Article (Scientific journals)
Anti-Influenza A Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species
Dam Van Phai; Desmecht, Daniel; Garigliany, Mutien-Marie et al.
2019In Journal of Interferon and Cytokine Research, 39 (5), p. 274-282
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Keywords :
Mx1; buffalo; type I/III interferons
Abstract :
[en] Type I/III interferons provide powerful and universal innate intracellular defense mechanisms against viruses. Among the antiviral effectors induced, Mx proteins of some species appear as key components of defense against influenza A viruses. It is expected that such an antiviral protein must display a platform dedicated to the recognition of said viruses. In an attempt to identify such platform in human MxA, an evolution-guided approach capitalizing on the antagonistic arms race between MxA and its viral targets and the genomic signature it left on primate genomes revealed that the surface-exposed so-called "loop L4", which protrudes from the compact structure of the MxA stalk, is a hotspot of recurrent positive selection. Since MxA is archetypic of Mx1 proteins in general, we reasoned that the L4 loop also functions as a recognition platform for influenza viruses in the Mx1 proteins of other species that had been exposed to the virus for ever. In this study, the anti-influenza activity of 5 distinct mammalian Mx1 proteins was measured by comparing the number of viral nucleoprotein-positive cells 7 h after infection in a sample of 100,000 cells expected to contain both Mx1-positive and Mx1-negative cell subpopulations. The systematic depletion (P < 0.001) of virus nucleoprotein-positive cells among equine, bubaline, porcine, and bovine Mx1-expressing cell populations compared with Mx-negative cells suggests a strong anti-influenza A activity. Looking for common anti-influenza signature elements in the sequence of these Mx proteins, we found that an aromatic residue at positions 561 or 562 in the L4 loop seems critical for the anti-influenza function and/or specificity of mammalian Mx1.
Precision for document type :
Critical notes/Edition
Disciplines :
Biotechnology
Author, co-author :
Dam Van Phai ;  Université de Liège - ULiège > Doct. sc. vété. (paysage)
Desmecht, Daniel ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Garigliany, Mutien-Marie  ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie générale et autopsies
BUI TRAN ANH, Dao;  Vietnam National University of Agriculture > Veterinary Pathology > Pathology
Van Laere, Anne-Sophie  ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Language :
English
Title :
Anti-Influenza A Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species
Alternative titles :
[en] Belgium
Publication date :
13 May 2019
Journal title :
Journal of Interferon and Cytokine Research
ISSN :
1079-9907
eISSN :
1557-7465
Publisher :
Mary Ann Liebert, Liège, United States - New York
Volume :
39
Issue :
5
Pages :
274-282
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Académie de Recherche et d'Enseignement Supérieur (Belgique). Coopération au Développement - ARES. CCD
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