[en] Objectives
The aim of this study was to assess the efficacy of lytic bacteriophages onStaphylococcus aureus causing bovine mastitis, by in vitro and in vivo assays using Galleria mellonella and murine mastitis models.
Methods
Between May and December 2016, tenS. aureus (five methicillin-resistant and five methicillin-sensitive) isolates were isolated from milk samples of cattle with mastitis in Belgium and Norway. The isolates were assessed in vitro for their susceptibility to four lytic bacteriophages (Romulus, Remus, ISP and DSM105264) and subsequently in vivo in G. mellonella larvae and in murine mastitis model.
Results
Romulus, Remus and ISP showed a lytic activity against theS. aureus isolates in vitro. A larvae survival rate below 50% was observed at four days post inoculation in the groups infected with a methicillin-sensitive S. aureus isolate and treated with these three phages in vivo. An incomplete recovery of the mice mastitis was observed at 48 hours post inoculation in the groups infected and treated with the ISP phage in vivo.
Conclusions
The observations are much more pronounced statistically between the infected-PBS treated and infected-phage treated groups inG. mellonella and murine mastitis model demonstrating an effect of the phages against S. aureus associated with bovine mastitis.
Duprez, Jean-Noël ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Département des maladies infectieuses et parasitaires (DMI)
Fergestad, M.; Norwegian University of Life Sciences > Department of Food Safety and Infection Biology
De Visscher, A.; Ghent University > Department of Reproduction, Obstetrics and Herd Health > M-team & Mastitis and Milk Quality Research Unit
L'Abee-Lund, T.; Norwegian University of Life Sciences > Department of Food Safety and Infection Biology
De Vliegher, S.; Ghent University > Department of Reproduction, Obstetrics and Herd Health > M-team & Mastitis and Milk Quality Research Unit
Wasteson, Y.; Norwegian University of Life Sciences > Department of Food Safety and Infection Biology
Touzain, F.; ANSES > Ploufragan-Plouzané laboratory > Viral Genetics and Bio-security Unit
Blanchard, Y.; ANSES > Ploufragan-Plouzané laboratory > Viral Genetics and Bio-security Unit,
Lavigne, R.; KU Leuven > Department of Biosystems > Laboratory of Gene Technology
Chanishvili, N.; Eliava Institute of Bacteriophages > R&D Department
Cassart, Dominique ; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Département de morphologie et pathologie (DMP)
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
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