[en] In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected guinea pigs. Both humoral and cellular specific immune responses developing towards r-MEP3 were evaluated, by enzyme-linked immunosorbent assay and by in vitro lymphocyte transformation tests respectively. Vaccination induced a strong antibody response, and a significant but transient lymphoproliferative response against the protein. However, the protocol failed to prevent fungal invasion or development of dermatophytic lesions. These results show that under the present experimental conditions, r-MEP3 specific antibodies are not protective against a challenge exposure. They also suggest that in the same model, the induction of cell-mediated immunity towards r-MEP3 is not sufficient, indicating the need for further research in the field of specific immune mechanisms involved in M. canis dermatophytosis.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Vermout, Sandy M
Brouta, Frederic D
Descamps, Frederic F
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Evaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs.
Lunder M., Lunder M. Is Microsporum canis infection about to become a serious dermatological problem? Dermatology. 184:1992;87-89.
Gudding R., Lund A. Immunoprophylaxis of bovine dermatophytosis. Can. Vet. J. 36:1995;302-306.
Rybnikar A., Chumela J., Vrzal V., et al. Prophylactic and therapeutic use of a vaccine against trichophytosis in a large herd of silver foxes and arctic foxes. Acta Vet. Brno. 60:1991;285-288.
Gordon P.J., Bond R. Efficacy of a live attenuated Trichophyton verrucosum vaccine for control of bovine dermatophytosis. Vet. Rec. 139:1996;395-396.
Bratberg A.M., Solbakk I.T., Gyllensvaan C., et al. Efficacy of an inactivated and a live bovine ringworm vaccine in an experimental challenge study (abstract). Proc. Annu. Meet. Am. Acad. Vet. Dermatol. 15:1999;113-114.
Elad D., Segal E. Immunogenicity in calves of a crude ribosomal fraction of Trichophyton verrucosum: a field trial. Vaccine. 13:1995;83-87.
Elad D., Segal E. Immunogenicity in guinea pigs of a crude ribosomal fraction from Microsporum canis. Vaccine. 12:1994;134-138.
Pier A.C., Hodges A.B., Lauze J.M., Raisbeck M. Experimental immunity to Microsporum canis and cross reactions with other dermatophytes of veterinary importance. J. Med. Vet. Mycol. 33:1995;93-97.
Wawrzkiewicz K., Ziolkowska G. Specific immunoprophylaxis in Microsporum canis infection in guinea pigs. J. Mycol. Méd. 6:1996;56-62.
Bredahl L.K., Bratberg A.M., Solbakk I.T., et al. Efficacy of an experimental Microsporum canis vaccine in farmed foxes (abstract). Vet. Dermatol. 11:2000;39.
Deboer D.J., Moriello K.A. The immune response to Microsporum canis induced by a fungal cell wall vaccine. Vet. Dermatol. 5:1994;47-55.
Deboer D.J., Moriello K.A. Investigations of a killed dermatophyte cell wall vaccine against infection with Microsporum canis in cats. Res. Vet. Sci. 59:1995;110-113.
Deboer D.J., Moriello K.A., Blum J.L., Volk L.M., Bredahl L.K. Safety and immunologic effects after inoculation of inactivated and combined live-inactivated dermatophytosis vaccines in cats. Am. J. Vet. Res. 63:2000;1532-1537.
Fenner A., Karle J. Therapeutic vaccination against dermatophytosis in horses with Insol® Dermatophyton - results of a field-study in eleven German counties. Prakt. Tierarzt. 81:2000;574-578.
Brouta F., Descamps F., Fett T., Losson B., Gerday C., Mignon B. Purification and characterization of a 43.5 kDa keratinolytic metalloprotease from Microsporum canis. Med. Mycol. 39:2001;269-275.
Brouta F., Descamps F., Monod M., Vermout S., Losson B., Mignon B. Secreted metalloprotease gene family of Microsporum canis. Infect. Immun. 70:2002;5676-5683.
Brouta, F., Descamps, F., Monod, M., Vermout, S., Losson, B. and Mignon, B. (2003) Humoral and cell-mediated response to a recombinant keratinolytic metalloprotease (r-MEP3) from Microsporum canis in experimentally infected guinea pigs. Med. Mycol. (in press).
Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
Van Cutsem J. Animal models for dermatomycotic infections. Curr. Top. Med. Mycol. 3:1989;1-35.
