Article (Scientific journals)
Nitrosomonas europaea-like bacteria detected as the dominant beta-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils
Carnol, Monique; Kowalchuk, G. A.; De Boer, W.
2002In Soil Biology and Biochemistry, 34, p. 1047-1050
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Keywords :
16S rDNA; DGGE; Picea abies; Quercus petraea; nitrification; dolomite lime
Abstract :
[en] Net nitrification in intact soil cores and the community of ammonia-oxidising bacteria were studied in acid Norway spruce (Picea abies (L.) Karst) and sessile oak (Quercus petraea (Matt. Lieb.)) soils (Haute Ardenne, east Belgium) 18 months after treatment with 5 t ha(-1) dolomite lime. Liming caused a significant increase in nitrification in the upper soil layers (0.15 m) of both stands. DGGE (denaturing gradient gel electrophoresis) profiling after P-subclass ammonia oxidiser-specific polymerase chain reaction (PCR), combined with hybridisation and sequencing of excised DGGE bands revealed a dominance of Nitrosomonas europaea-like sequences, independent of soil horizon, tree species and lime treatment. A minority Nitrosospira-like population was detected, which showed affinity to nitrosospiras previously detected in acid soil. These results contrast with several reports suggesting a dominance of Nitrosospira-like organisms among ammonia oxidiser communities in acid soils. (C) 2002 Elsevier Science Ltd. All rights reserved.
Disciplines :
Environmental sciences & ecology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Carnol, Monique  ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Ecologie végétale et microbienne
Kowalchuk, G. A.;  Netherlands Institute of Ecology - Centre for Terrestrial Ecology > Department of Plant-Microorganism Interactions
De Boer, W.;  Netherlands Institute of Ecology - Centre for Terrestrial Ecology > Department of Plant-Microorganism Interactions
Language :
English
Title :
Nitrosomonas europaea-like bacteria detected as the dominant beta-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils
Publication date :
2002
Journal title :
Soil Biology and Biochemistry
ISSN :
0038-0717
eISSN :
1879-3428
Publisher :
Pergamon-Elsevier Science Ltd, Oxford, United Kingdom
Volume :
34
Pages :
1047-1050
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
SSTC - Services Fédéraux des Affaires Scientifiques, Techniques et Culturelles [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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