Letter to the editor (Scientific journals)
The centrosome protein AKNA regulates neurogenesis via microtubule organization
Camargo Ortega, G.; Falk, S.; Johansson, P. A. et al.
2019In Nature, 567 (7746), p. 113-117
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Abstract :
[en] The expansion of brain size is accompanied by a relative enlargement of the subventricular zone during development. Epithelial-like neural stem cells divide in the ventricular zone at the ventricles of the embryonic brain, self-renew and generate basal progenitors 1 that delaminate and settle in the subventricular zone in enlarged brain regions 2 . The length of time that cells stay in the subventricular zone is essential for controlling further amplification and fate determination. Here we show that the interphase centrosome protein AKNA has a key role in this process. AKNA localizes at the subdistal appendages of the mother centriole in specific subtypes of neural stem cells, and in almost all basal progenitors. This protein is necessary and sufficient to organize centrosomal microtubules, and promote their nucleation and growth. These features of AKNA are important for mediating the delamination process in the formation of the subventricular zone. Moreover, AKNA regulates the exit from the subventricular zone, which reveals the pivotal role of centrosomal microtubule organization in enabling cells to both enter and remain in the subventricular zone. The epithelial-to-mesenchymal transition is also regulated by AKNA in other epithelial cells, demonstrating its general importance for the control of cell delamination. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.
Disciplines :
Neurology
Author, co-author :
Camargo Ortega, G.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, Germany, Graduate School of Systemic Neurosciences, Biocenter, Ludwig-Maximilians University, Munich, Germany
Falk, S.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, Germany
Johansson, P. A.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, Germany, Laboratory of Molecular Neurogenetics, Lund University, Lund, Sweden
Peyre, E.;  GIGA-Stem Cells, Molecular regulation of neurogenesis, University of Liège, Liège, Belgium
Broix, Loïc ;  Université de Liège - ULiège > Neurosciences-Molecular Regulation of Neurogenesis
Sahu, S. K.;  Institute of Molecular Biology (IMB), Mainz, Germany
Hirst, W.;  IRI for the Life Sciences, Humboldt University, Berlin, Germany, Research School of Biology, The Australian National University, Canberra, ACT, Australia
Schlichthaerle, T.;  Max Planck Institute of Biochemistry, Martinsried, Germany, Department of Physics and Center for Nanoscience, Ludwig Maximilians University, Munich, Germany
De Juan Romero, C.;  Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d’Alacant, Spain
Draganova, K.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, Germany
Vinopal, S.;  Laboratory for Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany
Chinnappa, K.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d’Alacant, Spain
Gavranovic, A.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany
Karakaya, T.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany
Steininger, T.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany
Merl-Pham, J.;  Research Unit Protein Science, Helmholtz Centre Munich, German Research Center for Environmental Health, Munich, Germany
Feederle, R.;  Institute for Diabetes and Obesity, Monoclonal Antibody Core Facility, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, SYNERGY, Excellence Cluster of Systems Neurology, Biomedical Center, Ludwig-Maximilians University, Munich, Germany
Shao, W.;  Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States, BCMB Allied Graduate Program, Weill Cornell Medical College, New York, NY, United States
Shi, S.-H.;  Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States, BCMB Allied Graduate Program, Weill Cornell Medical College, New York, NY, United States
Hauck, S. M.;  Research Unit Protein Science, Helmholtz Centre Munich, German Research Center for Environmental Health, Munich, Germany
Jungmann, R.;  Max Planck Institute of Biochemistry, Martinsried, Germany, Department of Physics and Center for Nanoscience, Ludwig Maximilians University, Munich, Germany
Bradke, F.;  Laboratory for Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany
Borrell, V.;  Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d’Alacant, Spain
Geerlof, A.;  Protein Expression and Purification Facility, Institute of Structural Biology, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany
Reber, S.;  IRI for the Life Sciences, Humboldt University, Berlin, Germany, University of Applied Sciences, Berlin, Germany
Tiwari, V. K.;  Institute of Molecular Biology (IMB), Mainz, Germany
Huttner, W. B.;  Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Wilsch-Bräuninger, M.;  Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Nguyen, Laurent  ;  Université de Liège - ULiège > Neurosciences-Molecular Regulation of Neurogenesis
Götz, M.;  Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany, Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, Germany, Max Planck Institute of Biochemistry, Martinsried, Germany, SYNERGY, Excellence Cluster of Systems Neurology, Biomedical Center, Ludwig-Maximilians University, Munich, Germany
More authors (20 more) Less
Language :
English
Title :
The centrosome protein AKNA regulates neurogenesis via microtubule organization
Publication date :
2019
Journal title :
Nature
ISSN :
0028-0836
eISSN :
1476-4687
Publisher :
Nature Publishing Group
Volume :
567
Issue :
7746
Pages :
113-117
Peer reviewed :
Peer Reviewed verified by ORBi
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