Reference : Carrier-induced, hapten-specific suppression: a problem of antigen presentation?
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/249069
Carrier-induced, hapten-specific suppression: a problem of antigen presentation?
English
Renjifo, X. [Université Libre de Bruxelles - ULB > Biologie Moléculaire > > >]
Wolf, S. [Genetics Institute > > > >]
Pastoret, Paul-Pierre mailto [Université de Liège - ULiège > Médecine vétérinaire > Virologie-Immunologie > >]
Bazin, H. [Université Catholique de Louvain - UCL > Unité d’Immunologie Expérimentale > > >]
Urbain, J. [Université Libre de Bruxelles - ULB > Biologie Moléculaire > > >]
Leo, O. [Université Libre de Bruxelles - ULB > Biologie Moléculaire > > >]
Moser, M. [Université Libre de Bruxelles - ULB > Biologie Moléculaire > > >]
1998
Journal of Immunology
American Association of Immunologists
161
2
702-6
Yes (verified by ORBi)
0022-1767
1550-6606
MD
[en] Animals ; Antigen Presentation ; Antigens, T-Independent/administration & dosage/immunology/metabolism ; Carrier Proteins/administration & dosage/immunology ; Dendritic Cells/immunology/metabolism ; Epitopes/administration & dosage/immunology/metabolism ; Female ; Haptens/administration & dosage/immunology/metabolism ; Hemocyanin/administration & dosage/immunology ; Immunization ; Immunoglobulin G/biosynthesis ; Immunosuppression ; Injections, Intraperitoneal ; Interleukin-12/physiology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred DBA ; Picrates/administration & dosage/immunology
[en] Prior immunity against a carrier protein has been shown to modulate the serologic response to injected haptens attached to the same carrier. In particular, a carrier/hapten-carrier immunization protocol induces marked suppression for IgG2a anti-hapten Ab production but does not interfere with anti-carrier Ab responses. Although the phenomenon of epitopic suppression has been amply demonstrated, the mechanism underlying the suppression remains unknown. The selective deficiency in IgG2a secretion suggests that IFN-gamma-producing Th1 cells are not properly activated. We and others have shown that the nature of the APCs present during the first encounter with the Ag influences the development of selected Th populations in vivo; dendritic cells (DCs) seem to be required for the induction of primary, Th1-type responses. Since carrier priming induces the clonal expansion of specific B cells that appear to efficiently capture the Ag, we hypothesized that the hapten-carrier conjugate may be presented by B cells in preimmunized animals. Therefore, we immunized mice to the conjugate by injecting syngeneic DCs pulsed in vitro with the Ag. Our data show that an injection of DCs and IL-12 prevents epitopic suppression, suggesting that it may result from defective Ag presentation.
http://hdl.handle.net/2268/249069

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
ORBiCarrier.pdfAuthor postprint340.77 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.