ATAT1-enriched vesicles promote microtubule acetylation via axonal transport.

[en] Microtubules are polymerized dimers of alpha- and beta-tubulin that underlie a broad range of cellular activities. Acetylation of alpha-tubulin by the acetyltransferase ATAT1 modulates microtubule dynamics and functions in neurons. However, it remains unclear how this enzyme acetylates microtubules over long distances in axons. Here, we show that loss of ATAT1 impairs axonal transport in neurons in vivo, and cell-free motility assays confirm a requirement of alpha-tubulin acetylation for proper bidirectional vesicular transport. Moreover, we demonstrate that the main cellular pool of ATAT1 is transported at the cytosolic side of neuronal vesicles that are moving along axons. Together, our data suggest that axonal transport of ATAT1-enriched vesicles is the predominant driver of alpha-tubulin acetylation in axons.

Disciplines :

Neurology

Author, co-author :

Even, Aviel

Morelli, Giovanni

Broix, Loïc ; Université de Liège - ULiège > Stem Cells-Molecular Regulation of Neurogenesis

Scaramuzzino, Chiara

Turchetto, Silvia ; Université de Liège - ULiège > Stem Cells-Molecular Regulation of Neurogenesis

Gladwyn-Ng, Ivan

Le Bail, Romain ; Université de Liège - ULiège > Stem Cells-Molecular Regulation of Neurogenesis

Shilian, Michal

Freeman, Stephen

Magiera, Maria M.

More authors (10 more)

Language :

English

Title :

ATAT1-enriched vesicles promote microtubule acetylation via axonal transport.

Publication date :

2019

Journal title :

Science Advances

eISSN :

2375-2548

Publisher :

American Association for the Advancement of Science (AAAS), Washington, United States

Volume :

Issue :

Pages :

eaax2705

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Copyright (c) 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

Available on ORBi :

since 16 January 2020

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