Development; Fish; Myosin heavy chain; Myosin light chain; Polyacrylamide gel electrophoresis; Tropomyosin; Troponin-C; Troponin-I; Troponin-T; Clariidae; Heterobranchus longifilis; Siluriformes
Abstract :
[en] Developmental changes in myofibrillar protein composition were investigated in the myotomal muscle of the African catfish, Heterobranchus longifilis (Clariidae), by several electrophoretic techniques. The main muscle fibres of larvae and the fast-white muscle fibres of juvenile and adult fish were found to express distinct myosin heavy chain and myosin light chain 2 (LC2) isoforms. Three myosin LC2 chains were successively detected, differing by their isoelectric points. In contrast, the alkali light chains remained qualitatively and quantitatively unchanged during fish growth. Actin, α-tropomyosin, and troponin-C (TN-C) were also similar in larval, juvenile, and adult white muscle, but an additional larval tropomyosin isoform was found in the first developmental stages. Two isoforms of troponin-T (TN-T) and troponin-I (TN-I) were synthesised in the course of fish growth. Transition from the larval to the adult isoform was much faster for TN-T than for TN-I. Slow-red muscle myofibrils from adult H. longifilis showed no common component (except actin) with larval, juvenile, or adult fast-white muscle myofibrils. Red myofibrils displayed a single TN-T and a single TN-I isoform, but two isoforms of TN-C. The myofibrillar protein isoforms synthesised at any given developmental stage almost certainly reflect changes in the functional requirements of swimming muscles in the course of fish development.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Huriaux, Françoise ; Université de Liège, Lab. de Biol. Cell. et Tissulaire, rue de Pitteurs 20, B-4020 Liège, Belgium
Vandewalle, Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Baras, Etienne; Université de Liège, Lab. Demographie Poissons A., B-4500 Tihange, Belgium
Legendre, Marc; IRD (ORSTOM), Grp. Aquacult. Continentale M., F-34033 Montpellier, France
Focant, Bruno W.W.; Université de Liège, Lab. de Biol. Cell. et Tissulaire, rue de Pitteurs 20, B-4020 Liège, Belgium
Language :
English
Title :
Myofibrillar proteins in white muscle of the developing African catfish Heterobranchus longifilis (Siluriforms, Clariidae)
Publication date :
December 1999
Journal title :
Fish Physiology and Biochemistry
ISSN :
0920-1742
eISSN :
1573-5168
Publisher :
Kluwer Academic Publishers, Netherlands
Volume :
21
Issue :
4
Pages :
287-301
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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