Differential fungicidal activities of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodology.

Meletiadis, Joseph; Antachopoulos, Charalampos; STERGIOPOULOU, Theodouli; Pournaras, Spyros; Roilides, Emmanuel; Walsh, Thomas J.

doi:10.1128/AAC.00345-07

Article (Scientific journals)

Differential fungicidal activities of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodology.

Meletiadis, Joseph; Antachopoulos, Charalampos; STERGIOPOULOU, Theodouli et al.

2007 • In Antimicrobial Agents and Chemotherapy, 51 (9), p. 3329-37

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/249631

DOI
10.1128/AAC.00345-07

PubMed
17576838

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Keywords :

Amphotericin B/pharmacology; Antifungal Agents/pharmacology; Aspergillus/drug effects/growth & development; Colony Count, Microbial; Colorimetry; Culture Media; Microbial Sensitivity Tests/methods; Pyrimidines/pharmacology; Quality Control; Regression Analysis; Reproducibility of Results; Species Specificity; Spores, Fungal/drug effects; Tetrazolium Salts; Triazoles/pharmacology; Vitamin K 3/pharmacology

Abstract :

[en] Antifungal agents may differ in their fungicidal activities against Aspergillus spp. In order to compare the fungicidal activities of voriconazole and amphotericin B against 40 isolates of Aspergillus fumigatus, A. flavus, and A. terreus, we developed a new microbroth colorimetric method for assessing fungicidal activities and determining minimal fungicidal concentrations (MFCs). This methodology follows the antifungal susceptibility testing reference method M-38A for MIC determination. After drug removal and addition of fresh medium, growth of viable conidia adhering to the bottoms of the microtitration wells was assessed by a colorimetric assay of metabolic activity after 24 h of incubation. The new method was faster (six times), reproducible (92 to 97%), and in agreement with culture-based MFCs (91 to 100%). Differential fungicidal activities of voriconazole and amphotericin B were found among the three Aspergillus species, with A. fumigatus and A. flavus having the lowest (1 and 2 mg/liter, respectively) and A. terreus the highest (>16 mg/liter) median amphotericin B MFCs; A. flavus had a lower median voriconazole MFC (4 mg/liter) than the other species (>8 mg/liter; P < 0.05). Amphotericin B was fungicidal (MFC/MIC </= 4) against all A. fumigatus and A. flavus isolates but no A. terreus isolates, whereas voriconazole was fungicidal against 82% of A. flavus isolates and fungistatic (MFC/MIC > 4) against 94% of A. fumigatus and 84% of A. terreus isolates. The new methodology revealed a concentration-dependent sigmoid pattern of fungicidal effects, indicating that fungicidal activity is not an all-or-nothing phenomenon and that some degree of fungicidal action can be found even for agents considered fungistatic based on the MFC/MIC ratio.

Disciplines :

Immunology & infectious disease

Author, co-author :

Meletiadis, Joseph

Antachopoulos, Charalampos

STERGIOPOULOU, Theodouli ; Centre Hospitalier Universitaire de Liège - CHU > Pédiatrie

Pournaras, Spyros

Roilides, Emmanuel

Walsh, Thomas J.

Language :

English

Title :

Differential fungicidal activities of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodology.

Publication date :

2007

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology, United States - District of Columbia

Volume :

Issue :

Pages :

3329-37

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 July 2020

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