Article (Scientific journals)
Multiplex micro-SERS imaging of cancer-related markers in cells and tissues using poly(allylamine)-coated Au@Ag nanoprobes
Verdin, Alexandre; Malherbe, Cédric; Müller, Wendy et al.
2020In Analytical and Bioanalytical Chemistry, 412 (25)
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Keywords :
nanoparticles; molecular imaging; cancer; Raman; micro-SERS; multiplex; cells; tissues
Abstract :
[en] Surface-enhanced Raman scattering (SERS) nanoprobes based on Au@Ag core@shell nanoparticles coated with poly(allylamine) were functionalized with small targeting molecules to evaluate simultaneously the level of expression of two cancer-related markers, both in cells and in tissues. The Au@Ag nanoparticles provide a high SERS signal enhancement in the visible range when combined with resonant Raman-active molecules. The poly(allylamine) coating plays a dual key role in (i) protecting the metal surface against the complex biological medium, leading to a stable signal of the Raman-active molecules, and (ii) enabling specific biofunctionalization through its amine functions. Using small targeting molecules linked to the polymer coating, two different nanoprobes (duplex approach) were designed. Each was able to specifically target a particular cancer-related marker: folate receptors (FRs) and sialic acid (SA). We demonstrate that the level of expression of these targeted markers can be evaluated following the SERS signal of the probes incubated on cells or tissues. The potential overexpression of folate receptors and of sialic acid was evaluated and measured in breast and ovarian cancerous tissue sections. In addition, FR and/or SA overexpression in the tumor region can be visualized with high contrast with respect to the healthy region and with high spatial accuracy consistent with histology by SERS imaging of the nanoprobe signal. Owing to the unique spectral signature of the designed nanoprobes, this approach offers an efficient tool for the spatially resolved, in situ measurement of the expression level of several cancer-related markers in tumors at the same time.
Research center :
Mass Spectrometry Laboratory
Disciplines :
Chemistry
Author, co-author :
Verdin, Alexandre  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Malherbe, Cédric ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Müller, Wendy  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Bertrand, Virginie ;  Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences)
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Language :
English
Title :
Multiplex micro-SERS imaging of cancer-related markers in cells and tissues using poly(allylamine)-coated Au@Ag nanoprobes
Publication date :
10 September 2020
Journal title :
Analytical and Bioanalytical Chemistry
ISSN :
1618-2642
eISSN :
1618-2650
Publisher :
Springer, Germany
Volume :
412
Issue :
25
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 September 2020

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