Evaluation of the Abbott RealTime quantitative CMV and EBV assays using the maxCycle protocol in a laboratory automation context

Bontems, Sébastien; BOREUX, Raphaël; CAPRARO, Valérie; Huynen, Pascale; DESCY, Julie; Melin, Pierrette; Hayette, Marie-Pierre; MEEX, Cécile

doi:10.1016/j.jviromet.2019.05.007

Article (Scientific journals)

Evaluation of the Abbott RealTime quantitative CMV and EBV assays using the maxCycle protocol in a laboratory automation context

Bontems, Sébastien; BOREUX, Raphaël; CAPRARO, Valérie et al.

2019 • In Journal of Virological Methods, 270, p. 137-145

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/243565

DOI
10.1016/j.jviromet.2019.05.007

PubMed
31121188

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Keywords :

Abbott maxCycle; Automation; CMV; EBV

Abstract :

[en] Real-time PCR are often used for the diagnosis and monitoring of Cytomegalovirus (CMV)and Epstein-Barr virus (EBV)infections in susceptible populations. In this context, we evaluated the analytical performances of the Abbott RealTime CMV/EBV maxCycle protocol automated on the m2000 platform (Abbott). It was compared to our routinely-used procedure consisting of a NucleoMag® DNA extraction automated on a STARlet platform followed by manually processed CMV and EBV quantitative real-time PCR (Diagenode). In this study, we showed that both EBV assays exhibited a similar sensitivity but with a better precision for the EBV Abbott RealTime assay. For the CMV performances, the Abbott assay was more sensitive and more precise than our routine method. The use of WHO International Standards also indicated a slight underestimation of the viral loads (−0.25 log10 IU/mL and −0.21 log10 IU/mL for CMV and EBV assays respectively)while these were rather overestimated with the Starlet/Diagenode method (0.48 log10 IU/mL and 0.19 log10 IU/mL for CMV and EBV assays respectively). These trends were confirmed using relevant whole-blood clinical samples and external quality controls. The workflows were also compared and we highlighted a significant technician hands-on time reduction (−63%)using the Abbott CMV/EBV maxCycle automated protocol. © 2019 Elsevier B.V.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

Bontems, Sébastien ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

BOREUX, Raphaël ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire biologie moléculaire

CAPRARO, Valérie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Secteur commun biologie moléculaire

Huynen, Pascale ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

DESCY, Julie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire mycobactérie

Melin, Pierrette ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie

Hayette, Marie-Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie

MEEX, Cécile ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire bactériologie

Language :

English

Title :

Evaluation of the Abbott RealTime quantitative CMV and EBV assays using the maxCycle protocol in a laboratory automation context

Publication date :

2019

Journal title :

Journal of Virological Methods

ISSN :

0166-0934

eISSN :

1879-0984

Publisher :

Elsevier B.V.

Volume :

270

Pages :

