Reference : Exploring with [(18)F]UCB-H the in vivo Variations in SV2A Expression through the Kai...
Scientific journals : Article
Human health sciences : Radiology, nuclear medicine & imaging
http://hdl.handle.net/2268/246545
Exploring with [(18)F]UCB-H the in vivo Variations in SV2A Expression through the Kainic Acid Rat Model of Temporal Lobe Epilepsy.
English
Serrano Navacerrada, Maria Elisa [Université de Liège - ULiège > > CRC In vivo Imaging-Preclinical Imaging >]
Bahri, Mohamed Ali mailto [Université de Liège - ULiège > > CRC In vivo Imaging-Aging & Memory >]
Becker, Guillaume [Université de Liège - ULiège > Département de Psychologie > Département de Psychologie >]
Seret, Alain mailto [Université de Liège - ULiège > Département de physique > Imagerie médicale expérimentale >]
Germonpre, Charlotte [> >]
Lemaire, Christian mailto [Université de Liège - ULiège > > CRC In vivo Imaging-Radiochemistry >]
Giacomelli, Fabrice mailto [> >]
Mievis, Frédéric mailto [> >]
Luxen, André [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse >]
Salmon, Eric mailto [Université de Liège - ULiège > Département des sciences cliniques > Neuroimagerie des troubles de la mémoire et revalid. cogn. >]
Rogister, Bernard mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Raedt, Robrecht [> >]
Plenevaux, Alain mailto [Université de Liège - ULiège > > CRC In vivo Imaging-Preclinical Imaging >]
23-Mar-2020
Molecular imaging and biology
Yes (verified by ORBi)
International
1536-1632
1860-2002
United States
[en] EEG ; Kainic acid ; SV2A ; SV2A immunofluorescence ; T2 MRI ; Temporal lobe epilepsy ; [18F]UCB-H
[en] PURPOSE: The main purpose of this study was to understand how the positron emission tomography (PET) measure of the synaptic vesicle 2A (SV2A) protein varies in vivo during the development of temporal lobe epilepsy (TLE) in the kainic acid rat model. PROCEDURES: Twenty Sprague Dawley male rats were administered with multiple systemic doses of saline (control group, n = 5) or kainic acid (5 mg/kg/injection, epileptic group, n = 15). Both groups were scanned at the four phases of TLE (early, latent, transition, and chronic phase) with the [(18)F]UCB-H PET radiotracer and T2-structural magnetic resonance imaging. At the end of the scans (3 months post-status epilepticus), rats were monitored for 7 days with electroencephalography for the detection of spontaneous electrographic seizures. Finally, the immunofluorescence staining for SV2A expression was performed. RESULTS: Control rats presented a significant increase in [(18)F]UCB-H binding at the last two scans, compared with the first ones (p < 0.001). This increase existed but was lower in epileptic animals, producing significant group differences in all the phases of the disease (p < 0.028). Furthermore, the quantification of the SV2A expression in vivo with the [(18)F]UCB-H radiotracer or ex vivo with immunofluorescence led to equivalent results, with a positive correlation between both. CONCLUSIONS: Even if further studies in humans are required, the ability to detect a progressive decrease in SV2A expression during the development of temporal lobe epilepsy supports the use of [(18)F]UCB-H as a useful tool to differentiate, in vivo, between healthy and epileptic animals along with the development of the epileptic disease.
Centre de Recherches du Cyclotron - CRC
F.R.S.-FNRS - Fonds de la Recherche Scientifique ; ULiège - Université de Liège
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/246545
10.1007/s11307-020-01488-7

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Serrano2020_Article_ExploringWith[18F]UCB-HTheInVi.pdfPublisher postprint1.24 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.