Deletion of bglC triggers a genetic compensation response by awakening the expression of alternative beta-glucosidase

[en] In the plant pathogen Streptomyces scabies, the gene bglC encodes a GH1 family cellobiose beta-glucosidase that is both required for primary metabolism and for inducing virulence of the bacterium. Deletion of bglC (strain ΔbglC) surprisingly resulted in the augmentation of the global beta-glucosidase activity of S. scabies. This paradoxical phenotype is highly robust as it has been observed in all bglC deletion mutants independently generated, thereby highlighting a phenomenon of genetic compensation. Comparative proteomics allowed to identify two glycosyl hydrolases – named BcpE1 and BcpE2 – of which peptide levels were significantly increased in strain ΔbglC. Quantitative RT-PCR revealed that the higher abundance of BcpE1 and BcpE2 is triggered at the transcriptional level, the expression of their respective gene being 100 and 15 times upregulated. Enzymatic studies with pure BcpE proteins showed that they both possess beta-glucosidase activity thereby explaining the genotypic-phenotypic discrepancy of the bglC deletion mutant. The GH1 family BcpE1 could hydrolyze cellobiose and generate glucose similarly to BglC itself thereby mainly contributing to the survival of strain ΔbglC when cellobiose is provided as sole nutrient source. The low affinity of BcpE2 for cellobiose suggests that this GH3 family beta-glucosidase would instead primarily target another and yet unknown glucose-beta-1,4-linked substrate. These results make S. scabies a new model system to study genetic compensation. Discovering how, either the bglC DNA locus, its mRNA, the BglC protein, or either its enzymatic activity controls bcpE genes' expression, will unveil new mechanisms directing transcriptional repression.

Research center :

CIP - Centre d'Ingénierie des Protéines - ULiège
InBios - Integrative Biological Sciences - ULiège

Disciplines :

Genetics & genetic processes

Author, co-author :

Deflandre, Benoit ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Thiébaut, Noémie ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Planckaert, Sören

Jourdan, Samuel

Anderssen, Sinaeda ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie

Hanikenne, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Devreese, Bart

Rigali, Sébastien ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Language :

English

Title :

Deletion of bglC triggers a genetic compensation response by awakening the expression of alternative beta-glucosidase

Publication date :

August 2020

Journal title :

Biochimica et Biophysica Acta. Gene Regulatory Mechanisms

ISSN :

1874-9399

eISSN :

1876-4320

Publisher :

Elsevier, Netherlands

Volume :

1863

Issue :

Peer reviewed :

Peer reviewed

Name of the research project :

PatScab#2

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 13 August 2020

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