137-145

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 January 2020

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Abeynayake, J., Johnson, R., Libiran, P., Sahoo, M.K., Cao, H., Bowen, R., Allen Chan, K.C., Le, Q.T., Pinsky, B.A., Commutability of the epstein-barr virus WHO international standard across two quantitative PCR methods. J. Clin. Microbiol. 52 (2014), 3802–3804, 10.1128/JCM.01676-14.
Andrews, P.A., Emery, V.C., Newstead, C., Summary of the British transplantation society guidelines for the prevention and management of CMV disease after solid organ transplantation. Transplantation 92 (2011), 1181–1187, 10.1097/TP.0b013e318235c7fc.
Bland, J., Altman, G., Measuring agreement in method comparison studies — a review. Adv. Rank. Sel. Mult. Comp. Reliab. 2802 (2007), 215–244, 10.1007/0-8176-4422-9_13.
Bontems, S., Hayette, M.-P., Descy, J., Huynen, P., Melin, P., Capraro, V., Boreux, R., Meex, C., Raw Data From the Validation of the Abbott RealTime CMV/EBV maxCycle Protocol Using WHO International Standards. n.d., Mendeley Data, v1, 2019 https://www.google.com/search?client=firefox-b-d&channel=trow&q=https//data.mendeley.com/datasets/gsjh22h3w7/draft%3Fa%3De79e170ef494-4149-845c-bbbbdfd088c4.
Caliendo, A.M., Shahbazian, M.D., Schaper, C., Ingersoll, J., Abdul-Ali, D., Boonyaratanakornkit, J., Pang, X.L., Fox, J., Preiksaitis, J., Schönbrunner, E.R., A commutable cytomegalovirus calibrator is required to improve the agreement of viral load values between laboratories. Clin. Chem. 55 (2009), 1701–1710, 10.1373/clinchem.2009.124743.
Croxatto, A., Prod'hom, G., Faverjon, F., Rochais, Y., Greub, G., Laboratory automation in clinical bacteriology: what system to choose?. Clin. Microbiol. Infect. 22 (2016), 217–235, 10.1016/j.cmi.2015.09.030.
Dauwalder, O., Landrieve, L., Laurent, F., de Montclos, M., Vandenesch, F., Lina, G., Does bacteriology laboratory automation reduce time to results and increase quality management?. Clin. Microbiol. Infect. 22 (2016), 236–243, 10.1016/j.cmi.2015.10.037.
Dioverti, M.V., Razonable, R.R., Cytomegalovirus. Microbiol. Spectr. 4 (2016), 1–26, 10.1128/microbiolspec.DMIH2-0022-2015.Correspondence.
Engelmann, I., Alidjinou, E.K., Lazrek, M., Pouillaude, J., Ogiez, J., Rose, F., Duhamel, A., Dewilde, A., Hober, D., Comparison of Two Commercial Quantitative PCR Assays for EBV DNA Detection and their Correlation with the First WHO International Standard for EBV. 2019, 529–536, 10.1099/jmm.0.000702.
Engelmann, I., Kazali, E., Lazrek, M., Ogiez, J., Pouillaude, J., Chazard, E., Dewilde, A., Hober, D., Comparison of two commercial quantitative PCR assays and correlation with the fi rst WHO International Standard for human CMV. Diagn. Microbiol. Infect. Dis. 91 (2018), 27–33, 10.1016/j.diagmicrobio.2017.12.021.
Felder, R.A., Boyd, J.C., Savory, J., Margrey, K., Martinez, A., Vaughn, D., Robotics in the clinical laboratory. Clin. Lab. Med. 8 (1988), 699–711.
Fryer, J.F., Heath, A.B., Minor, P.D., Kessler, H., Rawlinson, W., Boivin, G., Preiksaitis, J., Pang, X.-L., Barranger, C., Alain, S., Bressollette-Bodin, C., Hamprecht, K., Grewing, T., Constantoulakis, P., Ghisetti, V., Capobianchi, M.R., Abbate, I., Olivo, C., Lazzarotto, T., Baldanti, F., Inoue, N., Müller, F., Corcoran, C., Hardie, D., Prieto, J., Schuurman, R., van Loon, A., Ho, S., Hillyard, D., Hodinka, R., Louise Landry, M., Caliendo, A., Lurain, N., Sung, L., Gulley, M., Atkinson, C., Bible, J., Guiver, M., A collaborative study to establish the 1st WHO International Standard for human cytomegalovirus for nucleic acid amplification technology. Biologicals 44 (2016), 242–251, 10.1016/j.biologicals.2016.04.005.
