Methylmercury displays pro-adipogenic properties in rainbow trout preadipocytes

[en] Methylmercury (MeHg) is a ubiquitous contaminant largely found in aquatic environments, especially in species at high trophic level such as salmonids. The aim of this study was to evaluate the effects of MeHg on adipocyte differentiation and lipid metabolism in rainbow trout. Primary cultured preadipocytes were exposed to increasing concentrations of MeHg during six days with or without a hormonal cocktail. Main results showed a dose-dependent intracellular accumulation of neutral lipids with a preferential uptake of n-3 polyunsaturated fatty acids. Interestingly, this accumulation occurred after a fairly low uptake of MeHg by preadipocytes and was maintained after the cellular exposure to MeHg. In membrane phospholipids, arachidonic acid (20:4 n-6) was released in a dose-dependent manner. At the transcriptional level, the expression of several adipocyte-specific genes (perilipin 2 and apolipoprotein Eb) as well as lipid-related genes (fatty acid synthase and fatty acid binding protein 11a) was up-regulated in preadipocytes exposed to MeHg. These results highlight for the first time the disrupting effect of MeHg in trout adipocyte metabolism, providing new insights regarding the role of environmental pollutants in adipose tissue dysfunction and related pathologies.

Research center :

FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège

Disciplines :

Aquatic sciences & oceanology

Author, co-author :

Tinant, Gilles

Neefs, Ineke

Das, Krishna ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique

Rees, Jean-François

Larondelle, Yvan

Debier, Cathy

Language :

English

Title :

Methylmercury displays pro-adipogenic properties in rainbow trout preadipocytes

Publication date :

January 2021

Journal title :

Chemosphere

ISSN :

0045-6535

eISSN :

1879-1298

Publisher :

Elsevier, Oxford, United Kingdom

Volume :

263

Pages :

127917

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://doi.org/10.1016/j.chemosphere.2020.127917

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 08 September 2020

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