Article (Scientific journals)
Analysis of DNA methylation patterns associated with drought stress response in faba bean (Vicia faba L.) using methylation-sensitive amplification polymorphism (MSAP)
Abid, G.; Mingeot, D.; Muhovski, Y. et al.
2017In Environmental and Experimental Botany, 142, p. 34-44
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Keywords :
DNA methylation; Drought stress; Faba bean; Gene expression; Methylation-sensitive amplification polymorphism (MSAP); Vicia faba
Abstract :
[en] The effects of drought on water relations, gas exchanges and epigenetic alterations were studied in two faba bean (Vicia faba L.) genotypes with contrasting levels of drought tolerance. The drought-tolerant Bachar genotype was less affected by water deficit in comparison with the drought-sensitive F177 in terms of photosynthetic function and water status in plants as indicated by less reduction in net photosynthesis (A), transpiration rate (E), stomatal conductance (gs) and relative water content (RWC). In this study, the methylation-sensitive amplification polymorphism (MSAP) technique was used to profile the DNA methylation patterns of Bachar and F177 under drought and control conditions. Overall, the amount of methylation was higher in leaves than in roots and the contribution of fully methylated loci was always higher than that of hemimethylated loci. Under control and drought treatment, the total methylation level in leaf tissues was 37.43% and 30.62% in Bachar, 41.23% and 38.16% in F177, indicating a decrease of 6.81% and 3.07% in Bachar and F177 respectively, due to drought exposure. The results revealed that DNA methylation in root tissues was decreased by 3.63% (from 23.43% to 19.80%) in Bachar and increased by 0.66% (from 16.53% to 17.19%) in F177 under drought stress. Differentially displayed DNA fragments in MSAP profiles were cloned and sequenced. A sequence analysis identified six potentially drought stress-related differentially methylated regions (DMRs). Five of these have high homology to previously identified or putative proteins found in other plants, including lipoxygenase (LOX), calcium-dependent protein kinase (CDPK), ABC transporter family (ABC), glycosyl hydrolase (GH) and phosphoenolpyruvate carboxylase (PEPC) and were chosen for further characterization. Quantitative reverse transcription PCR analysis revealed that VfLOX, VfCDPK, VfABC and VfGH varied under drought stress; the expression level of these studied genes was higher in Bachar than in F177. This could suggest their possible role in faba bean drought stress tolerance. Overall, the genome-wide epigenetic changes are probably an important regulatory mechanism for faba bean response to drought and as well to other environmental stresses. © 2017 Elsevier B.V.
Disciplines :
Agriculture & agronomy
Author, co-author :
Abid, G.;  University of Tunis El Manar, Center of Biotechnology of Borj Cedria, Laboratory of Legumes, 901, Hammam-LifB-2050, Tunisia
Mingeot, D.;  Department of Life Sciences, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, Gembloux, B-5030, Belgium
Muhovski, Y.;  Department of Life Sciences, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, Gembloux, B-5030, Belgium
Mergeai, Guy ;  Université de Liège - ULiège > Département GxABT > Plant Sciences
Aouida, M.;  University of Tunis El Manar, Center of Biotechnology of Borj Cedria, Laboratory of Legumes, 901, Hammam-LifB-2050, Tunisia
Abdelkarim, S.;  University of Tunis El Manar, Center of Biotechnology of Borj Cedria, Laboratory of Legumes, 901, Hammam-LifB-2050, Tunisia
Aroua, I.;  University of Tunis El Manar, Center of Biotechnology of Borj Cedria, Laboratory of Legumes, 901, Hammam-LifB-2050, Tunisia
El Ayed, M.;  University of Tunis El Manar, Center of Biotechnology of Borj Cedria, Laboratory of Bioactive Substances, 901, Hammam-LifB-2050, Tunisia
M'hamdi, M.;  Higher Agronomic Institute of Chott Mariem, BP 47, Chott Mariem, Sousse, 4042, Tunisia
Sassi, K.;  Laboratory of Agronomy, Department of Agronomy and Plant Biotechnology, National Agronomy Institute of Tunisia (INAT), University of Carthage, Avenue Charles Nicolle, 43, Tunis-Mahrajène, 1082, Tunisia
Jebara, M.;  University of Tunis El Manar, Center of Biotechnology of Borj Cedria, Laboratory of Legumes, 901, Hammam-LifB-2050, Tunisia
Language :
English
Title :
Analysis of DNA methylation patterns associated with drought stress response in faba bean (Vicia faba L.) using methylation-sensitive amplification polymorphism (MSAP)
Publication date :
2017
Journal title :
Environmental and Experimental Botany
ISSN :
0098-8472
eISSN :
1873-7307
Publisher :
Elsevier B.V.
Volume :
142
Pages :
34-44
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 August 2020

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