[en] Variations in transcription start site (TSS) selection reflect diversity of preinitiation complexes and can impact on post-transcriptional RNA fates. Most metazoan polymerase II-transcribed genes carry canonical initiation with pyrimidine/purine (YR) dinucleotide, while translation machinery-associated genes carry polypyrimidine initiator (5’-TOP or TCT). By addressing the developmental regulation of TSS selection in zebrafish we uncovered a class of dual-initiation promoters in thousands of genes, including snoRNA host genes. 5’-TOP/TCT initiation is intertwined with canonical initiation and used divergently in hundreds of dual-initiation promoters during maternal to zygotic transition. Dual-initiation in snoRNA host genes selectively generates host and snoRNA with often different spatio-temporal expression. Dual-initiation promoters are pervasive in human and fruit fly, reflecting evolutionary conservation. We propose that dual-initiation on shared promoters represents a composite promoter architecture, which can function both coordinately and divergently to diversify RNAs.
Disciplines :
Genetics & genetic processes
Author, co-author :
Nepal, Chirag
Hadzhiev, Yavor
Balwierz, Piotr J.
Tarifeño-Saldivia, Estefania
Cardenas, Ryan
Wragg, Joseph W.
Suzuki, Ana Maria M.
Carninci, Piero
Peers, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Stem Cells-Zebrafish Dev. & Disease Model
Lenhard, Boris
Andersen, Jesper B.
Müller, Ferenc; Birmingham University
Language :
English
Title :
Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing.
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