Absence of correlation between follicular fluid volume and follicular granulocyte colony-stimulating factor, a predictor of embryo implantation and successful delivery.
[en] Follicular granulocyte colony-stimulating factor (G-CSF) is a documented marker of embryo implantation potential. The primary objective was to determine whether follicular G-CSF levels correlate with follicular fluid volume. The secondary objectives were to assess whether follicular G-CSF is associated with oocyte maturity at the time of harvest and with delivery rate after fresh or frozen embryo transfer. Thirty-two patients undergoing intracytoplasmic sperm injection (ICSI) cycles were recruited (Centre de Procreation Medicalement Assistee (CPMA), University of Liege, Belgium). A total of 211 follicular fluid (FF) samples were individually collected at the time of oocyte harvest. FF volume was recorded, and G-CSF concentration was assessed by ELISA. The embryos were individually cultured in vitro. Their implantation and live birth rates were recorded after fresh and frozen embryo transfers. The follicular fluid volume did not correlate with the follicular G-CSF concentration. There were no differences in follicular G-CSF levels between mature and immature oocytes. The probability of successful implantation and delivery was increased for embryos with FF containing a high G-CSF concentration. There was a trend toward lower follicular G-CSF levels in cases of miscarriage. Therefore, follicular fluid volume cannot be a substitute for follicular G-CSF as a marker of embryo implantation ability.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
NOEL, Laure ; Centre Hospitalier Universitaire de Liège - CHU > Département de gynécologie-obstétrique > Service de gynécologie-obstétrique (CHR)
Donneau, Anne-Françoise ; Université de Liège - ULiège > Département des sciences de la santé publique > Biostatistique
JOUAN, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Département de gynécologie-obstétrique > Centre de procréation médicalement assistée (CPMA)
SCHOENEN, Sophie ; Centre Hospitalier Universitaire de Liège - CHU > Département de gynécologie-obstétrique > Service de gynécologie-obstétrique
Ledee, Nathalie
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Nisolle, Michelle ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Munaut, Carine ; Université de Liège - ULiège > Cancer-Tumours and development biology
Language :
English
Title :
Absence of correlation between follicular fluid volume and follicular granulocyte colony-stimulating factor, a predictor of embryo implantation and successful delivery.
Patrizio P, Sakkas D. From oocyte to baby: a clinical evaluation of the biological efficiency of in vitro fertilization. Fertil Steril. 2009;91:1061–1066.
European IVFmC, European Society of Human R, Embryology, et al. Assisted reproductive technology in Europe, 2013: results generated from European registers by ESHRE. Hum Reprod. 2017;32:1957–1973.
Paternot G, Wetzels AM, Thonon F, et al. Intra- and interobserver analysis in the morphological assessment of early stage embryos during an IVF procedure: a multicentre study. Reprod Biol Endocrinol. 2011;9:127.
Alpha Scientists in Reproductive M, Embryology ESIGo. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26:1270–1283.
Norwitz ER, Edusa V, Park JS. Maternal physiology and complications of multiple pregnancy. Semin Perinatol. 2005;29:338–348.
Ledee N, Frydman R, Osipova A, et al. Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles. Fertil Steril. 2011;95:94–98.
Ledee N, Gridelet V, Ravet S, et al. Impact of follicular G-CSF quantification on subsequent embryo transfer decisions: a proof of concept study [Research Support, Non-U.S. Gov't]. Hum Reprod. 2013;28:406–413.
Ledee N, Lombroso R, Lombardelli L, et al. Cytokines and chemokines in follicular fluids and potential of the corresponding embryo: the role of granulocyte colony-stimulating factor. Hum Reprod. 2008;23:2001–2009.
Ledee N, Munaut C, Serazin V, et al. Performance evaluation of microbead and ELISA assays for follicular G-CSF: a non-invasive biomarker of oocyte developmental competence for embryo implantation [Research Support, Non-U.S. Gov't]. J Reprod Immunol. 2010;86:126–132.
Ledee N, Petitbarat M, Rahmati M, et al. New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side [Review]. J Reprod Immunol. 2011;88:118–123.
Rienzi L, Vajta G, Ubaldi F. Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum Reprod Update. 2011;17:34–45.
Coticchio G, Sereni E, Serrao L, et al. What criteria for the definition of oocyte quality? Ann N Y Acad Sci. 2004;1034:132–144.
Yanez LZ, Han J, Behr BB, et al. Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization. Nat Commun. 2016;7:10809.
Kyei-Mensah A, Zaidi J, Pittrof R, et al. Transvaginal three-dimensional ultrasound: accuracy of follicular volume measurements. Fertil Steril. 1996;65:371–376.
Amer A, Hammadeh ME, Kolkailah M, et al. Three-dimensional versus two-dimensional ultrasound measurement of follicular volume: are they comparable? Arch Gynecol Obstet. 2003;268:155–157.
Somers F, Mc Hugh J. Performance verification of the Diafert® Granulocyte Colony-Stimulating Factor immunoassay. Paper presented at: COGI 2016. Proceedings of the 24th World Congress on Controversies in Obstetrics, Gynecology & Infertility (COGI); 2016 Nov 10-13; Amsterdam, the Netherlands.
Tournaye H, D’Hooghe T, Verheyen G, et al. Clinical performance of Diafert® to determine Granulocyte Colony Stimulating Factor concentration in follicular fluid as a predictor of implantation during In Vitro Fertilization. Paper presented at: COGI 2016. Proceedings of the 24th World Congress on Controversies in Obstetrics, Gynecology & Infertility (COGI); 2016 Nov 10-13; Amsterdam, the Netherlands.
Tournaye H, D'Hooghe T, Verheyen G, et al. Clinical performance of a specific granulocyte colony stimulating factor ELISA to determine its concentration in follicular fluid as a predictor of implantation success during in vitro fertilization. Gynecol Endocrinol. 2019;22:1–5.
Salmassi A, Schmutzler AG, Huang L, et al. Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells. Fertil Steril. 2004;81:786–791.
Yanagi K, Makinoda S, Fujii R, et al. Cyclic changes of granulocyte colony-stimulating factor (G-CSF) mRNA in the human follicle during the normal menstrual cycle and immunolocalization of G-CSF protein. Hum Reprod. 2002;17:3046–3052.
Gaafar TM, Hanna MO, Hammady MR, et al. Evaluation of cytokines in follicular fluid and their effect on fertilization and pregnancy outcome. Immunol Invest. 2014;43:572–584.
Niu Z, Ye Y, Xia L, et al. Follicular fluid cytokine composition and oocyte quality of polycystic ovary syndrome patients with metabolic syndrome undergoing in vitro fertilization. Cytokine. 2017;91:180–186.
Salmassi A, Schmutzler AG, Schaefer S, et al. Is granulocyte colony-stimulating factor level predictive for human IVF outcome? Hum Reprod. 2005;20:2434–2440.
Simonetti S, Veeck LL, Jones HW., Jr.Correlation of follicular fluid volume with oocyte morphology from follicles stimulated by human menopausal gonadotropin. Fertil Steril. 1985;44:177–180.
Wirleitner B, Okhowat J, Vistejnova L, et al. Relationship between follicular volume and oocyte competence, blastocyst development and live-birth rate: optimal follicle size for oocyte retrieval. Ultrasound Obstet Gynecol. 2018;51:118–125.
Kahyaoglu I, Yilmaz N, Timur H, et al. Granulocyte colony-stimulating factor: a relation between serum and follicular fluid levels and in-vitro fertilization outcome in patients with polycystic ovary syndrome. Cytokine. 2015;74:113–116.
