Article (Scientific journals)
A Novel Noncoding RNA dsr11 Involved in Heat Stress Tolerance in Deinococcus radiodurans.
Xue, Dong; Chen, Yun; Li, Jiang et al.
2019In Biomolecules, 10 (1)
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Keywords :
Deinococcus radiodurans; dr_0651; dsr11; heat stress; noncoding RNA; trmE
Abstract :
[en] Deinococcus radiodurans is an extremely resistant bacteria that has evolved masterful strategies to enable survival under various environmental stress conditions. Heat stress is a major environmental stress factor that can cause denaturation of proteins, membrane disruption, and oxidative stress. Previous studies have examined the mechanisms of the heat stress response by analyzing changes in protein levels; however, little is known about the role of small noncoding RNAs (ncRNAs), which are known to play important regulatory functions in bacteria during various environmental stress response. The ncRNA dsr11 of D. radiodurans was previously identified by RNA-seq and Northern blot. In this study, we showed that the transcription level of dsr11 was up-regulated 4.2-fold under heat stress by qRT-PCR analysis. Heat tolerance assay showed that deleting dsr11 significantly inhibited the viability under high temperature conditions. To assess the influence of dsr11 on the D. radiodurans transcriptome, 157 genes were found differentially expressed in the knock-out mutant by RNA-seq experiment. Combining RNA-seq and in silico analysis, we found that trmE (tRNA modification GTPase) and dr_0651 (arginase) were likely to be the direct targets of dsr11. Further microscale thermophoresis results demonstrated that dsr11 can directly bind to the mRNA of trmE and dr_0651. Our results indicated that dsr11 can enhance the tolerance to heat stress of D. radiodurans by binding to trmE and dr_0651 mRNA. Overall, these results extend our understanding of ncRNA regulation and provide new insights into the heat stress response in D. radiodurans.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Xue, Dong ;  Université de Liège - ULiège > Terra
Chen, Yun
Li, Jiang
Han, Jiahui
Zhou, Zhengfu
Zhang, Wei
Chen, Ming
Lin, Min
Ongena, Marc ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Wang, Jin
Language :
English
Title :
A Novel Noncoding RNA dsr11 Involved in Heat Stress Tolerance in Deinococcus radiodurans.
Publication date :
2019
Journal title :
Biomolecules
eISSN :
2218-273X
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Volume :
10
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 February 2020

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