Article (Scientific journals)
Syringopeptin contributes to the virulence of Pseudomonas fuscovaginae, based on sypA biosynthesis mutant analysis.
Weeraratne, Nirodha; Stodart, Ben John; Venturi, Vittorio et al.
2020In Phytopathology
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Keywords :
Analytical and theoretical plant pathology; Bacteriology; Genetics and resistance
Abstract :
[en] Pseudomonas fuscovaginae, first reported from Japan in 1976, is now present in many agro-ecological regions around the world; it causes sheath brown rot of rice and is reported as a pathogen of a broad range of hosts. The pathogen can infect rice plants at all stages of growth and is known to cause significant losses due to grain discoloration, poor spike emergence and panicle sterility. Limited information is available on the virulence and mechanisms of pathogenicity for P. fuscovaginae. To address this, an analysis of available genomes was conducted which identified the presence of a gene, showing homology to one of the genes contributing to syringopeptin synthetase (sypA) of P. syringae pv. syringae. To study the potential role of this gene in the virulence and pathogenicity of P. fuscovaginae, a site-specific mutation was created. Following inoculation of seeds and plantlets of rice and wheat with P. fuscovaginae wild types and their respective mutants, we demonstrated that the mutation significantly reduced virulence. This was evident on rice and wheat inoculated with mutants causing a significantly higher number of roots, length of roots and seedling height compared to their respective wild types. Characteristic disease symptoms of necrotic lesions were significantly less in rice seedlings infected with bacterial suspensions of mutants indicating a reduction in virulence. Chromatography analysis of bacterial exudates showed suppression of synthesis of metabolites analogous to syringopeptin in the mutants. These data demonstrate that the protein encoded by this sypA homologue gene is a major virulence determinant of P. fuscovaginae.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Weeraratne, Nirodha
Stodart, Ben John
Venturi, Vittorio
Hofte, Monica
Hua, Gia Khuong Hoang
Ongena, Marc ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Savocchia, Sandra
Steel, Christopher
Ash, Gavin J.
Language :
English
Title :
Syringopeptin contributes to the virulence of Pseudomonas fuscovaginae, based on sypA biosynthesis mutant analysis.
Publication date :
2020
Journal title :
Phytopathology
ISSN :
0031-949X
eISSN :
1943-7684
Publisher :
American Phytopathological Society, United States
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 February 2020

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