Impact of the adsorbent material on volatile metabolites during in vitro and in vivo bio-sampling

[en] The increased attraction of biological volatile compounds has opened the route to a wide variety of sampling techniques, amongst which trap tubes packed with adsorbent materials are commonly used. Many types of adsorbent materials are available and the choice of the adsorbent can impact the obtained results in untargeted analysis. Therefore, a proper combination of the adsorbent material and the sample is necessary to increase the robustness and reproducibility of biological studies. In this study, the sampling performance of thermal desorption tubes with six common adsorbent material combinations, i.e., Tenax® TA, Tenax® TA/Carbopack™ B, Tenax® TA/Sulficarb, Tenax® TA/Carbograph™ 5TD, Tenax® TA/Carbograph™ 1TD/Carboxen® 1003, and Carboxen® 1013/Carbograph™ 5TD, was evaluated in two different setups: in vitro and in vivo sampling. The in vitro setup consisted of the headspace dynamic extraction of spiked serum, and a mixture of 19 standards was evaluated in terms of response and reproducibility. The in vivo setup consisted into two parts: the first one was based the evaluation of the standard mixture, which was flash-vaporised into Tedlar® bags containing exhaled breath; the second part was based on the longitudinal monitoring of breath metabolites originating from a beverage intake (i.e., brewed coffee), over a 90 min time period. The tubes were all desorbed and analysed in a comprehensive two-dimensional gas chromatography system coupled to a high-resolution time-of-flight mass spectrometer (GC×GC-HR ToF MS). In both sampling setups, the widest analytes coverage and the overall best extraction yield on the selected compounds were obtained using Tenax® TA, followed by Tenax® TA/Carbopack™ B. Tenax® TA provided the highest sampling reproducibility with 12 %RSD, 10 %RSD and < 5 %RSD of the response during the experiments using the in vitro setup, the in vivo setup, and during the longitudinal tracking, respectively.

Disciplines :

Chemistry

Author, co-author :

Franchina, Flavio ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique

Zanella, Delphine ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique

Dejong, Thibaut ; Université de Liège - ULiège > Form. doct. sc. (chimie - paysage)

Focant, Jean-François ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique

Language :

English

Title :

Impact of the adsorbent material on volatile metabolites during in vitro and in vivo bio-sampling

Publication date :

January 2021

Journal title :

Talanta

ISSN :

0039-9140

eISSN :

1873-3573

Publisher :

