The effect of resveratrol on the developmental competence of porcine oocytes vitrified at germinal vesicle stage

[en] We tested the effects of resveratrol both as a pre-treatment and as a recovery treatment after warming during in vitro maturation (IVM) on the viability and developmental competence of porcine oocytes vitrified at the germinal vesicle stage. Pre-treatment before vitrification of oocytes for 3 hr with 2 μM resveratrol did not affect survival, oocyte maturation and embryo developmental competence to the blastocyst stage after parthenogenetic activation. However, supplementation of the medium with resveratrol during subsequent IVM after vitrification and warming significantly improved the ability of surviving oocytes to develop to the blastocyst stage, and this effect was observed only on vitrified, but not on non-vitrified oocytes. The intracellular levels of glutathione and hydrogen peroxide in oocytes were not affected by vitrification and resveratrol treatment. Also, there was no significant difference in the occurrence of apoptosis measured by annexin V binding between vitrified and non-vitrified oocytes, regardless of the resveratrol treatment. In conclusion, resveratrol did not prevent the cellular damages in immature porcine oocytes during vitrification; however, when added to the IVM medium, it specifically improved the developmental competence of vitrified oocytes. Further research will be necessary to clarify the mechanisms of action of resveratrol on the recovery of vitrified oocytes from vitrification-related damages. © 2017 Blackwell Verlag GmbH

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Santos, E. C. S.; Institute of Livestock and Glassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

Appeltant, Ruth ; Université de Liège - ULiège > Dpt. de gestion vétérinaire des Ressources Animales (DRA) > GIGA-R : Génomique animale

Dang-Nguyen, T. Q.; Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

Noguchi, J.; Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

Kaneko, H.; Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

Kikuchi, K.; Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

Somfai, T.; Institute of Livestock and Glassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

Language :

English

Title :

The effect of resveratrol on the developmental competence of porcine oocytes vitrified at germinal vesicle stage

Publication date :

2018

Journal title :

Reproduction in Domestic Animals

ISSN :

0936-6768

eISSN :

1439-0531

Publisher :

Blackwell, Oxford, United Kingdom

Volume :

Issue :

Pages :

304-312

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

JSPS - Japan Society for the Promotion of Science [JA]

Available on ORBi :

since 12 June 2020

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Bibliography

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