Paper published in a book (Scientific congresses and symposiums)
Group B streptococcus neonatal invasive infections in Belgium 2010-2017, and characterization of isolated strains.
Melin, Pierrette; SACHELI, Rosalie; Lambotte, Olivia et al.
2019In INMIS, International Committee (Ed.) INMIS 2019 Abstract book
Peer reviewed
 

Files


Full Text
INMIS final 2019 P.Melin.pdf
Author postprint (308.79 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
group B Streptococcus; neonatal infections; Belgium
Abstract :
[en] Introduction/Background & Aims: Where intrapartum antibiotic-prophylaxis (IAP) is given to pregnant women colonized with Group B Streptococcus (GBS), the incidence of neonatal early-onset disease (EOD) has been successfully reduced; nevertheless, GBS is still the leading cause of severe disease among newborns, notably because the incidence of GBS late-onset disease (LOD) is not affected by IAP. Another strategy such as maternal immunization for prevention of both EOD/LOD is highly desirable worldwide. Aiming to describe GBS epidemiology and characterization of relevant epidemiological markers for vaccine development, surveillance of isolates causing neonatal disease is needed. We provide here results from the Belgian surveillance organized by the National Reference Centre(NRC). Methods: A total of 292 strains of GBS isolated from blood culture/cerebro-spinal fluid of newborns with invasive disease (149 EOD; 143 LOD) were sent to NRC by laboratories of a surveillance network, through years 2010-2017. Capsular-polysaccharide (CPS)-typing and pili-typing were performed with multiplex PCR assays. Multilocus sequence-typing and assignment to the hypervirulent clonal-complex (CC)17 was determined. Results: CPS type III isolates were responsible for 38.9% (n=58) of EOD cases, followed mainly by types Ia, V and II (22.1%, 18.1%, 8.1%). LOD cases were mainly caused by type III isolates (n=107, 74.8%), followed by types Ia (12.6%), V, Ib, IV and II (4.2%, 3.5%,2.8%, 2.1%). These distributions did not vary during the study period. A pili type was assigned to all isolates: at least one pili gene, PI2a, PI2b, or a combination of genes PI1-PI2a and PI1-PI2b. In 2016-2017, the hypervirulent-clone CC17 accounted for 33.3% of EOD (70.4% of type III) and 67% of LOD (89% of type III). Conclusions: The Belgian CPS distributions of isolates from EOD/LOD were similar to European data. One or 2 of 3 pilus-genes were detected in all isolates. CPS type III was predominant in both EOD/LOD and was mainly represented by CC-17 strains.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pediatrics
Laboratory medicine & medical technology
Immunology & infectious disease
Author, co-author :
Melin, Pierrette  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
SACHELI, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire CNR
Lambotte, Olivia;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
DESCY, Julie ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire mycobactérie
HUYNEN, Pascale ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Sérolo. infectieuse et détection immuno. d'agents infectieux
MEEX, Cécile ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire bactériologie
Language :
English
Title :
Group B streptococcus neonatal invasive infections in Belgium 2010-2017, and characterization of isolated strains.
Publication date :
September 2019
Event name :
INMIS 2019 - 5th International Neonatal & Maternal Immunization Symposium
Event place :
Vancouver, BC, Canada
Event date :
September 15, 17, 2019
Audience :
International
Main work title :
INMIS 2019 Abstract book
Author, co-author :
INMIS, International Committee
Peer reviewed :
Peer reviewed
Available on ORBi :
since 05 February 2020

Statistics


Number of views
99 (8 by ULiège)
Number of downloads
29 (4 by ULiège)

Bibliography


Similar publications



Contact ORBi