Unpublished conference/Abstract (Scientific congresses and symposiums)
Impact of mesenchymal stromal cells and/or everolimus on T-reg lymphocyte expansion in rats
Vandermeulen, Morgan; ERPICUM, Pauline; POMA, Laurence et al.
2020Annual Meeting
 

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Keywords :
organ transplantation; transplantation; tolerance; research; MSC; stem cell
Abstract :
[en] Background: Mesenchymal stromal cells (MSC) are a promising cell-therapy in solid-organ transplantation, namely because of their immunomodulatory properties and positive impact on the expansion of T-regulator lymphocytes (Treg). The “optimal” immunosuppressive regimen to be associated with MSC has not been defined. Here, we aimed to evaluate the effects on Treg expansion of a single injection of MSC combined or not with everolimus in rats. Materials and methods: Twenty-four Lewis rats were randomly assigned to 4 groups (n=6 per group): MSC+Evero group, i.e. everolimus (0,25mg/kg/day, SC) from D0 to D14 and iv MSC (±1x106 cells) at D9; MSC group, i.e. placebo from D0 to D14 and iv MSC at D9; Evero group, i.e. everolimus from D0 to D14 and iv saline at D9; control group, i.e. placebo from D0 to D14 and iv saline at D9. T-reg blood levels were measured at D0-14-28 with flow cytometry analysis using anti-CD4,-CD25 and -FoxP3 antibodies. Results: In the two groups infused with MSC, Treg were significantly expanded at D14 and D28 (p<0.01), in comparison to D0. When compared to controls group, the “Evero” group showed a significant expansion of Treg levels at D14 but not at D28. In control, Treg levels did not significantly change compared to D0. Conclusion: A single iv MSC injection was efficient to expand T-reg blood levels. This effect was not altered by everolimus co-administration. Everolimus exposure alone promotes a transient T-reg expansion. Hence, everolimus may be regarded as a co-drug of choice in MSC-based therapy in solid-organ transplantion.
Disciplines :
Gastroenterology & hepatology
Surgery
Author, co-author :
Vandermeulen, Morgan ;  Université de Liège - ULiège > Département des sciences cliniques > Pathologie chirurgicale abdominale et endoctrinienne
ERPICUM, Pauline ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
POMA, Laurence ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
Detry, Olivier  ;  Université de Liège - ULiège > Département des sciences cliniques > Pathologie chirurgicale abdominale et endocrinienne
JOURET, François  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
Language :
English
Title :
Impact of mesenchymal stromal cells and/or everolimus on T-reg lymphocyte expansion in rats
Publication date :
11 March 2020
Event name :
Annual Meeting
Event organizer :
Belgian Transplantation Society
Event place :
Antwerp, Belgium
Event date :
le 11 mars 2020
Available on ORBi :
since 22 June 2020

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