Fryer, J.F., Heath, A.B., Wilkinson, D.E., Minor, P.D., A collaborative study to establish the 1st WHO International Standard for Epstein–Barr virus for nucleic acid amplification techniques. Biologicals 44 (2016), 423–433, 10.1016/j.biologicals.2016.04.010.
Fryer, J.F., Morris, C.L., Almond, N.M., Minor, P.D., Commutability of the first world health organization international standard for human cytomegalovirus: standard or assay. J. Clin. Microbiol., 54, 2016, 3073, 10.1128/JCM.01706-16.
Furione, M., Rognoni, V., Cabano, E., Baldanti, F., Kinetics of human cytomegalovirus (HCMV)DNAemia in transplanted patients expressed in international units as determined with the Abbott RealTime CMV assay and an in-house assay. J. Clin. Virol. 55 (2012), 317–322, 10.1016/j.jcv.2012.08.017.
Gulley, M.L., Tang, W., Using Epstein-Barr viral load assays to diagnose, monitor, and prevent posttransplant lymphoproliferative disorder. Clin. Microbiol. Rev. 23 (2010), 350–366, 10.1128/CMR.00006-09.
Hawker, C.D., Laboratory automation: total and subtotal. Clin. Lab. Med. 27 (2007), 749–770, 10.1016/j.cll.2007.07.010.
Hayden, R.T., Preiksaitis, J., Tong, Y., Pang, X., Sun, Y., Tang, L., Cook, L., Pounds, S., Fryer, J., Caliendo, A.M., Commutability of the first world health organization international standard for human cytomegalovirus. J. Clin. Microbiol. 53 (2015), 3325–3333, 10.1128/JCM.01495-15.
Hayden, R.T., Shahbazian, M.D., Valsamakis, A., Boonyaratanakornkit, J., Cook, L., Pang, X.L., Preiksaitis, J.K., Schönbrunner, E.R., Caliendo, A.M., Multicenter evaluation of a commercial cytomegalovirus quantitative standard: effects of commutability on interlaboratory concordance. J. Clin. Microbiol. 51 (2013), 3811–3817, 10.1128/JCM.02036-13.
Heemann, U., Abramowicz, D., Spasovski, G., Vanholder, R., Endorsement of the kidney disease improving global outcomes (KDIGO)guidelines on kidney transplantation: a European renal best practice (ERBP)position statement. Nephrol. Dial. Transplant. 26 (2011), 2099–2106, 10.1093/ndt/gfr169.
Kim, K.Y., Le, Q.-T., Yom, S.S., Ng, R.H.W., Chan, K.C.A., Bratman, S.V., Welch, J.J., Divi, R.L., Petryshyn, R.A., Conley, B.A., Clinical utility of epstein-barr virus DNA testing in the treatment of nasopharyngeal carcinoma patients. Int. J. Radiat. Oncol. Biol. Phys. 98 (2017), 996–1001, 10.1016/j.ijrobp.2017.03.018.
Kotton, C.N., Kumar, D., Caliendo, A.M., Asberg, A., Chou, S., Danziger-Isakov, L., Humar, A., Updated international consensus guidelines on the management of cytomegalovirus in solid-organ transplantation. Transplantation 96 (2013), 333–360, 10.1097/TP.0b013e31829df29d.
Lucic, D., Jones, S., Wiesneth, R., Barry, C., Webb, E., Belova, L., Dolan, P., Ho, S., Abravaya, K., Cloherty, G., Impact of the new Abbott mPLUS feature on clinical laboratory efficiencies of Abbott RealTime assays for detection of HIV-1, hepatitis C virus, hepatitis B virus, Chlamydia trachomatis, and Neisseria gonorrhoeae. J. Clin. Microbiol. 51 (2013), 4050–4054, 10.1128/JCM.01672-13.
Martinez, O.M., Krams, S.M., The immune response to Epstein Barr virus and implications for posttransplant lymphoproliferative disorder. Transplantation 101 (2017), 2009–2016, 10.1097/TP.0000000000001767.
Navarro, D., San-Juan, R., Manuel, O., Giménez, E., Fernández-Ruiz, M., Hirsch, H.H., Grossi, P.A., Aguado, J.M., Cytomegalovirus infection management in solid organ transplant recipients across European centers in the time of molecular diagnostics: an ESGICH survey. Transpl. Infect. Dis., 2017, 1–12, 10.1111/tid.12773.
