[en] A significant decrease in adherence rates of Mycoplasma bovis to bovine bronchial epithelial (BBE) cells has been observed after passage of the organism in artificial medium. Analysis of the proteins expressed by M. bovis isolate 2610 by two-dimensional (2-D) electrophoresis demonstrated differences between the cells harvested after the 7th and 116th passage. Three silver-stained prominent spots observed in 2-D electrophoretic separation of protein extracts of the lower-passaged cells were considerably less strongly expressed in the sample from higher-passaged cells. These spots had a molecular mass of approximately 24 kDa and an isoelectric point of about 5. The mass spectrometry analysis of these trypsin-sensitive proteins led to their identification as a unique new member of the Vsps family of membrane-associated proteins. Serum from a mouse immunized with these proteins significantly reduced adherence of M. bovis to BBE cells. This result underlines the function of this new Vsp in adherence of M. bovis to host cells. (c) 2005 Elsevier SAS. All rights reserved.
Disciplines :
Microbiology Veterinary medicine & animal health
Author, co-author :
Thomas, Anne; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Dizier, Isabelle ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Gevaert, Kris; Flanders Interuniversity Institute of Biotechnology > Department of Medical Protein Research
Van Damme, Jozef; Flanders Interuniversity Institute of Biotechnology > Department of Medical Protein Research
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Linden, Annick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage
Language :
English
Title :
Identification by two-dimensional electrophoresis of a new adhesin expressed by a low-passaged strain of Mycoplasma bovis
Publication date :
2005
Journal title :
Research in Microbiology
ISSN :
0923-2508
eISSN :
1769-7123
Publisher :
Elsevier, Paris, France
Volume :
156
Issue :
5-6,
Pages :
713-718
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
COST - European Cooperation in Science and Technology
Funders :
Ministère Fédéral de l'Agriculture F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE] DG RDT - Commission Européenne. Direction Générale de la Recherche et de l'Innovation [BE]
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