Chitosan-collagen 3d matrix mimics trabecular bone and regulates rankl-mediated paracrine cues of differentiated osteoblast and mesenchymal stem cells for bone marrow macrophage-derived osteoclastogenesis

Elango, J.; Saravanakumar, K.; Rahman, S. U.; Henrotin, Yves; Regenstein, J. M.; Wu, W.; Bao, B.

doi:10.3390/biom9050173

Article (Scientific journals)

Chitosan-collagen 3d matrix mimics trabecular bone and regulates rankl-mediated paracrine cues of differentiated osteoblast and mesenchymal stem cells for bone marrow macrophage-derived osteoclastogenesis

Elango, J.; Saravanakumar, K.; Rahman, S. U. et al.

2019 • In Biomolecules, 9 (5)

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/246782

DOI
10.3390/biom9050173

PubMed
31060346

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Keywords :

Biomechanical properties; Bone homeostasis; Chitosan-composite 3D matrix; Mesenchymal stem cells; Osteoclast; Ovariectomized mice; RANKL; Rheological properties; Runx2

Abstract :

[en] Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption. Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia. In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals. In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages. The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix. The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively. Besides, osteogenic progenitor’s paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix. From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Disciplines :

Rheumatology

Author, co-author :

Elango, J.; Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China, Bone Biology and Disease Unit, St. Vincent’s Institute of Medical Research, Melbourne, VIC 3065, Australia

Saravanakumar, K.; Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon 24341, South Korea

Rahman, S. U.; Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Islamabad, 45550, Pakistan

Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartilage (U.R.O.C.)

Regenstein, J. M.; Department of Food Science, Cornell University, Ithaca, NY 14853-7201, United States

Wu, W.; Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

Bao, B.; Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

Language :

English

Title :

Publication date :

2019

Journal title :

Biomolecules

eISSN :

2218-273X

Publisher :

MDPI AG

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

NSCF - National Natural Science Foundation of China [CN]

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