Structure-Function Elucidation of a New α-Conotoxin, MilIA, from Conus milneedwardsi

Cone snail toxins; Conopeptide; Drug development; Electrophysiology; Ion channel diseases; Nicotinic acetylcholine receptor; Article; Conus (snail); Conus milneedwardsi; Xenopus laevis; Amino Acid Sequence; Animals; Conotoxins; Conus Snail; Mutation; Neurotoxins; Nicotinic Antagonists; Oocytes; Patch-Clamp Techniques; Peptides; Receptors, Nicotinic; Recombinant Proteins; Structure-Activity Relationship

Abstract :

[en] The a-Conotoxins are peptide toxins that are found in the venom of marine cone snails and they are potent antagonists of various subtypes of nicotinic acetylcholine receptors (nAChRs). Because nAChRs have an important role in regulating transmitter release, cell excitability, and neuronal integration, nAChR dysfunctions have been implicated in a variety of severe pathologies. We describe the isolation and characterization of α-conotoxin MilIA, the first conopeptide from the venom of Conus milneedwardsi. The peptide was characterized by electrophysiological screening against several types of cloned nAChRs that were expressed in Xenopus laevis oocytes. MilIA, which is a member of the α3/5 family, is an antagonist of muscle type nAChRs with a high selectivity for muscle versus neuronal subtype nAChRs. Several analogues were designed and investigated for their activity in order to determine the key epitopes of MilIA. Native MilIA and analogues both showed activity at the fetal muscle type nAChR. Two single mutations (Met9 and Asn10) allowed for MilIA to strongly discriminate between the two types of muscle nAChRs. Moreover, one analogue, MilIA [∆1,M2R, M9G, N10K, H11K], displayed a remarkable enhanced potency when compared to native peptide. The key residues that are responsible for switching between muscle and neuronal nAChRs preference were elucidated. Interestingly, the same analogue showed a preference for α9α10 nAChRs among the neuronal types. © 2019 by the authors.

Disciplines :

Chemistry

Author, co-author :

Peigneur, S.; Toxicology and Pharmacology, KU Leuven, Campus Gasthuisberg, O & N2, Herestraat 49, P.O. Box 922, Leuven, 3000, Belgium

Devi, P.; Toxicology and Pharmacology, KU Leuven, Campus Gasthuisberg, O & N2, Herestraat 49, P.O. Box 922, Leuven, 3000, Belgium, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403 004, India

Seldeslachts, A.; Toxicology and Pharmacology, KU Leuven, Campus Gasthuisberg, O & N2, Herestraat 49, P.O. Box 922, Leuven, 3000, Belgium

Ravichandran, S.; Center of Advanced Study in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu 608 502, India

Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique

Tytgat, J.; Toxicology and Pharmacology, KU Leuven, Campus Gasthuisberg, O & N2, Herestraat 49, P.O. Box 922, Leuven, 3000, Belgium

Language :

English

Title :

Structure-Function Elucidation of a New α-Conotoxin, MilIA, from Conus milneedwardsi

Publication date :

2019

Journal title :

Marine Drugs

ISSN :

1660-3397

Publisher :

MDPI AG

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

PDM/19/164CELSA/17/047EMBO: ESTF7241EMBO

Funders :

KU Leuven - Katholieke Universiteit Leuven

Available on ORBi :

since 24 June 2020

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