Asparagine accumulation in chicory storage roots is controlled by translocation and feedback regulation of asparagine biosynthesis in leaves.

Soares, Emanoella; Shumbe, Léonard; Dauchot, Nicolas; Notté, Christine; Prouin, Claire; Maudoux, Olivier; Vanderschuren, Hervé

doi:10.1111/nph.16764

Article (Scientific journals)

Asparagine accumulation in chicory storage roots is controlled by translocation and feedback regulation of asparagine biosynthesis in leaves.

Soares, Emanoella; Shumbe, Léonard; Dauchot, Nicolas et al.

2020 • In New Phytologist, 228 (3), p. 922-931

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/250018

DOI
10.1111/nph.16764

PubMed
32729968

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Keywords :

asparagine; chicory; transport

Abstract :

[en] • The presence of acrylamide (AA), a potentially carcinogenic and neurotoxic compound, in food has become a major concern for public health. AA in plant-derived food mainly arises from the reaction of the amino acid asparagine (Asn) and reducing sugars during processing of foodstuffs at high temperature. • Using a selection of genotypes from the chicory (Cichorium intybus L.) germplasm we performed Asn measurements in storage roots and leaves to identify genotypes contrasting for Asn accumulation. We combined molecular analysis and grafting experiments to show that leaf to root translocation controls asparagine biosynthesis and accumulation in chicory storage roots. • We could demonstrate that Asn accumulation in storage roots depends on Asn biosynthesis and transport from the leaf, and that a negative feedback loop by Asn on CiASN1 expression impacts Asn biosynthesis in leaves. • Our results provide a new model for asparagine biosynthesis in root crop species and highlight the importance of characterizing and manipulating asparagine transport to reduce AA content in processed plant-based foodstuffs.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Soares, Emanoella

Shumbe, Léonard ; Université de Liège - ULiège > Département GxABT > Plant Sciences

Dauchot, Nicolas

Notté, Christine

Prouin, Claire

Maudoux, Olivier

Vanderschuren, Hervé ; Université de Liège - ULiège > Département GxABT > Plant Sciences

Language :

English

Title :

Asparagine accumulation in chicory storage roots is controlled by translocation and feedback regulation of asparagine biosynthesis in leaves.

Publication date :

05 July 2020

Journal title :

New Phytologist

ISSN :

0028-646X

eISSN :

1469-8137

Publisher :

Wiley, Oxford, United Kingdom

Volume :

228

Issue :

Pages :

922-931

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 09 August 2020

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