Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing.

Nepal, Chirag; Hadzhiev, Yavor; Balwierz, Piotr J.; Tarifeño-Saldivia, Estefania; Cardenas, Ryan; Wragg, Joseph W.; Suzuki, Ana Maria M.; Carninci, Piero; Peers, Bernard; Lenhard, Boris; Andersen, Jesper B.; Müller, Ferenc

doi:10.1038/s41467-019-13687-0

Article (Scientific journals)

Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing.

Nepal, Chirag; Hadzhiev, Yavor; Balwierz, Piotr J. et al.

2020 • In Nature Communications, 11 (1), p. 168

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/244461

DOI
10.1038/s41467-019-13687-0

PubMed
31924754

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Abstract :

[en] Variations in transcription start site (TSS) selection reflect diversity of preinitiation complexes and can impact on post-transcriptional RNA fates. Most metazoan polymerase II-transcribed genes carry canonical initiation with pyrimidine/purine (YR) dinucleotide, while translation machinery-associated genes carry polypyrimidine initiator (5’-TOP or TCT). By addressing the developmental regulation of TSS selection in zebrafish we uncovered a class of dual-initiation promoters in thousands of genes, including snoRNA host genes. 5’-TOP/TCT initiation is intertwined with canonical initiation and used divergently in hundreds of dual-initiation promoters during maternal to zygotic transition. Dual-initiation in snoRNA host genes selectively generates host and snoRNA with often different spatio-temporal expression. Dual-initiation promoters are pervasive in human and fruit fly, reflecting evolutionary conservation. We propose that dual-initiation on shared promoters represents a composite promoter architecture, which can function both coordinately and divergently to diversify RNAs.

Disciplines :

Genetics & genetic processes

Author, co-author :

Nepal, Chirag

Hadzhiev, Yavor

Balwierz, Piotr J.

Tarifeño-Saldivia, Estefania

Cardenas, Ryan

Wragg, Joseph W.

Suzuki, Ana Maria M.

Carninci, Piero

Peers, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Stem Cells-Zebrafish Dev. & Disease Model

Lenhard, Boris

Andersen, Jesper B.

Müller, Ferenc; Birmingham University

Language :

English

Title :

Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing.

Publication date :

10 January 2020

Journal title :

Nature Communications

eISSN :

2041-1723

Publisher :

Nature Publishing Group, United Kingdom

Volume :

Issue :

Pages :

168

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.nature.com/articles/s41467-019-13687-0

Available on ORBi :

since 04 February 2020

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