Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples
[en] A simple and sensitive method to quantify fve diferent arabidopsides by HPLC—ion trap mass
spectrometry in complex plant samples was developed and validated. Arabidopsides are oxidized
galactolipids frst described in Arabidopsis thaliana but also produced by other plant species under
stress conditions. External calibration was performed using arabidopsides purifed from reezethawed Arabidopsis leaves. Lipids were extracted and pre-purifed on an SPE silica column before HPLC–MS analysis.Arabidopsides were separated on a C18 column using a gradient of mQ water and acetonitrile:mQ water (85:15) supplemented with formic acid (0.2%) and ammonium formate (12 mM).The method was validated according to European commission decision 2002/657/CE. LOD, LOQ, linearity, intra-day and inter-day precision and accuracy, selectivity, matrix efects and recoveries were determined for the fve metabolites.The established method is highly selective in a complex plant matrix. LOD and LOQ were, respectively, in the range 0.098–0.78 and 0.64–1.56 µM, allowing the arabidopside quantifcation from 25.6–62.4 nmol/g fresh weight. Calibration curve correlation coefcients were higher than 0.997. Matrix efects ranged from -2.09% to 6.10% and recoveries between 70.7% and 109%.The method was successfully applied to complex plant matrixes: Arabidopsis thaliana and Nasturtium officinale.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Genva, Manon ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Andersson, Mats X
Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Language :
English
Title :
Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples
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