Alternative methods to assess the effects of a realistic Persistent Organic Pollutant mixture (POP Mix) following a chronic exposure in zebrafish larvae (Danio rerio).
Guerrero Limon, Gustavo
2020 • SETAC SciCon, SETAC Europe 30th Annual Meeting
zebrafish; fish lateral line; persistent organic pollutant
Abstract :
[en] There is a growing concern related to the so-called Persistent Organic Pollutants (POPs). These compounds originate from different sources, some of them being synthesized with specific purposes and others just randomly appearing as by-products (e.g. incomplete combustion). The research into POPs has shown that they have several adverse effects. Cancer, metabolic disorders, effects in the nervous system have been described amongst many other adverse health effects after POP exposure. The POPs have been generally studied as single/individual compounds, and data is scarce related to mixtures that are not uncommon in the environment and even within humans. Thus, a realistic-environmentally significant Persistent Organic Pollutant mixture (POP Mix) as found in the serum of a Scandinavian population was tested in zebrafish larvae (Danio rerio). The zebrafish has been commonly used in the toxicology field, mainly for toxicity tests where the endpoint is lethality. In this study, a set of different approaches was used to assess the effects of a POP mix exposure on zebrafish larvae after 96 hours post-fertilization. In our scope, we mainly focused in endpoints such as development of the lateral line system, malformations, etc. Our results suggest that there are no major effects on the development of the lateral line at any of the different concentrations tested. However, some other morphological traits were undergoing deficiencies during development, such as edemas (pericardial or yolk sac). Thus, we have proven that the POP mix has some deleterious effects at realistic concentrations and further analysis is required.
Research Center/Unit :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Environmental sciences & ecology
Author, co-author :
Guerrero Limon, Gustavo ; Université de Liège - ULiège > I3-Laboratory for Organogenesis and Regeneration
Language :
English
Title :
Alternative methods to assess the effects of a realistic Persistent Organic Pollutant mixture (POP Mix) following a chronic exposure in zebrafish larvae (Danio rerio).
Publication date :
05 May 2020
Event name :
SETAC SciCon, SETAC Europe 30th Annual Meeting
Event organizer :
Society of Environmental Toxicology and Chemistry
Event place :
Dublin, Ireland
Event date :
from 03-05-2020 to 07-05-2020
Audience :
International
European Projects :
H2020 - 722634 - PROTECTED - PROTECTion against Endocrine Disruptors; Detection, mixtures, health effects, risk assessment and communication.
Funders :
CE - Commission Européenne [BE]
Funding text :
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 722634.