Cichlids; Fish systematics; Paralbumins; Polyacrylamide gel electrophoresis; Animal; Electrophoresis, Polyacrylamide Gel; Muscles; Parvalbumins; Perches; Species Specificity
Abstract :
[en] This paper shows the usefulness of polyacrylamide gel electrophoresis (PAGE) separation of a few well-characterized muscle proteins, the paralbumins, in the systematic study of 13 cichlid species. The separation of the isoforms of these abundant, quite species-specific fish paralbumins is fast, easy, and requires but small quantities of muscle material. Used alone, this technique suggests hypotheses for species classification. In conjunction with morphological analysis, it makes it possible to confirm or invalidate doubts about the determination.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Focant, Bruno ; Laboratoire de Biologie cellulaire et tissuloire, Université de Liège, Institut ďAnatomie, 20, rue de Pitteurs, B-4020, Liège, Belgium
Lalèyé, Philippe Adédjobi; University of Abomey-Calavi, Cotonou, Benin
Vandewalle, Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Language :
English
Title :
Biochemical attempt to characterize thirteen cichlid species by their muscular paralbumins
Publication date :
1994
Journal title :
Archives of Physiology and Biochemistry
ISSN :
1381-3455
eISSN :
1744-4160
Publisher :
Swets and Zeitlinger, Netherlands
Volume :
102
Issue :
2
Pages :
135-138
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FRFC - Fonds de la Recherche Fondamentale Collective [BE] FRSM - Fonds de la Recherche Scientifique Médicale [BE] Fondation Alice Seghers
Bailey R.M., Stewart D.J. (1977) Cichlid fishes from lake Tanganyika: additions to the zambian fauna including two new species. Occasional Papers Mus. Zool. Univ. Michigan. Ann Arbor, n° 679:1-30.
Barel C.D.N., Van Oiybn M.P.J., Witte F., Witte-Maas E. (1977) An introduction to the taxonomy and morphology of the haplochromine Cichlidae from Lake Victoria. Neth. J. Zool 27:333-389.
Bhushana Rao K.S.P., Focant B., Gerday C.H., Hamoir G. (1969) Low molecular weight albumins of cod white muscle. (Gadus callarias). Comp. Bioch. Physiol 30:33-48.
Focant B., Joyeux J.C. (1988) Essai de speciation biochimique de six especes de Gobiidés du littoral languedocien. Rapp. Comm. int. Mer Midit 31(2):257.
Focant B., Vandbwalle P. (1989) Comparison of myosin subunits and sarcoplasmic paralbumins of various muscles from two cichlid fishes: Oreochromis niloticus L. and OreochromismossambicusP. Ann. Mus. roy. Afr. Centr. Sc. Zool 257:81-84.
Focant B., Vandewalle P. (1991) Comparison of myosin subunits and sarcoplasmic parvalbumins of white and red trunk muscles from various species of african Cichlids. Application to an hybrid. Ann. Mus. roy. Afr. Centr. Sc. Zool 263:33-38.
Focant B., Voadiu C., Vandewalle P. (1988) Biochemical analysis of parvalbumins and myosin light chains from mediterranean Serranids: first application to the systematic. Rapp. Comm. int. Mer Médit 31(2):258.
Focant B., Michel C.H., Vandewalle P. (1990) Use of the biochemical analysis of muscle proteins to help the classification of polychromic individuals of the genus. Symphodus. Arch. int. Physiol. Biochim 98:87-93.
Gerday C.H. (1982) Soluble calcium-binding proteins from fish and invertebrate muscle. Mol. Physiol 2:63-87.
Greenwood P.H. (1980) Towards a phyletic classification of the “;genus” Haplochromis (Pisces, Cichlidae) and related taxa. Part II. Bull. Br. Mus. nat. Hist. (Zool.) 39:1-99.
Hoyer B.H., McCarthy B.J., Bolton E.T. (1964) A molecular approach in the systematics of higher organisms. Science 144:959-965.
Huriaux F., Focant B. (1977) Isolation and characterization of the three light chains from carp white muscle myosin. Arch. int. Physiol. Biochim 85:917-929.
Kornfield I., Smith D.C., Gagnon P.S. (1982) The cichlid fish of Quatro Ciénegas, Mexico: Direct evidence of conspecifici-ty among distinct trophic morphs. Evolution 36:121-177.
McKays E.R., Kocher T., Reinthal P., Kornfield I. (1982) A sympatric sibling species complex of Petrotilapia Trewawas from Lake Malawi analysed by enzyme electrophoresis (Pisces, Cichlidae). Zool. J. Linn. Soc 76:91-96.
Mednikov B.W., Reshetnikov Y.S., Sawattova K.A. (1977) Molecular DNA hybridization: an approach to disputable issues in fish taxonomy. Cybium 1:111-119.
Poll M. (1956) Poissons Cichlidae. Explorations hydrobiologiques du lac Tanganika (1946–1947). Résultats scientifiques 3(5B):1-619.
Sage R.D., Selander R.K. (1975) Trophic radiation through polymorphism in cichlid fishes. Proc. Natl. Acad. Sci. USA 72:4669-4673.
Thys Van Den Audenaerde D.F.E. Bijdrage tot een systematische en bibliografische monografie van het genus filapia (Pisces, Cichlidae), Ph.D Thesis, Rijksuniversiteit Gent, Belgique; 1970.
Trewawas E. Tilapiine fishes of the genera Sarotherodon, Oreochromis and Danakilia. British Museum of Natural History , Hengry Ling Ltd, The Dorset Press; 1983, 583.
Verheyen E., Vanrompaey J., Aselens M. (1985) Enzyme variation in haplochromine cichlid fishes from Lake Victoria. Neth. J. Zool 35:469-478.
Witte F., Van Ouen M.J.P. (1990) Taxonomy, ecology and fishery of Lake Victoria haplochromine trophic groups. Zool. Verh. Leiden 262:1-47.