Mignon B., Losson B. Prevalence and characterization of Microsporum canis carriage in cats. J. Med. Vet. Mycol. 35:1997;249-256.
Mignon B., Leclipteux T., Focant C., Nikkels A., Piérard G., Losson B. Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs. Med. Mycol. 37:1999;123-129.
Jones H.E., Reinhardt J.H., Rinaldi M.G. Acquired immunity to dermatophytes. Arch. Dermatol. 109:1974;840-848.
Deboer D.J., Moriello K.A. Humoral and cellular immune responses to Microsporum canis in naturally occurring feline dermatophytosis. J. Med. Vet. Mycol. 31:1993;121-132.
Sparkes A.H., Stokes C.R., Gruffydd-Jones T.J. Humoral immune responses in cats with dermatophytosis. Am. J. Vet. Res. 54:1993;1869-1873.
Casadevall A. Antibody immunity and invasive fungal infections. Infect. Immun. 63:1995;4211-4218.
Casadevall A., Feldmesser M., Pirofski L. Induced humoral immunity and vaccination against major human fungal pathogens. Curr. Opin. Microbiol. 5:2002;386-391.
Bromuro C., Torosantucci A., Chiani P., Conti S., Polonelli L., Cassone A. Interplay between protective and inhibitory antibodies dictates the outcome of experimentally disseminated Candidiasis in recipients of a Candida albicans vaccine. Infect. Immun. 70:2002;5462-5470.
Woodfolk J.A., Slunt J.B., Deuell B., Hayden M.L., Platts-Mills T.A. Definition of a Trichophyton protein associated with delayed hypersensitivity in humans. Evidence for immediate (IgE and IgG4) and delayed hypersensitivity to a single protein. J. Immunol. 156:1996;1695-1701.
Sudowe S., Arps V., Vogel T., Kolsch E. The role of interleukin-4 in the regulation of sequential isotype switch from immunoglobulin G1 to immunoglobulin E antibody production. Scand. J. Immunol. 51:2000;461-471.
Calderon R.A., Hay R.J. Cell-mediated immunity in experimental murine dermatophytosis II. Adoptive transfer of immunity to dermatophyte infection by lymphoid cells from donors with acute or chronic infections. Immunology. 53:1984;465-472.
Deboer D.J., Moriello K.A., Cooley A.J. Immunological reactivity to intradermal dermatophyte antigens in cats with dermatophytoses. Vet. Dermatol. 2:1991;59-67.
Sparkes A.H., Stokes C.R., Gruffydd-Jones T.J. Experimental Microsporum canis infection in cats: correlation between immunological and clinical observations. J. Med. Vet. Mycol. 33:1995;177-184.
Kerbs S., Greenberg J., Jesrani K. Temporal correlation of lymphocyte blastogenesis, skin test responses, and erythema during dermatophyte infections. Clin. Exp. Immunol. 27:1977;526-530.
Hay, R.J. (1997) Fungal infections. In: Fungal Disease. Biology, Immunology and Diagnosis (Jacobs, P.H. and Nall, L., Eds.), pp. 209-218. Marcel Dekker, New York.
Ogawa H., Summerbell R.C., Clemons K.V., Koga T., Ran Y.P., Rashid A., Sohnle P.G., Stevens D.A., Tsuboi R. Dermatophytes and host defence in cutaneous mycoses. Med. Mycol. 36:1998;166-173.
Slunt J.B., Taketomi E.A., Woodfolk J.A., Hayden M.L., Platts-Mills T.A. The immune response to Trichophyton tonsurans: distinct T cell cytokine profiles to a single protein among subjects with immediate and delayed hypersensitivity. J. Immunol. 157:1996;5192-5197.
Rudd P.M., Elliott T., Cresswell P., Wilson I.A., Dwek R.A. Glycosylation and the immune system. Science. 291:2001;2370-2376.
Bromuro C., La Valle R., Sandini S. A 70-kilodalton recombinant heat shock protein of Candida albicans is highly immunogenic and enhances systemic murine candidiasis. Infect. Immun. 66:1998;2154-2162.
Comoy E.E., Capron A., Typhronitis G. In vivo induction of type 1 and 2 immune responses against protein antigens. Int. Immunol. 9:1997;523-531.
Moingeon P., Haensler J., Lindberg A. Towards the rational design of Th1 adjuvants. Vaccine. 19:2001;4363-4372.