Barbus barbus; Development; Fish; Parvalbumin isotype; Polyacrylamide gel electrophoresis
Abstract :
[en] Parvalbumin isotypes PA II, PA III, PA IVa, and PA IVb were isolated by chromatography from trunk white muscle of barbel and physicochemical characterized. Electrospray ionization mass spectroscopy revealed that PA II has a lower molecular weight than the other isotypes and that PA IVa and PA IVb each consist of two subforms. Isotype distribution was studied by polyacrylamide gel electrophoresis. In adult fish, the total parvalbumin titre decreased and the isotype distribution varied from the anterior to the posterior myotomes. In the course of barbel development, the total parvalbumin titre increased rapidly as fish standard length increased from 1.3 to 5 cm; then sloped down gently as the length increased to 60 cm. At least six parvalbumin isotypes were identified, three of which are different forms (a, b, and c) of PA II. These three forms were present together at the larval stage, but PA IIc and chiefly PA IIb appeared as early isotypes, contrary to PA IIa which was present until the adult period. Later PA IVb accounted for up to 90% of the parvalbumin content; PA III and PA IVa are minor adult isotypes. Temporal and spatial variations in the total parvalbumin titre and in the differential expression of barbel parvalbumin isotypes very likely reflected the functional requirements of the fish axial musculature according to fish size and myotome location. Physiologically, the larval isotypes could promote faster relaxation of fast fibres than the adult isotypes, and hence favour shorter contraction times
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Huriaux, Françoise ; Université de Liège, Lab. de Biol. Cell. et Tissulaire, rue de Pitteurs 20, B-4020 Liège, Belgium
Collin, Serge; Université de Liège, Lab. de Biol. Cell. et Tissulaire, rue de Pitteurs 20, B-4020 Liège, Belgium
Vandewalle, Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Philippart, Jean-Claude ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie du comportement - Ethologie et psychologie animale
Focant, Bruno ; Université de Liège, Lab. de Biol. Cell. et Tissulaire, rue de Pitteurs 20, B-4020 Liège, Belgium
Language :
English
Title :
Characterization of parvalbumin isotypes in white muscle from the barbel and expression during development
Publication date :
April 1997
Journal title :
Journal of Fish Biology
ISSN :
0022-1112
eISSN :
1095-8649
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
50
Issue :
4
Pages :
821-836
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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