Denitrification; France coast; Gironde Estuary; Maximum turbidity zonel; Nitrificationc; Nitrous oxide; Resuspension; Sedimentation; Tidal cycle
Abstract :
[en] The maximum turbidity zone (MTZ) of the Gironde Estuary is a site of important mineralization of particulate organic nitrogen. Moreover, this MTZ is characterized by intense cycles of settling and resuspension of anoxic fluid mud at both tidal and neap-spring time-scales. In the upper layer of the fluid mud, which was deposited at tidal slack and eroded during each ebb and flood, a denitrification rate of up to 65 μmol 1-1 h-1 was measured during the transient period of settling. High concentrations of NO2- (up to 13 μM), and N2O (up to 1500 nM) were observed in this layer at tidal slack, either due to an incomplete nitrification at low oxygen concentrations or to high denitrification activity. This suggests that deposition and erosion of the upper fluid mud at the tidal time-scale could be an important source of N2O for the estuarine overlying waters. The deepest layer of the fluid mud was more turbid, NO3- depleted and remained stable throughout tidal cycles, during several days. In this layer, ammonification was responsible for an increase of NH4/+. Most probable number (MPN) counts of ammonium oxidizing bacteria in turbid water and fluid mud revealed that they were closely associated with particles, whereas potential nitrification rates were rather constant (10-14 μmol 1-1 h-1) irrespective of the suspended matter concentrations. In the MTZ of the Gironde, nitrogen behaves like in activated sludge reactors, with oxic/anoxic oscillations occurring at the tidal time scale. Nitrification takes place in the oxic water column and denitrification in the anoxic fluid mud. However at the whole estuarine scale the loss of particulate nitrogen and the gain of total dissolved inorganic nitrogen are in the same order of magnitude, which suggests that the net loss of nitrogen from the system is rather limited. This seems due to the fact that high denitrification rates are limited both in time (tidal slacks) and space (upper layers of fluid mud). However, our study emphasises the importance of settling/erosion transition periods on the nitrogen dynamics in estuarine maximum turbidity zones.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Abril, Gwénaël; Université Bordeaux 1
Riou, S; Université Bordeaux 1
. Etcheber, Henri; Université Bordeaux 1
Frankignoulle, Michel; Université de Liège - ULiège
De Wit, Rutger; Université Bordeaux 1
Middelburg, Jack J.; Netherlands Institute of Ecology
Language :
English
Title :
Transient, tidal time-scale Nitrogen transformations in an estuarine turbidity maximum-fluid mud system (The Gironde, France)
Abril G., Etcheber H., Le Hir P., Bassoullet P., Boutier B., Frankignoulle M. (1999) Oxic/anoxic oscillations and organic carbon mineralization in an estuarine maximum turbidity zone (The Gironde, France). Limnology and Oceanography 44:1304-1315.
Allen G.P., Etude des processus sedimentaires dans l'estuarie de la Gironde. Memoire IGBA, University of Bordeaux I; 1972, 314.
Allen G.P., Salomon J.C., Bassoulet P., Du Penhoat Y., De Grandpre C. (1980) Effects of tides on mixing and suspended sediment transport in macrotidal estuaries. Sedimentary Geology 26:69-90.
Balls P.W. (1992) Nutrient behaviour in two contrasting Scottish estuaries, the Forth and Tay. Oceanologica Acta 15:261-277.
Balls P.W. (1994) Nutrient inputs to estuaries from nine Scottish east coast rivers; influence of estuarine processes on inputs to the North Sea. Estuarine, Coastal and Shelf Science 39:329-352.
Balls P.W., Brockie N., Dobson J., Johnston W. (1996) Dissolved oxygen and nitrification in the upper Forth Estuary during summer (1982-92): Patterns and trends. Estuarine, Coastal and Shelf Science 42:117-134.
Bange H.W., Rapsomanikis S., Andreae M.O. (1996) Nitrous oxide in coastal waters. Global Biogeochemical Cycles 10:197-207.
Billen G. (1975) Nitrification in the Scheldt Estuary (Belgium and the Netherlands). Estuarine, Coastal and Shelf Science 3:79-89.
Billen G., Somville M., De Becker E., Servais P. (1985) A nitrogen budget of the Scheldt hydrographical basin. Netherlands Journal of Sea Research 19:223-230.
Cheng S.S., Lin Y.T., Chen S.K. (1996) Enhanced biodegradation of organic nitrogenous compounds in resin manufacturing wastewater by anoxic denitrification and oxic nitrification processes. Water Science and Technology 34:35-41.
Clarke K.R., Owens N.J.P. (1983) A simple and versatile micro-computer program for the determination of 'Most Probable Number'. Journal of Microbiological Methods 1:133-137.
Cole J.J., Caraco N.F., Peierls B.L. (1992) Can phytoplankton maintain a positive carbon balance in a turbid, freshwater, tidal estuary?. Limnology and Oceanography 37:1608-1617.
