Article (Scientific journals)
RNA-Seq-Based Comparative Transcriptome Analysis Highlights New Features of the Heat-Stress Response in the Extremophilic Bacterium Deinococcus radiodurans.
Xue, Dong; Liu, Wenzheng; Chen, Yun et al.
2019In International Journal of Molecular Sciences, 20 (22)
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Keywords :
Deinococcus radiodurans; RNA-Seq; cell wall damage; heat stress; novel heat-related gene; recovery
Abstract :
[en] Deinococcus radiodurans is best known for its extraordinary resistance to diverse environmental stress factors, such as ionizing radiation, ultraviolet (UV) irradiation, desiccation, oxidation, and high temperatures. The heat response of this bacterium is considered to be due to a classical, stress-induced regulatory system that is characterized by extensive transcriptional reprogramming. In this study, we investigated the key functional genes involved in heat stress that were expressed and accumulated in cells (R48) following heat treatment at 48 degrees C for 2 h. Considering that protein degradation is a time-consuming bioprocess, we predicted that to maintain cellular homeostasis, the expression of the key functional proteins would be significantly decreased in cells (RH) that had partly recovered from heat stress relative to their expression in cells (R30) grown under control conditions. Comparative transcriptomics identified 15 genes that were significantly downregulated in RH relative to R30, seven of which had previously been characterized to be heat shock proteins. Among these genes, three hypothetical genes (dr_0127, dr_1083, and dr_1325) are highly likely to be involved in response to heat stress. Survival analysis of mutant strains lacking DR_0127 (a DNA-binding protein), DR_1325 (an endopeptidase-like protein), and DR_1083 (a hypothetical protein) showed a reduction in heat tolerance compared to the wild-type strain. These results suggest that DR_0127, DR_1083, and DR_1325 might play roles in the heat stress response. Overall, the results of this study provide deeper insights into the transcriptional regulation of the heat response in D. radiodurans.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Xue, Dong ;  Université de Liège - ULiège > Terra
Liu, Wenzheng
Chen, Yun
Liu, Yingying
Han, Jiahui
Geng, Xiuxiu
Li, Jiang
Jiang, Shijie
Zhou, Zhengfu
Zhang, Wei
Chen, Ming
Lin, Min
Ongena, Marc ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Wang, Jin
More authors (4 more) Less
Language :
English
Title :
RNA-Seq-Based Comparative Transcriptome Analysis Highlights New Features of the Heat-Stress Response in the Extremophilic Bacterium Deinococcus radiodurans.
Publication date :
2019
Journal title :
International Journal of Molecular Sciences
ISSN :
1661-6596
eISSN :
1422-0067
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Volume :
20
Issue :
22
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 February 2020

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