Preiksaitis, J.K., Hayden, R.T., Tong, Y., Pang, X.L., Fryer, J.F., Heath, A.B., Cook, L., Petrich, A.K., Yu, B., Caliendo, A.M., Are we there yet? Impact of the first international standard for cytomegalovirus DNA on the harmonization of results reported on plasma samples. Clin. Infect. Dis. 63 (2016), 583–589, 10.1093/cid/ciw370.
R Core Team, R: a Language and Environment for Statistical Computing. 2016, R Foundation for Statistical Computing, Vienna, Austria n.d., [WWW Document]. URL https://www.r-project.org/ https://www.r-project.org/.
Ruf, S., Wagner, H.-J., Determining EBV load: current best practice and future requirements. Expert Rev. Clin. Immunol. 9 (2013), 139–151, 10.1586/eci.12.111.
Salmona, M., Fourati, S., Feghoul, L., Scieux, C., Thiriez, A., Simon, F., Resche-Rigon, M., LeGoff, J., Automated quantification of Epstein–Barr Virus in whole blood of hematopoietic stem cell transplant patients using the Abbott m2000 system. Diagn. Microbiol. Infect. Dis. 85 (2016), 428–432, 10.1016/j.diagmicrobio.2016.04.017.
Schnepf, N., Scieux, C., Resche-Riggon, M., Feghoul, L., Xhaard, A., Gallien, S., Molina, J.M., Socié, G., Viglietti, D., Simon, F., Mazeron, M.C., LeGoff, J., Fully automated quantification of cytomegalovirus (CMV)in whole blood with the new sensitive Abbott realTime CMV assay in the era of the CMV international standard. J. Clin. Microbiol. 51 (2013), 2096–2102, 10.1128/JCM.00067-13.
Semenova, T., Lupo, J., Alain, S., Perrin-Confort, G., Grossi, L., Dimier, J., Epaulard, O., Morand, P., Germi, R., French EBV Study Group, Multicenter evaluation of whole-blood epstein-barr viral load standardization using the WHO international standard. J. Clin. Microbiol. 54 (2016), 1746–1750, 10.1128/JCM.03336-15.
Tang, L., Sun, Y., Buelow, D., Gu, Z., Caliendo, A.M., Pounds, S., Hayden, R.T., Quantitative assessment of commutability for clinical viral load testing using a digital PCR-based reference standard. J. Clin. Microbiol. 54 (2016), 1616–1623, 10.1128/JCM.03346-15.
Torre-Cisneros, J., Aguado, J.M., Caston, J.J., Almenar, L., Alonso, A., Cantisán, S., Carratalá, J., Cervera, C., Cordero, E., Fariñas, M.C., Fernández-Ruiz, M., Fortún, J., Frauca, E., Gavaldá, J., Hernández, D., Herrero, I., Len, O., Lopez-Medrano, F., Manito, N., Marcos, M.A., Martín-Dávila, P., Monforte, V., Montejo, M., Moreno, A., Muñoz, P., Navarro, D., Pérez-Romero, P., Rodriguez-Bernot, A., Rumbao, J., San Juan, R., Vaquero, J.M., Vidal, E., Management of cytomegalovirus infection in solid organ transplant recipients: SET/GESITRA-SEIMC/REIPI recommendations. Transplant. Rev. 30 (2016), 119–143, 10.1016/j.trre.2016.04.001.
Tsai, H.-P., Tsai, Y.-Y., Lin, I.-T., Kuo, P.-H., Chen, T.-Y., Chang, K.-C., Wang, J.-R., Comparison of two commercial automated nucleic acid extraction and integrated quantitation real-time PCR platforms for the detection of cytomegalovirus in plasma. PLoS One, 11, 2016, e0160493, 10.1371/journal.pone.0160493.
Vinuesa, V., Solano, C., Giménez, E., Navarro, D., Comparison of the artus Epstein-Barr virus (EBV)PCR kit and the Abbott RealTime EBV assay for measuring plasma EBV DNA loads in allogeneic stem cell transplant recipients. Diagn. Microbiol. Infect. Dis., 2017, 10.1016/j.diagmicrobio.2017.02.010.
Wattles, B., Kim, A., Cheerva, A., Lukas, K., Elder, J., Cytomegalovirus treatment in pediatric hematopoietic stem cell transplant patients. J. Pediatr. Hematol. Oncol. 39 (2017), 241–248, 10.1097/MPH.0000000000000730.
Wolff, D.J., Heaney, D.L., Neuwald, P.D., Stellrecht, K.A., Press, R.D., Multi-site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization. J. Mol. Diagn. 11 (2009), 87–92, 10.2353/jmoldx.2009.080097.