Bionanoparticles; HPV; Heterologous expression systems; Nepovirus; Protein refolding; Vaccine antigen
Abstract :
[en] BACKGROUND: Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new carrier for the presentation of human papillomavirus (HPV) L2 epitope was studied. To achieve this goal, a model of the HPV L2 epitope secondary structure was predicted and its insertion within 5 external loops in the GFLV capsid protein (CP) was evaluated. RESULTS: The epitope sequence was genetically inserted in the alphaB-alphaB(") domain C of the GFLV CP, which was then over-expressed in Pichia pastoris and Escherichia coli. The highest expression yield was obtained in E. coli. Using this system, VLP formation requires a denaturation-refolding step, whereas VLPs with lower production yield were directly formed using P. pastoris, as confirmed by electron microscopy and immunostaining electron microscopy. Since the GFLV L2 VLPs were found to interact with the HPV L2 antibody under native conditions in capillary electrophoresis and in ELISA, it can be assumed that the inserted epitope is located at the VLP surface with its proper ternary structure. CONCLUSIONS: The results demonstrate that GFLV VLPs constitute a potential scaffold for surface display of the epitope of interest.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Yazdani, Razieh
Shams-Bakhsh, Masoud
Hassani-Mehraban, Afshin
Arab, Seyed Shahriar
Thelen, Nicolas ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Jacobs, Nathalie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Virologie
Brans, Alain ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein.
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