[en] Dermatomycoses are among the world's most common diseases and their incidence has increased over recent years, particularly in immunosuppressed patients. In previous studies, the saponin CAY-1 from cayenne pepper (Capsicum frutescens), has shown antifungal activities against Candida albicans and Aspergillus spp. We therefore studied the in vitro antifungal activity of CAY-1 against non-germinating conidia and hyphae of clinical isolates of the dermatophytes Trichophyton mentagrophytes, T. rubrum, T. tonsurans and Microsporum canis. We used a microdilution method to assess the growth inhibitory activities of CAY-1 against conidia (CLSI document M38-A) and a colorimetric procedure (XTT method) to investigate the metabolic inhibitory activity of CAY-1 against hyphae. The minimal inhibitory concentrations (complete visual growth inhibition) of CAY-1 against non-germinating conidia ranged from 10-20 microg/ml for all dermatophyte isolates included in this investigation. In addition, we found >90% inhibition of hyphal metabolic activity of these same isolates with 10-20 microg/ml of CAY-1. Results indicate that CAY-1 merits further investigation as a potential agent for the treatment of dermatomycoses.
Borgers, M., Degreef, H. and Cauwenbergh, G. (2005) Fungal infections of the skin: Infection process and antimycotic therapy. Curr Drug Targets, 6, pp. 849-862.
Mays, SR, Bogle, MA and Bodey, GP. (2006) Cutaneous fungal infections in the oncology patient: Recognition and management. Am J Clin Dermatol, 7, pp. 31-43.
Hainer, BL. (2003) Dermatophyte infections. Am Fam Physician, 67, pp. 101-108.
Weitzman, I. and Summerbell, RC. (1995) The dermatophytes. Clin Microbiol Rev, 8, pp. 240-259.
Ali-Shtayeh, MS and Abu Ghdeib, SI. (1999) Antifungal activity of plant extracts against dermatophytes. Mycoses, 42, pp. 665-672.
Hammer, KA, Carson, CF and Riley, TV. (1999) Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol, 86, pp. 985-990.
De Lucca, AJ, Bland, JM Boue, S. et al. (2006) Synergism of CAY-1 with amphotericin B and itraconazole. Chemotherapy, 52, pp. 285-287.
Renault, S., De Lucca, AJ Boue, S. et al. (2003) CAY-1, a novel antifungal compound from cayenne pepper. Med Mycol, 41, pp. 75-81.
De Lucca, AJ, Bland, JM Vigo, CB et al. (2002) CAY-I, a fungicidal saponin from Capsicum sp. fruit. Med Mycol, 40, pp. 131-137.
NCCLS (2002) Reference Method for Both Dilution Antifungal Susceptibility Testing of Filamentous Fungi. 22:16
Sanguinetti, M., Posteraro, B. Romano, L. et al. (2007) In vitro activity of Citrus bergamia (bergamot) oil against clinical isolates of dermatophytes. J Antimicrob Chemother, 59, pp. 305-308.
De Lucca, AJ, Bland, JM Vigo, CB et al. (2000) D-cecropin B: Proteolytic resistance, lethality for pathogenic fungi and binding properties. Med Mycol, 38, pp. 301-308.
Vincken, JP, Heng, L., de Groot, A. and Gruppen, H. (2007) Saponins, classification and occurrence in the plant kingdom. Phytochemistry, 68, pp. 275-297.
Zhang, XF, Cui, Y. Huang, JJ et al. (2007) Immuno-stimulating properties of diosgenyl saponins isolated from Paris polyphylla. Bioorg Med Chem Lett, 17, pp. 2408-2413.
Gurfinkel, DM and Rao, AV. (2003) Soyasaponins: The relationship between chemical structure and colon anticarcinogenic activity. Nutr Cancer, 47, pp. 24-33.
Kim, SW, Park, SK Kang, SI et al. (2003) Hypocholesterolemic property of Yucca schidigera and Quillaja saponaria extracts in human body. Arch Pharm Res, 26, pp. 1042-1046.
Guclu-Ustundag, O. and Mazza, G. (2007) Saponins: Properties, applications and processing. Crit Rev Food Sci Nutr, 47, pp. 231-258.
De Lucca, AJ, Boue, S., Palmgren, MS, Maskos, K. and Cleveland, TE. (2006) Fungicidal properties of two saponins from Capsicum frutescens and the relationship of structure and fungicidal activity. Can J Microbiol, 52, pp. 336-342.
Martin, KW and Ernst, E. (2004) Herbal medicines for treatment of fungal infections: A systematic review of controlled clinical trials. Mycoses, 47, pp. 87-92.