Elsevier, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 14 September 2020

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Bibliography

Zhang, Z., Ma, Y., Li, G., Progress on the analytical methodology for biological volatile organic compounds. Anal. Methods. 5 (2013), 20–29, 10.1039/c2ay26082d.
Mochalski, P., King, J., Klieber, M., Unterkofler, K., Hinterhuber, H., Baumann, M., Amann, A., Blood and breath levels of selected volatile organic compounds in healthy volunteers. Analyst 138 (2013), 2134–2145, 10.1039/c3an36756h.
Lawal, O., Ahmed, W.M., Nijsen, T.M.E., Goodacre, R., Fowler, S.J., Exhaled breath analysis: a review of ‘breath-taking’ methods for off-line analysis. Metabolomics 13 (2017), 1–16, 10.1007/s11306-017-1241-8.
Buszewski, B., Kesy, M., Ligor, T., Amann, A., Human exhaled air analytics: biomarkers of diseases. Biomed. Chromatogr. 21 (2007), 553–566, 10.1002/bmc.835.
Schuhmacher, R., Krska, R., Weckwerth, W., Goodacre, R., Metabolomics and metabolite profiling. Anal. Bioanal. Chem. 405 (2013), 5003–5004, 10.1007/s00216-013-6939-5.
Bojko, B., Reyes-Garcés, N., Bessonneau, V., Goryński, K., Mousavi, F., Souza Silva, E.A., Pawliszyn, J., Solid-phase microextraction in metabolomics. TrAC Trends Anal. Chem. (Reference Ed.) 61 (2014), 168–180, 10.1016/j.trac.2014.07.005.
Zhang, Q.H., Di Zhou, L., Chen, H., Wang, C.Z., Xia, Z.N., Yuan, C.S., Solid-phase microextraction technology for in vitro and in vivo metabolite analysis. TrAC Trends Anal. Chem. (Reference Ed.) 80 (2016), 57–65, 10.1016/j.trac.2016.02.017.
Drabińska, N., Azeem, H.A., Krupa-Kozak, U., A targeted metabolomic protocol for quantitative analysis of volatile organic compounds in urine of children with celiac disease. RSC Adv. 8 (2018), 36534–36541, 10.1039/c8ra07342b.
Silva, C., Perestrelo, R., Silva, P., Capelinha, F., Tomás, H., Câmara, J.S., Volatomic pattern of breast cancer and cancer-free tissues as a powerful strategy to identify potential biomarkers. Analyst 144 (2019), 4153–4161, 10.1039/c9an00263d.
Burklund, A., Saturley-Hall, H.K., Franchina, F.A., Hill, J.E., Zhang, John X.J., Printable QR code paper microfluidic colorimetric assay for screening volatile biomarkers. Biosens. Bioelectr. 128 (2019), 97–103, 10.1016/j.bios.2018.12.026.
Zanella, D., Henket, M., Schleich, F., Dejong, T., Louis, R., Focant, J.-F., Stefanuto, P.-H., Comparison of the effect of chemically and biologically induced inflammation on the volatile metabolite production of lung epithelial cells by GC×GC-TOFMS. Analyst, 2020, 10.1039/D0AN00720J.
Horváth, I., Barnes, P.J., Loukides, S., Sterk, P.J., Högman, M., Olin, A.C., Amann, A., Antus, B., Baraldi, E., Bikov, A., Boots, A.W., Bos, L.D., Brinkman, P., Bucca, C., Carpagnano, G.E., Corradi, M., Cristescu, S., De Jongste, J.C., Dinh-Xuan, A.T., Dompeling, E., Fens, N., Fowler, S., Hohlfeld, J.M., Holz, O., Jöbsis, Q., Van De Kant, K., Knobel, H.H., Kostikas, K., Lehtimäki, L., Lundberg, J.O., Montuschi, P., Van Muylem, A., Pennazza, G., Reinhold, P., Ricciardolo, F.L.M., Rosias, P., Santonico, M., van der Schee, M.P., van Schooten, F.J., Spanevello, A., Tonia, T., Vink, T.J., A european respiratory society technical standard: exhaled biomarkers in lung disease. Eur. Respir. J., 49, 2017, 10.1183/13993003.00965-2016.
Haick, H., Broza, Y.Y., Mochalski, P., Ruzsanyi, V., Amann, A., Assessment, origin, and implementation of breath volatile cancer markers. Chem. Soc. Rev. 43 (2014), 1423–1449, 10.1039/c3cs60329f.
Franchina, F.A., Mellors, T.R., Aliyeva, M., Wagner, J., Daphtary, N., Lundblad, L.K.A., Fortune, S.M., Rubin, E.J., Hill, J.E., Towards the use of breath for detecting mycobacterial infection: a case study in a murine model. J. Breath Res., 12, 2018, 10.1088/1752-7163/aaa016.
Mellors, T.R., Nasir, M., Franchina, F.A., Smolinska, A., Blanchet, L., Flynn, J.L., Tomko, J., O'Malley, M., Scanga, C.A., Lin, P.L., Wagner, J., Hill, J.E., Identification of Mycobacterium tuberculosis using volatile biomarkers in culture and exhaled breath. J. Breath Res., 13, 2019, 10.1088/1752-7163/aacd18 0–6.
Beccaria, M., Bobak, C., Maitshotlo, B., Mellors, T.R., Purcaro, G., Franchina, F.A., Rees, C.A., Nasir, M., Black, A., Hill, J.E., Exhaled human breath analysis in active pulmonary tuberculosis diagnostics by comprehensive gas chromatography-mass spectrometry and chemometric techniques. J. Breath Res., 13, 2019, 10.1088/1752-7163/aae80e.
Schleich, F.N., Zanella, D., Stefanuto, P.H., Bessonov, K., Smolinska, A., Dallinga, J.W., Henket, M., Paulus, V., Guissard, F., Graff, S., Moermans, C., Wouters, E.F.M., Van Steen, K., Van Schooten, F.J., Focant, J.F., Louis, R., Exhaled volatile organic compounds are able to discriminate between neutrophilic and eosinophilic asthma. Am. J. Respir. Crit. Care Med. 200 (2019), 444–453, 10.1164/rccm.201811-2210OC.
