 87, 7698-7702.
Heufler, C., Koch, F., Schuler, G., 1988. Granulocyte/macrophage colony-stimulating factor and interleukin I mediate the maturation of epidermal Langerhans into potent immunostimulatory dendritic cells. J. Exp. Med. 167, 700-705.
Holt, P.G., Oliver, J., Bilyk, N., McMenamin, C., Kraal, G., Thepen, T., 1993. Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages. J. Exp. Med. 177, 397-407.
Howard, C., Naessens, J., 1993. General summary of workshop findings for cattle: Section 4. Vet. Immunol. Immunopathol. 39, 25-48.
Inaba, K., Witmer-Pack, M., Inaba, M., Hathcock, K.S., Sukuta, H., Azuma, M., Yagita, H., Okumura, K., Linsley, P.S., Ikehara, S., Muramatsu, S., Hodes, R.J., Steinman, R.M., 1994. The tissue distribution of the B7-2 costimulator in mice: Abundant expression on dendritic cells in situ and during maturation in vitro. J. Exp. Med. 180, 1849-1860.
Koch, F., Heufler, C., Kampgen, E., Schneeweiss, D., Bock, G., Schuler, G., 1990. Tumor necrosis factor α maintains the viability of murine epidermal Langerhans cells in culture, but in contrast to granulocyte/macrophage colony-stimulating factor, without inducing their functional maturation. J. Exp. Med. 171, 159-171.
Koch, F., Trockenbacher, B., Kämpgen, E., Grauer, O., Stössel, H., Livingstone, A.M., Schuler, G., Romani, N., 1995. Antigen processing in populations of mature murine dendritic cells is caused by subsets of incompletely matured cells. J. Immunol. 155, 93-100.
Kudo, S., Matsuno, K., Ezaki, T., Ogawa, M., 1997. A novel migration pathway for rat dendritic cells from the blood: Hepatic sinusoids-lymph translocation. J. Exp. Med. 185, 777-784.
Larsen, C.P., Steinman, R.M., Witmer-Pack, M., Hankins, D.F., Morris, P.J., Austyn, J.M., 1990. Migration and maturation of Langerhans cells in skin transplants and explants. J. Exp. Med. 172, 1483-1493.
Larsen, C.P., Ritchie, S.C., Pearson, T.C., Linsley, P.S., Lowry, R.P., 1992. Functional expression of the costimulatory molecule, B7/BB1, on murine dendritic cell population. J. Exp. Med. 176, 1215-1220.
Larsen, C.P., Ritchie, S.C., Hendrix, R., Linsley, P.S., Hathcock, K.S., Hodes, R.J., Lowry, R.P., Pearson, T.C., 1994. Regulation of immunostimulatory function and costimulatory molecule (B7-1 and B7-2) expression on murine dendritic cells. J. Immunol. 152, 5208-5219.
Liu, L.M., MacPherson, G.G., 1993. Antigen acquisition by dendritic cells: Intestinal dendritic cells acquire antigen administered orally and can prime naïve T cells in vivo. J. Exp. Med. 177, 1299-1307.
McKeever, D.J., MacHugh, N.D., Goddeeris, B.M., Awino, E., Morrison, I.W., 1991. Bovine afferent lymph veiled cells differ from blood monocytes in phenotype and accessory function. J. Immunol. 147, 3703-3709.
McKeever, D.J., Morrison, W.I., 1992. Afferent lymph veiled cells prime CD4+ T cell responses in vivo. Eur. J. Immunol. 22, 3057-3061.
O'Doherty, U., Steinman, R.M., Peng, M., Cameron, P.U., Gezelter, S., Kopeloff, I., Swiggard, W.J., Pope, M., Bhardwaj, N., 1993. Dendritic cells freshly isolated from peripheral blood express CD4 and mature into typical immunostimulatory dendritic cells after culture in monocyte-conditioned medium. J. Exp. Med. 178, 1067-1078.
O'Doherty, U., Peng, M., Gezelter, W., Swiggard, M., Betjes, N., Bhardwaj, N., Steinman, R.M., 1994. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature. Immunology 82, 487-493.
Romani, N., Lenz, A., Glassel, H., Stossel, H., Stanzl, U., Majoic, O., Fritsch, P., Schuler, G., 1989. Cultured human Langerhans cells resemble lymphoid dendritic cells in phenotype and function. J. Invest. Dermatol. 93, 600-609.
Romani, N., Gruner, S., Brang, D., Kämpgen, E., Lenz, A., Trockenbacher, B., Konwalinka, G., Steinman, R.M., Schluer, G., 1994. Proliferation dendritic cell progenitors in human blood. J. Exp. Med. 180, 83-93.
Schuler, G., Steinman, R.M., 1985. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161, 526-546.
Steinman, R.M., Cohn, Z.A., 1973. Identification of a novel cell type in peripheral lymphoid organs of mice: I. Morphology, quantification, tissue distribution. J. Exp. Med. 137, 1142.
Steinman, R.M., Gutchinov, B., Witmer, M.D., Nussenzweig, M.C., 1983. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157, 613-627.
Steinman, R.M., 1991. The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol. 9, 271-296.
Streilen, J.W., Grammer, S.F., 1989. In vitro evidence that Langerhans cells can adopt two functionally distinct forms capable of antigen presentation to T lymphocytes. J. Immunol. 143, 3925-3933.
Strober, W., Kelsall, B.L., 1996. Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's Patch. J. Exp. Med. 183, 237-247.
Symington, F., Brady, W., Linsley, P.S., 1993. Expression and function of B7 on human epidermal Langerhans cells. J. Immunol. 150, 1286-1295.
Thomas, R., Davis, L.S., Lipsky, P.E., 1993. Human peripheral blood dendritic cell subsets. Isolation and characterization of human peripheral blood dendritic cells. J. Immunol. 150, 821-834.
Van-Voorhis, W.C., Hair, L.S., Steinman, R.M., Kaplan, G., 1982. Human dendritic cells: enrichment and characterization from peripheral blood. J. Exp. Med. 155, 1172-1187.
Weissman, D., Li, Y., Ananworanich, J., Zhou, L., Adelsberger, J., Tedder, T.F., Baseler, M., Fauci, A., 1995. Three populations of cells with dendritic cells morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. 92, 826-830.
Winzler, C., Rovere, P., Rescigno, M., Granulocci, F., Penna, G., Adorini, L., Zimmermann, V.S., Davoust, J., Ricciardi-Castagnoli, P., 1997. Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures. J. Exp. Med. 185, 317-328.
Young, J.W., Koulova, L., Soergel, S.A., Clark, E.A., Steinman, R.M., 1992. The B7/BB1 antigen provides none of several costimulatory signals for the activation of CD4+ T lymphocytes by human blood dendritic cells in vitro. J. Clin. Invest. 90, 229-237.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.