Article (Scientific journals)
Evaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs.
Vermout, Sandy M; Brouta, Frederic D; Descamps, Frederic F et al.
2004In FEMS Immunology and Medical Microbiology, 40 (1), p. 75-80
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Keywords :
Animals; Antibodies, Fungal/blood; Antigens, Fungal/immunology; Dermatomycoses/prevention & control; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Female; Fungal Vaccines/immunology; Guinea Pigs; Immunity, Cellular; Metalloproteases/immunology; Microsporum/enzymology/immunology; Recombinant Proteins/immunology; Treatment Outcome; Vaccination; Vaccines, Subunit/immunology
Abstract :
[en] In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected guinea pigs. Both humoral and cellular specific immune responses developing towards r-MEP3 were evaluated, by enzyme-linked immunosorbent assay and by in vitro lymphocyte transformation tests respectively. Vaccination induced a strong antibody response, and a significant but transient lymphoproliferative response against the protein. However, the protocol failed to prevent fungal invasion or development of dermatophytic lesions. These results show that under the present experimental conditions, r-MEP3 specific antibodies are not protective against a challenge exposure. They also suggest that in the same model, the induction of cell-mediated immunity towards r-MEP3 is not sufficient, indicating the need for further research in the field of specific immune mechanisms involved in M. canis dermatophytosis.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Vermout, Sandy M
Brouta, Frederic D
Descamps, Frederic F
Losson, Bertrand ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Mignon, Bernard ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Evaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs.
Publication date :
2004
Journal title :
FEMS Immunology and Medical Microbiology
ISSN :
0928-8244
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
40
Issue :
1
Pages :
75-80
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 October 2009

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