Re-certification of hydroxyvitamin D standards by isotope pattern deconvolution

Certification; Isotope dilution mass spectrometry; Isotope Pattern Deconvolution; LC-MS/MS; Quality control; Vitamin D; Mass spectrometry; Standardization; Vitamins; Isotope pattern; Vitamin-D; Isotopes; Article

Abstract :

[en] Background: Vitamin D testing in analytical clinical laboratories has been experiencing a rapid increase of demand over the last years, as it plays a key role in several disorders. Due to the narrow ranges of medical significance regarding its concentration levels in human serum, accurate and precise determinations of vitamin D metabolites are required. Methods: We present an isotope dilution mass spectrometry quantification method for the re-certification of routine commercial standards used in method validation steps, isotope pattern deconvolution (IPD) based on LC-MS/MS. Results: IPD allowed to compensate for the observed biases of +4.7% for 25(OH)D3, −29% for 25(OH)D2 and −30% for 24,25(OH) 2 D 3 standard concentrations, respectively in an easy, cheap and straightforward way. Conclusions: Is has been observed that, in some cases, discrepancies may exist between stated purity or amount of routinely used commercial standards and actual values, which would lead to unwanted bias in the developed methodologies. The present correction has helped meeting the regulations established by international standardization programs, including Vitamin D Standardization Program (VDSP). © 2019 Elsevier B.V.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

Pitarch-Motellón, Jorge; Research Institute for Pesticides and Water, Universitat Jaume I, Castelló, Spain

Roig-Navarro, Antoni Francesc; Research Institute for Pesticides and Water, Universitat Jaume I, Castelló, Spain

Le Goff, Caroline ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

CAVALIER, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de chimie clinique

Fabregat Cabello, Neus ; Department of Clinical Chemistry, University of Liège, CHU Sart-Tilman, Liège, Belgium

Language :

English

Title :

Re-certification of hydroxyvitamin D standards by isotope pattern deconvolution

Publication date :

2019

Journal title :

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN :

1570-0232

eISSN :

1873-376X

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

1120

Pages :

89-94

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 February 2020

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