Etcheber H., Biogeochimie de la matiere organique en milieu estuarien: comportement, bilan, proprietes. Ph.D. Thesis. University of Bordeaux I; 1983, 352.
Fishez R., Jickells T.D., Edmunds H.M. (1992) Algal blooms in high turbidity, a result of the conflicting consequences of turbulence on nutrient cycling in a shallow water estuary. Estuarine, Coastal and Shelf Science 35:577-592.
Frankignoulle M., Abril G., Borges A., Bourge I., Canon C., Delille B., Libert E., Theate J.M. (1998) Carbon dioxide emission from European estuaries. Science 282:434-436.
Gattuso J.-P., Frankignoulle M., Wollast R. (1998) Carbon and carbonate metabolism in coastal aquatic ecosystems. Annual Review of Ecology and Systematics 29:405-434.
Grabemann I., Uncles R.J., Krauss G., Stephens J.A. (1997) Behaviour of turbidity maxima in the Tamar (U.K.) and Weser (F.R.G.) estuaries. Estuarine, Coastal and Shelf Science 45:235-246.
Hansen J.I., Henriksen K., Blackburn T.H. (1981) Seasonal distribution of nitrifying bacteria and rates of nitrification in coastal marine sediments. Microbial Ecology 7:297-304.
Helder W., De Vries R.T.P. (1983) Estuarine nitrite maxima and nitrifying bacteria (Ems-Dollard estuary). Netherland Journal of Sea Research 17:1-18.
Henriksen K. (1980) Measurement of in situ rates of nitrification in sediment. Microbial Ecology 6:329-337.
1994, 115.
Irigoien X., Castel J. (1997) Light limitation and distribution of chlorophyll pigments in a highly turbid estuary: The Gironde (SW France). Estuarine, Coastal and Shelf Science 44:507-517.
Jouanneau J.M., Latouche C. (1981) The Gironde Estuary., Contribution to Sedimentology (Furchtbauer, H., Lisitzyn, A. P., Millerman, J. D. and Seibold, E., eds), Stuttgart; 10:1-115.
King D., Nedwell D.B. (1985) The influence of nitrate concentration upon the end-products of nitrate dissimilation by bacteria in a anaerobic salt marsh sediment. FEMS Microbiology Letters 31:23-28.
Koike I., Terauchi K. (1996) Fine scale distribution of nitrous oxide in marine sediments. Marine Chemistry 52:185-193.
Koike I., Sorensen J. (1988) Nitrate reduction and denitrificafion in marine sediments., Nitrogen Cycling in Coastal Marine Environments (Blackburn, T. H. and Sorensen, J., eds). SCOPE, Wiley; 3:251-273.
Koops H.P., Moller U.C. (1992) The lithotrophic ammoniaoxidising bacteria., The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications (Balows, A., Truper, H. G., Dworkin, M., Harder, W. and Schleifer, K. H., eds), 2nd edition. Springer Verlag, New York; 2625-2637.
Law C.S., Rees A.P., Owens N.P.J. (1992) Nitrous oxide: Estuarine sources and atmospheric flux. Estuarine, Coastal and Shelf Science 35:301-314.
Lin R.G., Etude du potentiel de degradation de la matiere organique particulaire au passage eau douce-eau salee: cas de l'estuaire de la Gironde. Ph.D. Thesis, University of Bordeaux I; 1988, 209.
MacFarlane G.T., Herbert R.A. (1984) Comparative study of enrichment methods for the isolation of autotrophic nitrifying bacteria from soil, estuarine and marine sediments. FEMS Microbiology Letters 22:127-132.
Maneux E., Erosion mecanique des sols et transports fluviaux de matieres en suspension: application des systemes d'information geographique dans les bassins versant de l'Adour, de la Dordogne et de la Garonne. Ph.D. Thesis, University of Bordeaux I; 1998, 252.
Maurice L. (1994) Biodegradabilite de la matiere organique dans le bouchon vaseux et la creme de vase de l'estuaire de la Loire. Oceanologica Acta 17:501-516.
Meybeck M., Cauwet G., Dessery S., Somville M., Gouleau D., Billen G. (1988) Nutrients (Organic C, P, N, Si) in the eutrophic river Loire and its estuary. Estuarine, Coastal and Shelf Science 27:595-624.
Middelburg J.J., Nieuwenhuize J. (2000) Uptake of dissolved inorganic nitrogen in turbid, tidal estuaries. Marine Ecology Progress Series 132:79-88.
Middelburg J.J., Klaver G., Nieuwenhuize J., Markusse R.M., Vlug T., Van der Nat F.J.W.A. (1995) Nitrous oxide emissions from estuarine intertidal sediments. Hydrobiologia 311:43-55.
Middelburg J.J., Soetaert K., Herman P.M.J., Heip C.H.R. (1996) Marine sedimentary denitrification: A model study. Global Biogeochemical Cycles 10:661-673.
