Article (Scientific journals)
Comparison of isotope pattern deconvolution and calibration curve quantification methods for the determination of estrone and 17β-estradiol in human serum
Pitarch-Motellón, Jorge; Fabregat Cabello, Neus; Le Goff, Caroline et al.
2019In Journal of Pharmaceutical and Biomedical Analysis, 171, p. 164-170
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Keywords :
Estrone; Isotope dilution mass spectrometry; Isotope pattern deconvolution; LC–MS/MS; Serum
Abstract :
[en] A Liquid Chromatography coupled to tandem mass spectrometry (LC–MS/MS) based method have been developed for the determination of the main estrogen compounds –estrone (E1) and 17β-estradiol (E2)– in human serum. Two isotope dilution mass spectrometry (IDMS) quantification procedures have been used: a classical calibration curve-based method were compared to a recently developed isotope pattern deconvolution (IPD) method. IPD is based on isotopic abundance measurements and multiple linear regression. Validation was performed in terms of intra-assay repeatability (n = 5), inter-assay reproducibility (n = 9) and accuracy using spiked steroid-free serum at 5 concentration levels and 3 certified reference materials. Both methodologies meet EMEA requirements yielding recoveries between 79–106% and coefficient of variations of 1.7–8.3% along all experiments. Limits of quantification as low as 5 ng/L were achieved. 40 real samples were analysed for comparison purposes showing a great correlation between calibration and IPD concentration values. Real samples were also quantified by routine immunoassay analysis, which showed a significant proportional bias of 2.55 for E1 and good correlation for E2. While methods were considered suitable for routine or countercheck analysis within the context of hospital's needs, IPD has demonstrated to be faster and cost saving. © 2019
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Pitarch-Motellón, Jorge;  Research Institute for Pesticides and Water, Universitat Jaume I, Castelló, Spain
Fabregat Cabello, Neus ;  Department of Clinical Chemistry, University of Liège, CHU Sart-Tilman, Liège, Belgium, Hematology and Hemotherapy group, Health Research Institute Hospital La Fe (IIS La Fe), Valencia, Spain
Le Goff, Caroline  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Roig-Navarro, A. F.;  Research Institute for Pesticides and Water, Universitat Jaume I, Castelló, Spain
Sancho-Llopis, J. V.;  Research Institute for Pesticides and Water, Universitat Jaume I, Castelló, Spain
Cavalier, Etienne  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Language :
English
Title :
Comparison of isotope pattern deconvolution and calibration curve quantification methods for the determination of estrone and 17β-estradiol in human serum
Publication date :
2019
Journal title :
Journal of Pharmaceutical and Biomedical Analysis
ISSN :
0731-7085
eISSN :
1873-264X
Publisher :
Elsevier B.V.
Volume :
171
Pages :
164-170
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 February 2020

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