Singh, K.D., Tancev, G., Decrue, F., Usemann, J., Appenzeller, R., Barreiro, P., Jaumà, G., Macia Santiago, M., Vidal de Miguel, G., Frey, U., Sinues, P., Standardization procedures for real-time breath analysis by secondary electrospray ionization high-resolution mass spectrometry. Anal. Bioanal. Chem., 2019, 10.1007/s00216-019-01764-8.
Ligor, M., Ligor, T., Bajtarevic, A., Ager, C., Pienz, M., Klieber, M., Denz, H., Fiegl, M., Hilbe, W., Weiss, W., Lukas, P., Jamnig, H., Hackl, M., Buszewski, B., Miekisch, W., Schubert, J., Amann, A., Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry. Clin. Chem. Lab. Med. 47 (2009), 550–560, 10.1515/CCLM.2009.133.
Dettmer, K., Engewald, W., Adsorbent materials commonly used in air analysis for adsorptive enrichment and thermal desorption of volatile organic compounds. Anal. Bioanal. Chem. 373 (2002), 490–500, 10.1007/s00216-002-1352-5.
Woolfenden, E., Sorbent-based sampling methods for volatile and semi-volatile organic compounds in air. Part 1: sorbent-based air monitoring options. J. Chromatogr., A 1217 (2010), 2674–2684, 10.1016/j.chroma.2009.12.042.
Sterk, P.J., Modern inflammatory phenotyping of asthma breathomics is here to stay. Am. J. Respir. Crit. Care Med. 200 (2019), 405–406, 10.1164/rccm.201904-0733ED.
Amann, A., Miekisch, W., Pleil, J., Risby, T., Schubert, J., Methodological issues of sample collection and analysis of exhaled breath. Exhaled Biomarkers, 2010, 96–114, 10.1183/1025448x.00018509.
Pawliszyn, J., Lord, H.L., Handbook of Sample Preparation. 2011, 10.1002/9780813823621.
Amaral, M.S.S., Nolvachai, Y., Marriott, P.J., Comprehensive two-dimensional gas chromatography advances in technology and applications: biennial update. Anal. Chem. 92 (2020), 85–104, 10.1021/acs.analchem.9b05412.
Higgins Keppler, E.A., Jenkins, C.L., Davis, T.J., Bean, H.D., Advances in the application of comprehensive two-dimensional gas chromatography in metabolomics. TrAC Trends Anal. Chem. (Reference Ed.) 109 (2018), 275–286, 10.1016/j.trac.2018.10.015.
Harshman, S.W., Mani, N., Geier, B.A., Kwak, J., Shepard, P., Fan, M., Sudberry, G.L., Mayes, R.S., Ott, D.K., Martin, J.A., Grigsby, C.C., Storage stability of exhaled breath on Tenax TA. J. Breath Res., 10, 2016, 10.1088/1752-7155/10/4/046008.
Brown, V.M., Crump, D.R., Plant, N.T., Pengelly, I., Evaluation of the stability of a mixture of volatile organic compounds on sorbents for the determination of emissions from indoor materials and products using thermal desorption/gas chromatography/mass spectrometry. J. Chromatogr., A 1350 (2014), 1–9, 10.1016/j.chroma.2014.05.011.
Franchina, F.A., Purcaro, G., Burklund, A., Beccaria, M., Hill, J.E., Evaluation of different adsorbent materials for the untargeted and targeted bacterial VOC analysis using GC×GC-MS. Anal. Chim. Acta 1066 (2019), 146–153, 10.1016/j.aca.2019.03.027.
Berna, A.Z., Schaber, C.L., Bollinger, L.B., Mwale, M., Mlotha-Mitole, R., Trehan, I., Odom John, A.R., Comparison of breath sampling methods: a: post hoc analysis from observational cohort studies. Analyst 144 (2019), 2026–2033, 10.1039/c8an01823e.
Mansour, E., Vishinkin, R., Rihet, S., Saliba, W., Fish, F., Sarfati, P., Haick, H., Measurement of temperature and relative humidity in exhaled breath. Sensor. Actuator. B Chem., 304, 2020, 127371, 10.1016/j.snb.2019.127371.
Mochalski, P., King, J., Unterkofler, K., Amann, A., Stability of selected volatile breath constituents in Tedlar, Kynar and Flexfilm sampling bags. Analyst 138 (2013), 1405–1418, 10.1039/c2an36193k.
Ghimenti, S., Lomonaco, T., Bellagambi, F.G., Tabucchi, S., Onor, M., Trivella, M.G., Ceccarini, A., Fuoco, R., Di Francesco, F., Comparison of sampling bags for the analysis of volatile organic compounds in breath. J. Breath Res., 9, 2015, 10.1088/1752-7155/9/4/047110.
Henderson, B., Ruszkiewicz, D.M., Wilkinson, M., Beauchamp, J.D., Cristescu, S.M., Fowler, S.J., Salman, D., Di Francesco, F., Koppen, G., Langejuergen, J., Holz, O., Hadjithekli, A., Moreno, S., Pedrotti, M., Sinues, P., Slingers, G., Wilde, M., Lomonaco, T., Zanella, D., Zenobi, R., Focant, J.F., Grassin-Delyle, S., Franchina, F.A.A., Malásková, M., Stefanuto, P.-H., Pugliese, G., Mayhew, C.A., Thomas, C.L.P., A benchmarking protocol for breath analysis: the peppermint experiment. J. Breath Res., 2020, 10.1088/1752-7163/aba130.
Liberto, E., Ruosi, M.R., Cordero, C., Rubiolo, P., Bicchi, C., Sgorbini, B., Non-separative headspace solid phase microextraction-mass spectrometry profile as a marker to monitor coffee roasting degree. J. Agric. Food Chem. 61 (2013), 1652–1660, 10.1021/jf303067q.
Toledo, P.R.A.B., Pezza, L., Pezza, H.R., Toci, A.T., Relationship between the different aspects related to coffee quality and their volatile compounds. Compr. Rev. Food Sci. Food Saf. 15 (2016), 705–719, 10.1111/1541-4337.12205.