Middelburg J.J., Soetaert K., Herman P.M.J. (1997) Empirical relationships for use in global diagenetic models. Deep-Sea Research 44:327-344.
Morris A.W., Loring D.H., Bales A.J., Howland R.J.M., Mantoura R.F.C., Woodward E.M.S. (1982) Particle dynamic, particulate carbon and the oxygen minimum in an estuary. Oceanologica Acta 5:349-353.
Nedwell D.B., Trimmer M. (1996) Nitrogen fluxes through the upper estuary of the Great Ouse, England: The role of bottom sediments. Marine Ecology Progress Series 142:273-286.
Nielsen L.P., Glud R.N. (1996) Denitrification in a coastal sediment measured in situ by the nitrogen isotope pairing technique applied to a benthic flux chamber. Marne Ecology Progress Series 137:181-186.
Nixon S.W., Ammerman J.W., Atkinson L.P., Berounsky V.M., Billen G., Boicourt W.C., Boynton W.R., Church T.M., DiToro D.M., Elmgren R., Garber J.H., Giblin A.E., Jahnke R.A., Owens N.J.P., Pilson M.E.Q., Seitzinger S.P. (1996) The fate of nitrogen and phosphorus at the landsea margin of the North Atlantic Ocean. Biogeochemistry 35:141-180.
Nowicki B.L., Oviatt C.A. (1990) Are estuaries traps for anthropogenic nutrients? Evidence from estuarine mesocosms. Marine Ecology Progress Series 66:131-146.
Ogilvie B., Nedwell D.B., Harrison R.M., Robinson A., Sage A. (1997) High nitrate, muddy estuaries as nitrogen sinks: The nitrogen budget of the River Colne estuary (United Kingdom). Marine Ecology Progress Series 150:217-228.
Owens N.J.P. (1986) Estuarine nitrification: A naturally occurring fiuidized bed reaction?. Estuarine, Coastal and Shelf Science 22:31-44.
Parker W.R., Marshall L.D., Parfitt A.J. (1994) Modulation of dissolved oxygen levels in a hypertidal estuary by sediment resuspension. Netherland Journal of Aquatic Ecology 38:347-352.
Philipps, Qualite des eaux dans l'estuaire de la Gironde. Repartition et comportement des sels mineraux dissous: azote, phosphore et silice. Ph.D. Thesis, University of Bordeaux I; 1980, 189.
Robinson A.D., Nedwell D.B., Harrison R.M., Ogilvie B.G. (1998) Hypernutrified estuaries as sources of N2O to the atmosphere: The estuary of the River Colne, Essex, U.K. Marine Ecology Progress Series 164:59-71.
Rowe R., Todd R., Waide J. (1977) Microtechnique for most probable number analysis. Applied and Environmental Microbiology 33:675-680.
Seitzinger S.P. (1988) Denitrification in freshwater and marine ecosystems: Ecological and geochemical significance. Limnology and Oceanography 33:702-724.
Smith C.J., De Laune R.D., Patrick W.H. Jr. (1982) Nitrate reduction in Spartina alterniflora march soil. Soil Science Society of America Journal 46:748-750.
Smith C.J., De Laune R.D., Patrick W.H. Jr. (1985) Fate of riverine nitrate entering an estuary: I. Denitrification and nitrogen burial. Estuaries 8:15-21.
Soetaert K., Herman P.M.J. (1995) Nitrogen dynamics in the Westerschelde estuary (SW Netherlands) estimated by means of the ecosystem model MOSES. Hydrobiologia 311:225-246.
Stehr G., Bottcher B., Dittberner P., Rath G., Koops H.P. (1995) The ammonia-oxidising nitrifying population of the river Elbe estuary. FEMS Microbiology Ecology 17:177-186.
Strickland J.D.H., Parsons T.R. (1972) A practical handbook of sea water analysis. Bulletin of Fishery Research of Canada 167:310.
Sylvester A.J., Ware G.C. (1976) Anaerobiosis of fluid mud. Nature 264:635.
Thouvenin B., LeHir P., Romana L.A. (1994) Dissolved oxygen model in the Loire Estuary., Changes in fluxes in estuaries: Implications from science to managements (Dyer, K. R. and Orth, R. J., eds), Academic Press, New York; 169-178.
Veyssy E., Transferts de matieres organiques des bassins versants aux estuaires de la Gironde et de l'Adour. Ph.D. Thesis, University of Bordeaux I; 1998, 262.
Wollast R. (1983) Interaction in estuaries and coastal waters., The Major Biogeochemical Cycles and their Interactions (Bolin, B. and Cook, R. B., eds). SCOPE, Wiley; 385-407.
Weiss R.F., Price B.A. (1980) Nitrous oxide solubility in water and seawater. Marine Chemistry 8:347-359.
