Isoform distribution of parvalbumins and of some myofibrillar proteins in adult and developing Chrysichthys auratus (Geoffroy st. Hilaire, 1808) (pisces, claroteidae)
Chikou, Antoine; Huriaux, Françoise; Lalèyé, Philippe Adédjobiet al.
1997 • In Archives of Physiology and Biochemistry, 105 (6), p. 611-617
[en] Polyacrylamide gel electrophoresis was used to analyse the distribution of parvalbumin, myosin light chain, and troponin 1 isoforms in white muscles of larval, juvenile, and adult Chrysichthys auratus (catfish, siluriforms) and to study the kinetics of their synthesis. Parvalbumin isoform PA II was first detected from day 5 post-hatching and was the main 'larval' isoform in this species. PA III appeared at the beginning of the juvenile stage but always remained the minor isoform, even in adult fish. Young mature specimens (approximately 12 cm long) displayed the highest total parvalbumin content. Adult-type myosin light chains were detected from day 8. Densitometric analysis confirmed the light-chain distribution typical of fish muscles, with a relatively high amount of LC3 and a low amount of LC1. We evidenced a 'larval' form of troponin-I and its progressive replacement by an 'adult' form.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Chikou, Antoine; Univ. Natl. du Bénin, Fac. des Sci. Agronomiques, Lab. d'Hydrobiologie et d'Aquacult., Abomey-Calavi, Cotonou, Benin
Huriaux, Françoise ; Université de Liège, Institut d'Anatomie, Lab. de Biol. Cell. et Tissulaire, Liège, Belgium
Lalèyé, Philippe Adédjobi
Vandewalle, Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Focant, Bruno ; Université de Liège, Institut d'Anatomie, Lab. de Biol. Cell. et Tissulaire, Liège, Belgium, Université de Liège, Institut d'Anatomie, Lab. de Biol. Cell. et Tissulaire, rue de Pitteurs, 20, B-4020 Liège, Belgium
Language :
English
Title :
Isoform distribution of parvalbumins and of some myofibrillar proteins in adult and developing Chrysichthys auratus (Geoffroy st. Hilaire, 1808) (pisces, claroteidae)
Publication date :
1997
Journal title :
Archives of Physiology and Biochemistry
ISSN :
1381-3455
eISSN :
1744-4160
Publisher :
Swets and Zeitlinger, Netherlands
Volume :
105
Issue :
6
Pages :
611-617
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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