Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrheic and septicemic calves and relatedness to human Shigatoxigenic E. coli O80:H2
Habets, Audrey; Crombé, F.; Nakamura, K.et al.
2021 • In Journal of Applied Microbiology, 130 (1), p. 258-264
[en] Aim: The purpose of this work was to identify and genetically characterize
enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli
(EPEC) O80:H2 from diarrhoeic and septicaemic calves in Belgium and to
comparing them with human EHEC after whole genome sequencing.
Methods and Results: Ten EHEC and 21 EPEC O80 identified by PCR
between 2009 and 2018 from faeces, intestinal content and a kidney of
diarrhoeic or septicaemic calves were genome sequenced and compared to 19
human EHEC identified between 2008 and 2019. They all belonged to the O80:
H2 serotype and ST301, harboured the eaeξ gene, and 23 of the 29 EHEC
contained the stx2d gene. Phylogenetically, they were distributed in two major
sub-lineages: one comprised a majority of bovine EPEC whereas the second
one comprised a majority of stx2d bovine and human EHEC.
Conclusions: Not only EPEC but also EHEC O80:H2 are present in diarrhoeic
and septicaemic calves in Belgium and are genetically related to human EHEC.
Significance and Impact of the Study: These findings support the need to
assess cattle as potential source of contamination of humans by EHEC O80:H2
and to understand the evolution of bovine and human EHEC and EPEC O80:
H2.
Crombé, F.; Vrije Universiteit Brussel > Belgian National Reference Center for STEC
Nakamura, K.; Kyushu University > Department of Bacteriology,
Guérin, Virginie ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
De Rauw, K.; Vrije Universiteit Brussel > Belgian National Reference Center for STEC
Piérard, D.; Vrije Universiteit Brussel > Belgian National Reference Center for STEC
Saulmont, M.; Association Régionale de Santé et d’Identification Animale (ARSIA)
Hayashi, T.; Kyushu University > Department of Bacteriology
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrheic and septicemic calves and relatedness to human Shigatoxigenic E. coli O80:H2
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