Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples

Genva, Manon; Andersson, Mats X; Fauconnier, Marie-Laure

doi:10.1038/s41598-020-68757-x

Article (Scientific journals)

Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples

Genva, Manon; Andersson, Mats X; Fauconnier, Marie-Laure

2020 • In Scientific Reports, 10, p. 11957

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/249729

DOI
10.1038/s41598-020-68757-x

PubMed
32686714

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Keywords :

oxylipin; arabidopside; LC-MS

Abstract :

[en] A simple and sensitive method to quantify fve diferent arabidopsides by HPLC—ion trap mass spectrometry in complex plant samples was developed and validated. Arabidopsides are oxidized galactolipids frst described in Arabidopsis thaliana but also produced by other plant species under stress conditions. External calibration was performed using arabidopsides purifed from reezethawed Arabidopsis leaves. Lipids were extracted and pre-purifed on an SPE silica column before HPLC–MS analysis.Arabidopsides were separated on a C18 column using a gradient of mQ water and acetonitrile:mQ water (85:15) supplemented with formic acid (0.2%) and ammonium formate (12 mM).The method was validated according to European commission decision 2002/657/CE. LOD, LOQ, linearity, intra-day and inter-day precision and accuracy, selectivity, matrix efects and recoveries were determined for the fve metabolites.The established method is highly selective in a complex plant matrix. LOD and LOQ were, respectively, in the range 0.098–0.78 and 0.64–1.56 µM, allowing the arabidopside quantifcation from 25.6–62.4 nmol/g fresh weight. Calibration curve correlation coefcients were higher than 0.997. Matrix efects ranged from -2.09% to 6.10% and recoveries between 70.7% and 109%.The method was successfully applied to complex plant matrixes: Arabidopsis thaliana and Nasturtium officinale.

Disciplines :

Agriculture & agronomy
Chemistry

Author, co-author :

Genva, Manon ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes

Andersson, Mats X

Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes

Language :

English

Title :

Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples

Publication date :

20 July 2020

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Nature Publishing Group, London, United Kingdom

Volume :

Pages :

art.11957

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.nature.com/articles/s41598-020-68757-x

Name of the research project :

Grant number 2016/V 3/5/141-IB/JN-3049

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 22 July 2020

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