Nitrosomonas europaea-like bacteria detected as the dominant beta-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils

Carnol, Monique; Kowalchuk, G. A.; De Boer, W.

doi:10.1016/S0038-0717(02)00039-1

Article (Scientific journals)

Nitrosomonas europaea-like bacteria detected as the dominant beta-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils

Carnol, Monique; Kowalchuk, G. A.; De Boer, W.

2002 • In Soil Biology and Biochemistry, 34, p. 1047-1050

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/25123

DOI
10.1016/S0038-0717(02)00039-1

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Keywords :

16S rDNA; DGGE; Picea abies; Quercus petraea; nitrification; dolomite lime

Abstract :

[en] Net nitrification in intact soil cores and the community of ammonia-oxidising bacteria were studied in acid Norway spruce (Picea abies (L.) Karst) and sessile oak (Quercus petraea (Matt. Lieb.)) soils (Haute Ardenne, east Belgium) 18 months after treatment with 5 t ha(-1) dolomite lime. Liming caused a significant increase in nitrification in the upper soil layers (0.15 m) of both stands. DGGE (denaturing gradient gel electrophoresis) profiling after P-subclass ammonia oxidiser-specific polymerase chain reaction (PCR), combined with hybridisation and sequencing of excised DGGE bands revealed a dominance of Nitrosomonas europaea-like sequences, independent of soil horizon, tree species and lime treatment. A minority Nitrosospira-like population was detected, which showed affinity to nitrosospiras previously detected in acid soil. These results contrast with several reports suggesting a dominance of Nitrosospira-like organisms among ammonia oxidiser communities in acid soils. (C) 2002 Elsevier Science Ltd. All rights reserved.

Disciplines :

Environmental sciences & ecology
Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Carnol, Monique ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Ecologie végétale et microbienne

Kowalchuk, G. A.; Netherlands Institute of Ecology - Centre for Terrestrial Ecology > Department of Plant-Microorganism Interactions

De Boer, W.; Netherlands Institute of Ecology - Centre for Terrestrial Ecology > Department of Plant-Microorganism Interactions

Language :

English

Title :

Nitrosomonas europaea-like bacteria detected as the dominant beta-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils

Publication date :

2002

Journal title :

Soil Biology and Biochemistry

ISSN :

0038-0717

eISSN :

1879-3428

Publisher :

Pergamon-Elsevier Science Ltd, Oxford, United Kingdom

Volume :

Pages :

1047-1050

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

SSTC - Services Fédéraux des Affaires Scientifiques, Techniques et Culturelles
F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 06 October 2009

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