Article (Scientific journals)
Pseudomonas chlororaphis as a multiproduct platform: Conversion of glycerol into high-value biopolymers and phenazines
de Meneses, L.; Pereira, J. R.; Sevrin, Chantal et al.
2020In New Biotechnology, 55, p. 84-90
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Keywords :
Co-production; Extracellular polysaccharide; Medium-chain-length polyhydroxyalkanoate; Phenazines; Pseudomonas chlororaphis; Biopolymers; Cell proliferation; Chain length; Crystallinity; Glycerol; Extracellular polysaccharides; Poly-hydroxyalkanoate; Bacteria; Article
Abstract :
[en] Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated using glycerol as the sole carbon source for the simultaneous production of medium-chain length polyhydroxyalkanoates (mcl-PHA), extracellular polysaccharide (EPS) and phenazines. A maximum cell dry mass of 11.79 g/L was achieved with a mcl-PHA content of 19 wt%, corresponding to a polymer concentration of 2.23 g/L. A considerably higher EPS production, 6.10 g/L, was attained. Phenazines synthesis was evidenced by the bright orange coloration developed by the culture during the cell growth phase. The mcl-PHA produced by P. chlororaphis was composed mainly of 3-hydroxydecanoate (50 wt%) with lower amounts of 3-hydroxyoctanoate (17 wt%), 3-hydroxytetradecanoate (17 wt%), 3-hydroxydodecanoate (13 wt%) and 3-hydroxyhexanoate (3 wt%). This PHA showed unique thermal features being highly amorphous, with a degree of crystallinity of 27% and a low melting temperature (45.0 °C). The secreted EPS was mostly composed of glucose, glucosamine, rhamnose and mannose, with smaller amounts of three other unidentified monomers. Although the bioprocess can be improved further to define the optimal conditions to produce each bioproduct (mcl-PHA, EPS or phenazines), this study has demonstrated for the first time the ability of P. chlororaphis to simultaneously produce three high-value products from a single substrate. © 2019 Elsevier B.V.
Research Center/Unit :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Materials science & engineering
Author, co-author :
de Meneses, L.;  Applied Molecular Biosciences Unit (UCIBIO-REQUIMTE), NOVA University of Lisbon, Campus da Caparica, Caparica, Portugal, Associate Laboratory for Green Chemistry (LAQV-REQUIMTE), Chemistry Department, Faculty of Sciences and Technology, NOVA University of Lisbon, Campus da Caparica, Caparica, Portugal
Pereira, J. R.;  Applied Molecular Biosciences Unit (UCIBIO-REQUIMTE), NOVA University of Lisbon, Campus da Caparica, Caparica, Portugal
Sevrin, Chantal ;  Université de Liège - ULiège > CEIB
Grandfils, Christian ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Paiva, A.;  Associate Laboratory for Green Chemistry (LAQV-REQUIMTE), Chemistry Department, Faculty of Sciences and Technology, NOVA University of Lisbon, Campus da Caparica, Caparica, Portugal
Reis, M. A. M.;  Applied Molecular Biosciences Unit (UCIBIO-REQUIMTE), NOVA University of Lisbon, Campus da Caparica, Caparica, Portugal
Freitas, F.;  Applied Molecular Biosciences Unit (UCIBIO-REQUIMTE), NOVA University of Lisbon, Campus da Caparica, Caparica, Portugal
Language :
English
Title :
Pseudomonas chlororaphis as a multiproduct platform: Conversion of glycerol into high-value biopolymers and phenazines
Publication date :
2020
Journal title :
New Biotechnology
ISSN :
1871-6784
eISSN :
1876-4347
Publisher :
Elsevier B.V.
Volume :
55
Pages :
84-90
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 March 2020

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